主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (2): 133-140.doi: 10.3969/j.issn.1006-2157.2020.02.007

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Intervention of Compound Uncaria Tablets on myocardial differential protein in spontaneously hypertensive rats based on iTRAQ technology*

Wang Jianzhang, Yu Yunfeng, Zhou Manli, Feng Yu, Guo Zhihua, Jian Weixiong#   

  1. Hunan University of Chinese Medicine, Hunan 410208, China
  • Received:2019-09-02 Published:2020-03-24
  • Contact: A/Prof.Jian Weixiong,Ph. D., Hunan University of Chinese Medicine, Hunan 410208, China.Email: daxiong20001977@163.com
  • Supported by:
    National Natural Science Foundation of China(No.81774207);National Major Scientific and Technological SpecialProject for Significant New Drug Developmnet(No.2019ZX09301103);National Natural Science Foundation of Hunan Province(No. 2018JJ2291)

Abstract: Objective To study the protein changes in the intervention of the Compound Uncaria Tablets in Spontaneously Hypertensive Rats (SHR) and the potential mechanism of left ventricular hypertrophy in hypertension. Methods 16SHRs were divided into two groups. The treatment group was gavaged with the Compound Uncaria Tablets, and the control group was gavaged with distilled water. After 12 weeks of intervention, the myocardial tissue protein was taken and tested by iTRAQ assay analysis. By analyzing the enrichment of pathways, the differential proteins and pathways were obtained, and furtherly mapped using the STRING database. Results The pathway enrichment analysis of the differential protein indicated that there were 11 pathways. The iTRAQ analysis of the differential protein indicated that there were 6 up-regulated proteins: transgelatin, carbonic anhydrase 3, S-phase kinase-associated protein 1, C-reactive protein, fibrin 5, and C-1-tetrahydrofolate synthetase; and there were 12 down-regulated proteins: glutathione S-transferase, Ighg3, histidine trinucleotide binding protein 2, 60S ribosomal protein L4, 40S ribosomal protein S6, 60S ribosomal protein L13, 14-3-3 protein, protein LOC100911337, glutathione peroxidase 1, serine hydroxymethyltransferase, protein kinase B, and ubiquitin -40S ribosomal protein S27A.In the relational network drawn by string: 60S ribosomal protein L4, 40S ribosomal protein S6, 60S ribosomal protein L13, and ubiquitin-40S ribosomal protein S27A are most closely related. Conclusion The intervention of Compound Uncaria Tablets on hypertensive left ventricular hypertrophy is possibly mainly related to improving blood supply, optimizing energy metabolism, inhibiting abnormal growth of cardiomyocytes and increasing fibrin of blood vessels and heart. The occurrence of hypertensive left ventricular hypertrophy may be related to ribosomal proteins.

Key words: Compound Uncaria Tablets, hypertension, left ventricular hypertrophy, ribosomal protein, iTRAQ

CLC Number: 

  • R285.5