主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (3): 212-217.doi: 10.3969/j.issn.1006-2157.2020.03.006

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Songyu Anshen Fang on cAMP/PKA signaling pathways in hippocampus of insomnia rats*

Li Jingyi1, Huang Junshan2,3,4#, Chen Qin1, Wang Xiufeng2,3,4, Zhang Yu2,3,4, Zhang Yifan2,3,4   

  1. 1 Fujian University of Traditional Chinese Medicine, Fujian 350122, China;
    2 Fujian Academy of Traditional Chinese Medicine, Fujian 350003, China;
    3 Office of Inheritance of Fujian Famous Traditional Chinese Medicine Expert Huang Junshan, Fujian 350003, China;
    4 Fujian Key Laboratory of Sleep Medicine of Traditional Chinese Medicine, Fujian 350003, China
  • Received:2019-06-05 Published:2020-04-13
  • Contact: Prof. Huang Junshan, Ph.D., Chief Physician, Doctoral Supervisor. Fujian Academy of Traditional Chinese Medicine, Office of Inheritance of Fujian Famous Traditional Chinese Medicine Expert Huang Junshan, Fujian Key Laboratory of Sleep Medicine of Traditional Chinese Medicine. No.282 Wusi Road, Gulou District, Fuzhou 350003. E-mail:hjsh0825@163.com

Abstract: Objective To explore the effects of Songyu Anshen Fang (Radix et Rhizoma Nardostachyos and Radix Curcumae Spirit-calming Formula, SYASF) on 5-hydroxytryptamine 1A receptor (5-HT1AR) and cAMP signaling pathways in hippocampus of insomnia rats and to identify possible targets of such effects. Methods Fifty SD rats were randomly divided into control group, model group, low-dose SYASF group, high-dose SYASF group and buspirone group, with 10 rats in each group. Except the control group, rat models of liver constraint and insomnia were established in the other four groups with long-term tail-clip stimulation and intraperitoneal injection of chlorphenylalanine (PCPA). After the establishment of models, corresponding medicinals were administered to the low-dose SYASF group (8.5 g/kg), high-dose SYASF group (17 g/kg) and buspirone group (2.0 mg/kg) by gavage once a day for 14 consecutive days. Normal saline was given to the model group and the control group with the same frequency and duration. The general state of rats was observed during administration. By the end of administration, changes in body mass and results of open-field tests of the rats were observed and expressions of genes and proteins of 5-HT1AR, cAMP and PKA in all groups were detected. Results Compared with the control group, the time spent in the central area, number of grooming and number of fecal particles of the model group in the open field test were increased (P<0.05), and the expressions of 5-HT1AR, cAMP, PKA mRNA and protein in hippocampus of the model group were all decreased (P<0.05). Compared with the model group, the time spent in the central area, number of grooming and number of fecal particles of the high-dose SYASF group were reduced, while the mRNA and protein expressions of 5-HT1AR, cAMP, PKA increased (P<0.05). In the low-dose SYASF group, the time spent in the central area, number of grooming and number of fecal particles were decreased, while the mRNA and protein expressions of 5-HT1AR, PKA and cAMP were elevated (P<0.05), compared with the model group. There was no statistically significant difference between the high-dose SYASF group and the buspirone group (P>0.05). Conclusion SYASF can improve sleep of rats with liver constraint and insomnia, and its mechanism may be regulation through the cAMP/PKA signaling pathways mediated by 5-HT1AR in the hippocampus.

Key words: Songyu Anshen Fang, insomnia, 5-HT1A receptor, cAMP/PKA signaling pathway, rats

CLC Number: 

  • R285.5