主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (2): 148-162.doi: 10.3969/j.issn.1006-2157.2020.02.009

• Chinese Medicinal Chemistry • Previous Articles     Next Articles

Quality evaluation of Perillae folium based on representation model of effective characteristic spectrum of reference*

Lu Dongbo1, Kong Jing1, Li Sitong1, Fan Yuanyuan1, Huang Yujuan1, Sun Rendi2, Huang Guangwei2, Liu Lulu1, Chen Siyu1, Gao Yuanyuan1, Jiang Yanyan1,3, Shi Renbing1,3#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;
    2 ShangHai Green Valley Shengmingyuan Pharmaceutical Co. , Ltd, Shanghai;
    3 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education, China.)
  • Received:2019-09-28 Published:2020-03-24
  • Contact: Prof. Shi Renbing, Ph. D., School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488. E-mail: shirb@ 126.com.
  • Supported by:
    National Sci-Tech Support Plan the 12th Five-Year Plan (No.2012BAI29B06); Beijing Municipal Education Commission Co-construction Scientific Research Base Project, Beijing University of Traditional Chinese Medicine Key Subject Open Project (No.2013-2DXKKF-23).

Abstract: Objective To establish the quality representation model, analysis method and quality evaluation based on the effective characteristic spectrum of reference of perillae folium, so as to evaluate the quality of perillae folium effectively and accurately. Methods To determine the perillae folium which has the effect of regulating blood lipid as the standard reference pieces. Taking effective characteristic index component as quality reference point. Using HPLC-PDA method to establish effective characteristic spectrum of reference, and obtained quality characterization information was used as the standard to evaluate the quality of perillae folium, and to prove the accuracy. Results Scutellarin, as the reference point, (retention time: 30.342 min; content: 0.573 4%; concentration: 0.057 34 g/L ), represented the quality information of characteristic spectrum of benchmark (S1) which was effective:14 characteristic peak retention time, peak area, relative retention time, relative peak area, six characteristic index components content, relative content and content conversion coefficient (caffeicacid,luteolin-7-O-glucuronide, apigenin-7-O-glucuronide, scutellarin, rosmarinic acid, oleanolic acid), which covers phenolic acids, flavonoids and triterpenoids. And reference substance of scutellarin, as the simulative datum point (rretention time: 29.759 min; concentration: 0.057 3 g/L), represented the quality information. The two results were consistent, and verified the feasibility of the test method and the accuracy of the test results.14 characteristic peaks appeared on the chromatograms of 14 batches of perillae folium and the conversion content of six characteristic index components was consistent with the measured content. Using the effective quality representation of reference information to evaluate the quality of 14 batches of perillae folium, and the quality of S8,S7,S12,S9,S13 were judged to be better. Conclusion The established quality representation model and analysis method based on the effective characteristic spectrum of reference of perillae folium can be used represent the quality of perillae folium simply, accurately and integrally. The established quality analysis model can be used to analyze the quality of perillae folium.

Key words: perillae folium, effective characteristic spectrum of reference, quality representation, quality standard, quality evaluation

CLC Number: 

  • R284.1