主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (4): 304-309.doi: 10.3969/j.issn.1006-2157.2020.04.007

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects and mechanism of Qufeng Xuanfei formula on neurogenic inflammation in guinea pigs with cough hypersensitivity*

Wang Ying1, Shi Liqing2, Ma Jianling2#, Ji Kun2, Li Niuniu2, Wang Liyun2, Dong Shangjuan2, Wen Shaohui2, Luo Jingyue1   

  1. 1 Beijing University of Chinese Medicine,Beijing 100029, China;
    2 Dongfang Hospital, Beijing University of Chinese Medicine,Beijing 100078, China
  • Received:2019-10-31 Online:2020-04-30 Published:2020-05-14
  • Contact: Ma Jianling, A/Chief physician, Master Supervisor. Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078. E-mail: ponymjl@sina.com
  • Supported by:
    National Natural Science Foundation of China (No. 81373588); Beijing Municipal Natural Science Foundation (No. 7184224,No. 7192115)

Abstract: Objective To investigate the effects of Qufeng Xuanfei (Wind-expelling Lung-dispersing, QFXF) formula on neurogenic inflammation in guinea pigs with cough hypersensitivity. Methods 48 guinea pigs were randomly divided into the normal group, model group, capsazepine (intraperitoneal injection, 1 μmol/kg ) group and QFXF formula (5 g/kg) group (n=12 in each group). After treated with cyclophosphamide, ovalbumin and aluminium hydroxide to establish the model of cough hypersensitivity, the guinea pigs were orally given saline in the normal group and model group for 7 days. Then the number of cough was counted immediately after using atomized capsaicine stimulation; the airway resistance was measured with spirometer under different concentrations of acetylcholine chloride. Lung pathology was evaluated by using HE staining. The mRNA expression of lung TRPV1, SP, and CGRP was measured by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results The number of cough, the airway resistance and the mRNA expression of lung TRPV1, SP, and CGRP increased significantly in the model group compared with the normal group (P<0.05), and decreased significantly in the treatment groups compared with the model group (P<0.05). The mRNA expression of TRPV1, SP, and CGRP decreased significantly in the QFXF formula group compared with capsazepine group (P<0.05); The lung tissue in the model group showed apparent structure destruction and tissue inflammation compared with the normal group and the level of the destruction and inflammation was lower in the treatment groups compared with the model group. Conclusion Qufeng Xuanfei formula could reduce the level of the airway sensitivity in guinea pig models with cough hypersensitivity, and the mechanism may be related to relieving the airway neurogenic inflammation by inhibiting TRPV1 channel.

Key words: Qufeng Xuanfei formula, chronic cough, cough hypersensitivity, neurogenic inflammation, transient receptor potential V1, guinea pigs

CLC Number: 

  • R285.5