主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (7): 583-591.doi: 10.3969/j.issn.1006-2157.2020.07.010

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Difference of intestinal ion transport function in rats with pattern of dampness retention due to spleen deficiency and analysis of coix seed's effects*

Han Xiaochun1, Lyu Jiajian1, Gu Liangzhen1, Tian Shen1, Yang Zhe1, Zhang Lu1, Guo Liangqing2, Zhao Haijun1, Wang Shijun1,3   

  1. 1 Shandong University of Traditional Chinese Medicine, Shandong 250355, China;
    2 Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Shandong 250014, China;
    3 Shandong Co-Innovation Center of Classic TCM formula, Shandong University of Traditional Chinese Medicine, Shandong 250355, China
  • Received:2019-07-29 Published:2020-08-11
  • Contact: Prof. Wang Shijun, Doctoral Supervisor. 4655 Daxue Road, University Technology Park,Changqing District, Jinan. Shandong University of Traditional Chinese Medicine, Shandong 256355, China. E-mail: pathology@163.com
  • Supported by:
    Shandong key research and development plan (No.2019GSF108210); National Natural Science Foundation of China (No.81703838,No.81874411); Shandong Province University Young Innovative Talent Introduction Plan (ljrz (2019) No.9-201,202); Natural Science Foundation of Shandong Province (No.ZR2019ZD23, No.ZR2019MH064);Team Project of "Young Innovative Science and Technology Plan" of Shandong University (No.2019kjk013)

Abstract: Objective To explore the changes of ion transport function in rats with pattern of dampness retention due to spleen deficiency (DRSD) and the mechanism of coix seed (Yiyiren, Semen Coicis) on strengthening the spleen and draining dampness. Methods The animal model of DRSD was established by swimming-induced labor combined with specific feedstuff. The model rats were randomly divided into 6 groups (n=10). These rats except those in the model group were orally administered with coix decoction (6.25 g/kg), coix oil (287.5 mg/kg), coix polysaccharide (911.3 mg/kg), coix protein (281.3 mg/kg), and coix starch (1 798.1 mg/kg) by intragastric gavage once daily. Another 10 normal rats were taken as the control group. After 14 days of intervention, the small intestine samples were collected and the gene expression of rat intestine was detected by using Agilent's whole genome expression microarray. After screening the differential genes expression (DEGs) of ion transport between model group and control group, principal component analysis (PCA) and cluster analysis were carried out for the differential genes of each group. The PCA loading and scores were also analyzed. Results There were 38 DEGs in ion transport between model rats and control rats. Bi-plot analysis of PCA on the DEGs showed that the model group was farthest from the control group, which was consistent with the clustering results of samples. After treatment with different components of coix seed, the result of each treatment group was between the model group and the control group. The analysis of DEGs showed the top 10 genes were Atox1, Slc5a4, Slc40a1, Slc5a1, Slc11a2, Cftr, Fxyd5, Cacna1i, Kcnj13 and Atp5d, and most of them were in the same category in cluster analysis. Conclusion There are apparent changes of ion transport gene in intestinal epithelial cells of DRSD rats. Different components of coix seed, especially the oil and the protein components, have strong effects on ion transport function. Solute carrier (Slc) family is the main target of spleen-strengthening and dampness-draining effects. Besides, Atox1 and Cftr also play a significant role in these effects.

Key words: Pattern of dampness retention due to spleen deficiency, strengtheing the spleen and draining dampness, Yiyiren, ion transport, solute carrier family (Scl), cystic fibrosis transmembrane conductance regulator (Cftr)

CLC Number: 

  • R285.5