主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (4): 327-333.doi: 10.3969/j.issn.1006-2157.2020.04.010

• Acupuncture & Moxibustion • Previous Articles     Next Articles

Effect of electroacupuncture on GFAP in hippocampal dentate gyrus(DG) of rats with hyperlipidemia and middle cerebral ischemia*

Xue Naying, Ren Xiujun#   

  1. School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, China
  • Received:2019-11-11 Online:2020-04-30 Published:2020-05-14
  • Contact: Prof.Ren Xiujun, Chief physician, Master Supervisor. School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488. E-mail: rxiujun@163.com.
  • Supported by:
    National Natural Science Foundation in China (No. 81470200)

Abstract: Objective To observe the effect of electroacupuncture on GFAP in hippocampal dentate gyrus(DG) of rats with hyperlipidemia and middle cerebral artery thrombus. Methods Adult male SD rats were randomly divided into normal group, model group, sham-operation group, electroacupuncture 1 (EA1) group and electroacupuncture 2 (EA2) group. Hyperlipidemia stage: Rats in model and EA group were fed with high-fat diet for 42 days, while rats in EA1 group were needled at ST40 once a day for 7 days. Cerebral ischemia stage: Rats in model group and EA groups were induced by 50% FeCl3 to establish hyperlipidemia with cerebral ischemia. After operation, the EA groups needled at ST40 and GV20 once a day for 14 days. All rats were scored for neurological function. The levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL) and low-density lipoprotein (LDL) in serum were detected on the 1st, 7th and 14th days. The morphological changes of neuron cells were observed by using Hematoxylin-eosin (HE) staining. Immunohistochemical method was used to detect the expression of glial fibrillary acidic protein (GFAP) in DG and ischemic cerebral region. Results Compared with normal group, TC, TG and LDL in the model group increased (P<0.05), TG and LDL in the EA group decreased (P<0.01). In the model group, the neurological function score (NDS) was increased. HE staining showed obvious nuclear consolidation and contraction, scattered tissue arrangement. The expression of GFAP increased in the DG and ischemic cerebral regions (P<0.01). In EA group, NDS decreased. HE staining showed the appearance of neurons was improved. GFAP expressions in the DG and ischemic cerabral regions decreased at day 1, increased at day 7 and decreased again at day 14 after operation. Conclusion EA intervention in hyperlipidemia stage can reduce blood lipid level, improve neurological function, regulate activation of astrocytes and promote neurorepair after hyperlipidemia-ischemia injury.

Key words: electroacupuncture, hyperlipidemia, cerebral ischemia, astrocytes, hippocampus, rats

CLC Number: 

  • R245