主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (8): 661-667.doi: 10.3969/j.issn.1006-2157.2020.08.008

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effect of Yiqi Yangxue Shugan Formula on glutamate-induced RGC-5 cell injury model *

Li Tiantian1, Zhou Jian2#, Yan Xiaoling2, Su Yan2, Zhou Xiaoyu1, Wei Qiping2   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Department of Ophthalmology, Dongfang Hospital, the Second Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100078, China
  • Received:2020-04-21 Online:2020-08-30 Published:2020-08-27
  • Contact: Prof. Zhou Jian, M.D., Chief Physician of Ophthalmology Department, Dongfang Hospital, the Second Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100078. E-mail:zhj9667@126.com
  • Supported by:
    National Natural Science Foundation of China (No. 81874491)

Abstract: Objective To evaluate the effect of Yiqi Yangxue Shugan Formula (also known as Qing Mang Yi Hao Formula, QMYH) on inhibiting apoptosis of RGC-5 cells by detecting the apoptosis rate in RGC-5 glutamate-induced injury model and related mechanism. Methods Staurosporine (STSN) was used to differentiate RGC-5 cells in vitro. Sodium L-glutamate was used to establish RGC-5 cell apoptosis model, and the cell culture medium containing QMYH serum was used for intervention. CCK-8 Kit was used to detect the survival rate of RGC-5 cells in each group; flow cytometry was used to detect the apoptosis rate of cells in each group; Western-blot was used to analyze the expression levels of Bcl-2/Bax signaling pathway-related proteins in RGC-5 cells; RT-PCR detected the mRNA expression levels of Bcl-2 and Bax. Results Intervention with 0.125 μmol/L STSN medium for 1 h could induce RGC-5 neuronal differentiation. 8 mmol/L sodium L-glutamate interfering for 18 h could cause 50% of RGC-5 cells injury. The medium-dose QMYH group (5% QMYH group) had the highest survival rate of RGC-5 cells (51.1%) and the lowest apoptosis rate (3.9%); compared with the low-dose and high-dose QMYH groups, the mRNA expression level of Bcl-2 in the middle-dose group was obviously up-regulated (P> 0.05); in addition, the protein Bcl-2/Bax ratio increased significantly. Conclusion The 5% QMYH serum could possibly inhibit the apoptosis of RGC-5 cells by regulating the Bcl-2/Bax signaling pathway.

Key words: Yiqi Yangxue Shugan Formula (Qing Mang Yi Hao Formula, QMYH), glutamate, RGC-5 cells, apoptosis, Bcl-2/Bax signaling pathway

CLC Number: 

  • R285.5