主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (8): 675-679.doi: 10.3969/j.issn.1006-2157.2020.08.010

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Mechanism of Majie Decoction against allergic rhinitis based on network pharmacology and preliminary verification*

Wu Jinyang1,2, Zhang Boran1, Zhang Yuteng1,2, Zhang Hongchun2#   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029, China;
    2 TCM Department of Lung Disease, Respiratory Center, China-Japan Friendship Hospital, National Clinical Research Center for Respiratory Disease, Beijing 100029, China
  • Received:2020-03-03 Online:2020-08-30 Published:2020-08-27
  • Contact: Professor Zhang Hongchun, Ph.D, Chief Physician, Director of Health Care Office of China-Japan Friendship Hospital. No.2, Yinghua East Road, Chaoyang District, Beijing 100029. E-mail: 13701226664@139.com
  • Supported by:
    Traditional Chinese Medicine Inheritance and Innovation “Hundred, Thousand, and Tens of Thousands of Talents Project” (Qihuang Project) (No.2019-QTL-003)

Abstract: Objective To study the mechanism of Majie Decoction (Ephedra and White Mustard Seed Decoction) against allergic rhinitis based on network pharmacology and preliminarily verify it through in vivo experiments in mice. Methods Effective constituents of Majie Decoction (Ephedra and White Mustard Seed Decoction) were selected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP database) based on threshold of oral availability (OB) and drug-likeness (DL). Then corresponding targets of the selected constituents were obtained to form an effective constituents-targets network Targets relevant to allergic rhinitis were achieved based on Digsee database. The intersection dataset of these datasets was analyzed. Then the protein-protein reaction and its effect on the KEGG/Reactome pathway were analyzed based on String database. IL-4 signal pathway were verified by mouse model.Results 53 unduplicated effective constituents, including neobaicalein, panicolin, and 34 important targets against allergic rhinitis, including ESR1, AR, PPARG, PTGS2, DPP4, were obtained. The protein interaction network and enrichment analyses showed that targets of Majie Decoction (Ephedra and White Mustard Seed Decoction) are enriched in various inflammatory factor-related pathways. Animal experiments showed that Majie Decoction (Ephedra and White Mustard Seed Decoction) significantly reduced the levels of serum IgE, histamine and IL-4 in mice with allergic rhinitis. Conclusion The main mechanism of Majie Decoction (Ephedra and White Mustard Seed Decoction) may be relevant to regulation of multiple inflammatory factor-related pathways, especially IL-4 and IL-13.

Key words: Majie Decoction (Ephedra and White Mustard Seed Decoction), allergic rhinitis, network pharmacology, signaling pathway, verification, mouse

CLC Number: 

  • R285.5