主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (8): 680-688.doi: 10.3969/j.issn.1006-2157.2020.08.011

• Science & Technology Theme • Previous Articles     Next Articles

Effects of Houshiheisan Formula on the recovery of neurovascular function in rats with cerebral ischemia based on MRI*

Cheng Hongfa, Wang Xuan, Zhang Yawen, Zhao Hui, Wang Lei, Zou Haiyan, Zhang Qiuxia#   

  1. School of Traditional Chinese Medicine, Capital Medical University, Beijing Key Laboratory of TCM Collateral Disease Theory Research, Beijing 100069, China
  • Received:2020-03-28 Online:2020-08-30 Published:2020-08-27
  • Contact: Prof. Zhang Qiuxia, M.D., School of Traditional Chinese Medicine, Capital Medical University, No.10 Youanmen west, Fengtai District, Beijing 100069. E-mail: zqx26@163.com
  • Supported by:
    National Natural Science Foundation of China (No. 81373526, No. 81873224), Natural Science Foundation of Beijing (No. 7102014, No. 7122018)

Abstract: Objective To non-invasively assess the therapeutic effect of Houshiheisan (HSHS) Formula on the recovery of neurovascular function after cerebral ischemia in rats with application of magnetic resonance imaging (MRI). Methods Focal cerebral ischemia model was induced by permanent middle cerebral artery occlusion (pMCAO) in male sprague-Dawley (SD) rats. Then the rats were randomly divided into 5 groups: sham group, model group, HSHS low-dose group (5.25 g/kg), HSHS high-dose group (10.50 g/kg) and Ginkgo biloba extract group (28 mg/kg). Nurological score was assessed after surgery to evaluate the degree of nervous function defects. T2 weighted imaging (T2WI) and T2 relaxation imaging were used to measure the volume of cerebral infarction and the degree of brain tissue damage in rats with cerebral ischemia. Arterial spin labeled perfusion weighted imaging(ASL-PWI)and magnetic resonance angiography (MRA) were used to evaluate the hemodynamic changes of intracranial vessels. Results Compared with the model group, HSHS high-dose group and Ginkgo biloba extract group decrease neurological function score on the 5th and 7th day (P<0.05). Compared with the model group, the infarct volume reduced and the damage of white matter and gray matter was decreased, and the cerebral blood flow and signal intensity of ischemic hemisphere increased in all treatment groups (P<0.05). Compared with the sham group, the cerebral blood flow and signal intensity of ischemic hemisphere decreased in the model group (P<0.01). Conclusion HSHS seems to alleviate brain injury and improve the intracranial hemodynamics, and promote the recovery of neurovascular function.

Key words: cerebral ischemia, Houshiheisan (HSHS) Formula, Magnetic Resonance Imaging (MRI), perfusion-weighted imaging, magnetic resonance angiography, neurovascular unit, rats

CLC Number: 

  • R285.5