主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (4): 334-342.doi: 10.3969/j.issn.1006-2157.2020.04.011

• Clinical Studies • Previous Articles     Next Articles

Correlation between tongue coating flora and serum inflammatory metabolism in gastric cancer patients of qi and yin deficiency syndrome*

Qin Yuanyuan1, Xu Shuo1, Xiang Chunjie1, Wu Juan1, Wang Ruiping2, Wu Zhenfeng3, Zhan Zhen1, Zhang Junfeng1#   

  1. 1 School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Jiangsu 210023, China;
    2 Department of Oncology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Jiangsu 210029, China;
    3 Department of Oncology Surgery, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Jiangsu 210029,China
  • Received:2019-08-03 Online:2020-04-30 Published:2020-05-14
  • Contact: Prof. Zhang Junfeng, Master Supervisor. School of Medicine & Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Jiangsu 210023. E-mail:zhangjunfeng5_5@163.com
  • Supported by:
    National Natural Science Foundation of China (No.81473458, No.81473593);National Clinical Research Base Construction of Chinese Medicine of State Administration of Traditional Chinese Medicine (No.JDZX2015089)

Abstract: Objective To explore the association between the inflammatory metabolic mechanism and tongue coating flora among the gastric cancer patients of qi and yin deficiency syndrome. Methods A hospital-based case-control study was conducted. 13 gastric cancer (GC) patients with Qi and Yin deficiency syndrome (QYDS) were selected as cases and 23 healthy subjects matched as controls. The tongue coating flora was measured by using high-throughput sequencing. The levels of 20 serum inflammatory factors were detected by using electro chemiluminescence immunoassay (ECLIA). Gas chromatography-mass spectrometer (GC-MS) was used to detect the serum metabolomics. Results Compared with the control group, the Shannon index of bacterial diversity of tongue coating in GC patients with QYDS was lower (P<0.05), but there was no significant difference in the alpha diversity of the tongue coating fungi (P>0.05). Based on LDA analysis, 3 bacterial genera (Caulobacter, Megamonas, Woodsholea) and 1 fungal genus (Schizothecium) seemed to be the marker flora of QYDS of GC. Levels of 15 metabolic molecules including serum glutamate, L-histidine and creatine were significantly higher in GC patients with QYDS. Compared with the control group, 8 serum inflammatory factors including IL-17α, TNF-α and IL-6 were significantly higher in GC patients with QYDS. Association analysis indicated IL-17α, IL-6, and TNF-α were inversely correlated with Fusobacterium and Porphyromonas (r<-0.4, P<0.05), Fusobacterium and Peptostreptococcus had significant negative correlation with serum glutamate metabolism (r<-0.4, P<0.05). Conclusions The structural difference of the tongue coating flora in GC patients with QYDS was related to serum inflammatory factors and metabolism, which could be used to explore the formation mechanism of qi and yin deficiency syndrome.

Key words: gastric cancer, qi and yin deficiency syndrome, tongue coating flora, metabolomics, inflammatory factors

CLC Number: 

  • R273