主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (4): 317-326.doi: 10.3969/j.issn.1006-2157.2020.04.009

• Chinese Medicinal Chemistry • Previous Articles     Next Articles

Quality standard of Polygonum Multiflorum processed with black bean juice*

Yu Yi1, Xie Jing2,3, Liu Ying2, Xu Yudi2, Zhang Jing2, Li Li3, Wang Guangzhong3, Xiao Yongqing2#   

  1. 1 Infinitus (China Co. Ltd, Guangzhou 510663, China;
    2 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    3 School of Pharmacy, Hubei University of Chinese Medicine, Hubei 430065, China]
  • Received:2019-12-17 Online:2020-04-30 Published:2020-05-14
  • Contact: Xiao Yongqing, Researcher, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16 Nanxiaojie Street, Dongzhimennei Road, Dongcheng District, Beijing 100700. E-mail: x.heqi@163.com
  • Supported by:
    National Natural Science Foundation of China (No.81773903); Special Founds for Basic Research by Public Welfare Research Institutions under the Central Government (No.ZZ13-YQ-042); Special Project for Basic Work of Science and Technology (No. 2014FY111100)

Abstract: Objective To establish the quality standard for Polygonum Multiflorum stewed with black bean juice. Methods TLC method was used for qualitative identification. The characteristic chromatograms and the contents of stilbene glucoside and anthraquinones were determined with HPLC. The content of polysaccharide was determined by using UV spectrophotometry, and the contents of D-fructose,D-glucose anhydrous and sucrose were determined by using HPLC-ELSD. Results The thin-layer spot of Polygonum Multiflorum stewed with black bean juice were clear and showed 4 color bands. The characteristic map detected 9 relatively stable chromatographic peaks as the indicator peak for qualitative identification. The content of stilbene glycoside should not be less than 0.70%. The total amount of emodin and emodin methyl ether should be no lower than 0.15%. The total contents of emodin-8-O-β-D-glucoside and emodin methyl ether-8-O-β-D-glucoside should not be below 0.01%. The content of polysaccharide was no less than 20%. The total amount of monosaccharide and disaccharides which were measured by D-fructose, D-glucose anhydrous and sucrose should not be less than 15%. Conclusion The quality standard of Polygonum Multiflorum stewed with Black Bean Juice is scientific and reasonable, which can be used for quality control of it.

Key words: Polygonum Multiflorum stewed with black bean juice, specific chromatogram, content determination, thin-layer chromatography (TLC), stilbene glycoside, anthraquinones, saccharide

CLC Number: 

  • R284.2