主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (8): 668-674.doi: 10.3969/j.issn.1006-2157.2020.08.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effect of Dahuang (rhubarb) Granule on intestinal microecological changes in rats with chronic renal failure*

Luo Xuewen1, Zeng Yuqun2, Wang Shiqi1, Zou Chuan3, Pi Chiheng1#   

  1. 1 The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Jiangxi 330006, China;
    2 Zhejiang Provincial People’s Hospital, Zhejiang 310014, China;
    3 Guangdong Hospital of Traditional Chinese Medicine, Guangdong 510120, China
  • Received:2020-02-20 Online:2020-08-30 Published:2020-08-27
  • Contact: Prof. Pi Chiheng, Chief Physician. No.445 Eight One Street, Nanchang, Jiangxi 330006. The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine. E-mail: 13907086560@163.com
  • Supported by:
    Natural Science Foundation of Guangxi Province (No.2014GXNSFBA118166), Chinese Medicine Research Project from Health Commission of Jiangxi Province (No.2016A124), Project for Academic Succession of Experience from National Traditional Chinese Medicine Expert, National Administration of Traditional Chinese Medicine

Abstract: Objective To explore the effect of Dahuang (rhubarb) Granule on intestinal microecological changes in rats with chronic renal failure. Methods The 5/6 nephrectomy model was used as chronic renal failure model, and randomly divided into sham operation group, model group, and low-dose, medium-dose, high-dose Dahuang (rhubarb) Granule groups. After 4 weeks, the body weight, blood urea nitrogen (BUN), and blood creatinine (Crea) levels of each group were measured. Four key intestinal bacteria (including E.coli, Enteroroccus, Lactobacillus and Bifidbacteria) were cultured and quantified with the real-time qPCR method. Expression of colonic proteins claudin-1 and occluding was detected with immunohistochemical method. Indole sulfate (IS) and lipopolysaccharide (LPS) levels were detected with liquid chromatography-mass spectrometry and LPS Reagent method. Results Compared with the sham operation group, in the model group renal function indexes of blood urea nitrogen (BUN) and blood creatinine (Crea) were significantly increased (P>0.05); the pathogenic bacteria E.coli and Enteroroccus were significantly increased in the model group (P>0.05), and the probiotics Bifidbacteria was significantly reduced (P>0.05); the levels of claudin-1 and occludin in the colonic epithelium were significantly reduced; the serum IS and LPS levels were significantly increased (P<0.05). Compared with the model group, in the low-dose, medium-dose and high-dose Dahuang (rhubarb) Granule groups, the levels of BUN and Crea were significantly reduced (P>0.05); in the high-dose group, the number of E.coli and Enteroroccus was reduced (P<0.05), while the amount of Bifidbacteria was significantly increased (P<0.05); the expression of claudin-1 and occludin in colonic epithelium was significantly increased in all Dahuang (rhubarb) Granule groups (P<0.05); IS and LPS levels were significantly decreased in the medium-dose and high-dose Dahuang (rhubarb) Granule groups (P<0.05). Conclusion Dahuang (rhubarb) Granule seems to regulate the intestinal microecology in rats with chronic renal failure through regulating the intestinal flora, including increasing the probiotic Bifidbacteria and reducing the pathogenic bacteria E.coli and Enteroroccus, increasing the expression of intestinal mucosal compact proteins claudin-1, occludin, and reducing intestinal toxin IS and endotoxin LPS levels, which potentially improves renal function in rats with chronic renal failure.

Key words: Dahuang (rhubarb) Granule, chronic renal failure, intestinal microecology, intestinal flora, mechanisms, rats

CLC Number: 

  • R285.5