主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (5): 402-407.doi: 10.3969/j.issn.1006-2157.2020.05.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Treatment of nonalcoholic steatohepatitis by regulating the methylation level of TLR4 gene with Xiaoyao San*

Xu Huichao, Gao Yan, Chen Hao, Hao Jianheng, Liu Jinfang, Liu Yang, Miao Yuchuan#   

  1. School of Basic Medicine, Shanxi University of Chinese Medicine, Shanxi 030619, China
  • Received:2019-11-17 Published:2020-06-08
  • Contact: Prof. Miao Yuchuan, Ph.D., Master’s Supervisor. School of Basic Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, China. E-mail: mych65@163.com
  • Supported by:
    National Natural Science Foundation of China (No.81470190); Scientific Research Project of Health Commission of Shanxi Province (No.2019089)Ethical review: Committee of Medical and Animal Ethical Review on Experimental Animals in Shanxi University of Chinese Medicine (No.2019LL016)

Abstract: Objective To explore the new mechanism of changes in TLR4 gene methylation levels in the treatment of liver stagnation and spleen deficiency pattern in non-alcoholic steatohepatitis (NASH) in rats with Xiaoyao San (Xiaoyao Powder). Methods 30 male SD rats were randomly divided into blank group, model group and Xiaoyao San group (n=10). Rats in the blank group were given normal diet for 28 weeks. followed by injection of normal saline for 4 weeks. In the model group and Xiaoyao San group, liver stagnation and spleen deficiency pattern model was established by feeding the rats with a high-fat and high-sugar diet with stimulations of chronic stress and irregular diets for 28 weeks In the last 4 weeks, the model group and Xiaoyao San group were injected with normal saline and Xiaoyao San solution respectively. At the end of 28 weeks, urine was collected from the three groups, and the liver and hippocampus tissues were taken to make pathological sections. The corresponding indicators were detected by real-time fluorescence quantitative PCR (qPCR), enzyme-linked immunosorbent assay (ELISA), and hydrogen sulfite sequencing PCR (BSP), etc. Results Compared with the model group, the symptoms of liver stagnation and spleen deficiency pattern were significantly alleviated with a decreased pattern score in the Xiaoyao San group. The numbers of fat vacuoles and infiltration degree of inflammatory cells in liver cells were significantly reduced. The TLR4 mRNA content in liver tissues was decreased, while the methylation level of TLR4 gene was increased (P<0.05). Conclusion Xiaoyao San may up-regulate the TLR4 gene methylation level and down-regulate the TLR4 mRNA expression in rats with liver stagnation and spleen deficiency pattern in non-alcoholic steatohepatitis, thus improving the degree of liver steatosis and alleviating inflammation and other symptoms. This seems to be the new mechanism for the treatment of such condition with Xiaoyao San.

Key words: Xiaoyao San (Xiaoyao Powder), nonalcoholic steatohepatitis (NASH), liver stagnation and spleen deficiency pattern, DNA methylation, TLR4

CLC Number: 

  • R285.5