主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (7): 592-598.doi: 10.3969/j.issn.1006-2157.2020.07.011

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of triptolide on IFN-γ and IL-17A expression in collagen-induced arthritic rats*

Ma Junfu1, Meng Qingliang1, Zheng Fuzeng1, Gu Huimin1, Hou Xiujuan2, Zhu Yuelan2#   

  1. 1 Henan province hospital of Traditional Chinese Medicine, Henan 450000,China;
    2 Dongfang Hospital affiliated to Beijing University of Chinese Medicine, Beijing 100078, China
  • Received:2020-01-24 Published:2020-08-11
  • Contact: Prof. Zhu Yuelan,Ph. D., Chief Physician, Doctoral Supervisor. Department of Rheumatology, Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078, China. E-mail: zhuyuelanting@163.com
  • Supported by:
    National Natural Science Foundation of China (No.81273697, No.81403370);Henan Province Special Project of Scientific Research on Traditional Chinese Medicine (No.2018ZY2041)

Abstract: Objective To study the effect of triptolide on IFN-γ and IL-17A expression in collagen-induced arthritis (CIA) rats. Methods 50 female Wistar rats were randomly divided into 5 groups (n=10 in each group): the normal group, model group, methotrexate (MTX) group and Tripterygium wilfordii glycosides tablet (TGT) group and triptolide (TPL) group. CIA rat model was established in all groups except the normal group. The normal group was fed routinely, while other groups were given by gavage with distilled water, MTX (1.78mg/kg), TGT (20mg/kg) and TPL (4mg/kg) respectively once a day for 4 weeks after CIA model was established. The weight, arthritis index (AI), foot swelling drainage volume of each groups were evaluated once a week. At week 4, ELISA assay was used to detect the IFN-γ and IL-17A levels in the peripheral blood of rats. RT-qPCR assay was used to detect the relative expression levels of IFN-γ mRNA and IL-17A mRNA in the rat knee joint. Results Compared with normal group the weight loss in each interventional group (MTX, TGT and TPL) at baseline(P< 0.05), and the AI value and the drainage volume of both feet were significantly increased (P< 0.05). After intervention, the swelling of the foot of the group reduced and AI value decreased continously. There was no significant difference in the AI and the double feet swelling drainage volume at the same time among drug-treated groups (P> 0.05). The IFN-γ and IL-17A levels in peripheral blood of model group and administration group were significantly higher compared to normal group (P< 0.05), and the level of each administration group was significantly lower than that in model group (P< 0.05).There was no significant difference between MTX, TGT and TPL group of it (P> 0.05). The IFN-γ mRNA and IL-17A mRNA relative expression of model group was significantly higher than that in normal group (P< 0.05), and the relative expression of mRNA were decreased after treatment. Except for MTX group, there was no significant difference between the other administration groups and the model group of the relative mRNA expression (P> 0.05). Conclusion Triptolide can relieve the joint swelling of CIA rats, reduce AI value and the expression of IFN-γ and IL-17A in the peripheral blood of CIA rats.

Key words: rheumatoid arthritis, triptolide, interleukin-17A, interferon-γ, collagen-induced arthritis, rats

CLC Number: 

  • R285.5