主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (5): 386-392.doi: 10.3969/j.issn.1006-2157.2020.05.007

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Protective effect and mechanism of Wuzi Yanzong Pill in rat sertoli cells under heat stress*

Liu Sijia, Chen Yucai, Hu Suqin, Li Chunrui, Guo Jian#   

  1. School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
  • Received:2019-11-11 Published:2020-06-08
  • Contact: Prof. Guo Jian, Ph. D., Doctoral Supervisor, School of Chinese Medicine, Beijing University of Chinese Medicine, Intersection of Yangguang Nandajie Road and Baiyang Donglu Road, Fangshan District, Beijing 102488, China. E-mail: guojian323@sina.com
  • Supported by:
    National Natural Science Foundation of China (No.81973584)Ethical review: Committee of Ethical Review on Medical and Experimental Animals in Beijing University of Chinese Medicine (No. BUCM-4-2019030701-1053)

Abstract: Objective To explore the pharmacological mechanism of Wuzi Yanzong Pill in the treatment of male spermatogenic dysfunction by network pharmacology in rat sertoli cells. Methods Targets of anti-spermatogenic dysfunction in Wuzi Yanzong Pill were screened and the biological function and signal pathway were analyzed by online database. The heat stress model was constructed with rat sertoli cells to detect changes in the production of reactive oxygen species (ROS) and the expression of Hsp70. Results The results of network pharmacology showed that Wuzi Yanzong Pill mainly improved male spermatogenesis dysfunction by regulating ROS level and extracellular stimulus response etc. The ROS fluorescence probe results showed that, compared with the blank group, the ROS production was significantly increased in the model group, and significantly decreased in the Wuzi Yanzong Pill group. Western blot results showed that the expression of Hsp70 in Wuzi Yanzong Pill group was higher than that in the blank group and lower than that in the model group. Conclusion Wuzi Yanzong Pill seems to protect rat sertoli cells under heat stress by reducing ROS production and Hsp70 expression.

Key words: Wuzi Yanzong Pill, heat stress, sertoli cells, network pharmacology, reactive oxygen species (ROS)

CLC Number: 

  • R285.5