主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (5): 408-413.doi: 10.3969/j.issn.1006-2157.2020.05.010

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effect of medicated serum of NaotaifangⅡ on microglia polarization induced by LPS*

Zhang Xiuli1,2, Lei Chang1,2, Liu Yang3, Ge Jinwen3#, Meng Pan1,2, Zhang Junyu1, Ren Yongzhen1, Zi Dong1, Zhu Wei1   

  1. 1. Technology Innovation Center, Hunan University of Chinese Medicine, Hunan 410208, China;
    2. State Key Laboratory Breeding Base of TCM Powder and Innovative Drugs Co-founded by Hunan Province and the Ministry of Science and Technology, Hunan 410208, China;
    3. Key Lab of Hunan Province for Prevention and Treatment of Cardio-cerebral Diseases with Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Hunan 410208, China
  • Received:2019-11-26 Published:2020-06-08
  • Contact: Prof. Ge Jinwen, Doctoral Supervisor. Key Lab of Hunan Province for Prevention and Treatment of Cardio-cerebral Diseases with Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, No. 300, Xueshi Road, Hanpu Science and Education Park, Yuelu District, Changsha 410208, China. E-mail: 40831556@qq.com
  • Supported by:
    National Natural Science Foundation for Young Scientists of China(No.81603608,8174174); Chinese Postdoctoral Science Foundation (No.2018M630905); Natural Science Project of Hunan Province (No.2099JJ50431)Ethical review: Committee of Ethical Review on Experimental Animals of First Affiliated Hospital of Hunan University of Chinese Medicine (No.ZYFY20180227)

Abstract: Objective To investigate the influence of medicated serum of Naotaifang Ⅱ on the morphology, M1/M2 polarization markers and inflammatory factors of HAPI cells induced by LPS. Methods The inflammation model of HAPI cell was induced by LPS. The cultured cells were divided into: blank group, model group, Naotaifang Ⅱ group, and minocycline group. Morphology and quantity of microglia were observed by High Content Imaging System. mRNA expression of M1-type markers (MCH II, iNOS, MCP-1, CD11b) and M2-type markers (Arg1, Mrc1, Ym1), pro-inflammatory factors (IL-1β, IL-6, IL-12, TNF-α) and anti-inflammatory factors (IL-4, IL-10, TNF-β) was detected by qRT-PCR. Results Compared with the blank group, in the model group, microglia cell body became larger while showing multipolarization or amoeba type with coarser and shorter synapse; the mRNA level of M1 markers and pro-inflammatory increased significantly (P< 0.01); moreover, the expression of M2 marker Mrc1 and anti-inflammatory factors IL-4 and TNF-β decreased dramatically (P<0.01). Compared with the model group, in NaotaifangⅡgroup, the cell swelling recovered, the synapses became longer, and the microglia increased. Besides, in NaotaifangⅡgroup, the mRNA expression of M1 markers MHCⅡ, iNOS and CD11b and the pro-inflammatory factors were significantly decreased (P<0.01), and the mRNA expression of M2 markers and anti-inflammatory factors were significantly increased (P<0.01). Compared with the minocycline group, the Naotaifang Ⅱ group showed no significant advantage in reducing the expression of M1 markers (P>0.05), while better effect on lowering the expression of pro-inflammatory factors IL-6, IL-12 and TNF-α (P<0.01) except IL-β. Besides, NaotaifangⅡcould better increase mRNA expression of M2 markers Mrc1, Ym1 and anti-inflammatory factors IL-4, TGF-β compared with the minocycline group. Conclusion Naotaifang seems to inhibit M1 polarization, promote M2 polarization by regulating cell morphology, M1/M2 markers and inflammatory factors. It could possibly enhance M2 markers and anti-inflammatory factors better than minocycline.

Key words: Naotaifang Ⅱ, microglia polarization, M1 markers, M2 markers, pro-inflammatory factors, anti-inflammatory factors

CLC Number: 

  • R285.5