主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (8): 689-695.doi: 10.3969/j.issn.1006-2157.2020.08.012

• Clinical Studies • Previous Articles     Next Articles

Correlation between phlegm pattern of metabolic syndrome, fat mass and obesity-associated (FTO) gene*

Li Yuanyuan1,2, Zhang Ping1,2, Xu Chaoqiang1, Li Dan1, Chen Shujiao3,4, Gao Bizhen1,2,5#   

  1. 1 Fujian University of Traditional Chinese Medicine, Fujian 350122, China;
    2 Chinese Medicine Health Management 2011 Collaborative Innovation Center in Fujian Province, Fujian 350122, China;
    3 Fujian Key Laboratory of TCM Health Status, Fujian 350122, China;
    4 Third People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fujian 350122, China;
    5 Key Laboratory of TCM Syndrome Research in Fujian Province University, Fujian 350122, China
  • Received:2020-02-23 Online:2020-08-30 Published:2020-08-27
  • Contact: Gao Bizhen, Doctoral Supervisor. Fujian University of Traditional Chinese Medicine. No.1 Qiuyang Road, Shangjie Town, Minhou District, Fuzhou 350122. E-mail: gbz688@163.com
  • Supported by:
    National Natural Science Foundation of China (No. 81873234, No. 81273666), Natural Science Foundation of Fujian Province (No. 2018J01875)

Abstract: Objective To analyze the expression of fat mass and obesity-associated (FTO) genes in the peripheral blood of patients with phlegm pattern of metabolic syndrome (MS) and non-phlegm syndrome, and its correlation with elements and physicochemical index of phlegm pattern, so as to explore the genetic basis of MS phlegm pattern, and provide microcosmic index for the TCM diagnosis of MS phlegm pattern. Methods According to the method of “element-based pattern differentiation”, MS patients were divided into phlegm pattern group and non-phlegm pattern group (n=35), and 35 healthy people were recruited as the control group. The peripheral venous blood was collected for biochemical examination, and the expression of FTO gene mRNA and protein in human whole blood and serum were detected by real-time fluorescence quantitative PCR (probe method) and enzyme-linked immunosorbent assay (ELISA) method respectively. Results ① The relative expressions of FTO mRNA from high to low were phlegm pattern group > non-phlegm pattern group > control group, and the difference between the three groups was statistically significant (P<0.05); ② The expressions of FTO protein from high to low were control group > non-phlegm pattern group > phlegm pattern group, and the difference between the three groups was statistically significant (P<0.05); ③ The uric acid (UA) and BMI in the phlegm syndrome group were positively correlated with the score of phlegm pattern elements (P<0.05, P<0.01), and the alanine transaminase (ALT) in the non-phlegm pattern group was positively correlated with the score of phlegm pattern elements (P<0.01); ④ FTO mRNA expression was positively correlated with low-density lipoprotein (LDL-C) in the phlegm pattern group (P<0.05), positively correlated with aspartate aminotransferase (AST) in the non-phlegm pattern group (P<0.01). FTO mRNA expression was negatively correlated with Urea nitrogen (BUN) in the non-phlegm pattern group (P<0.05), and negatively correlated with LDL-C in the phlegm pattern group (P<0.05). Conclusion The high expression of FTO gene mRNA may increase the risk of phlegm pattern formation in MS. The concentrations of UA, BMI and LDL-C in the phlegm pattern group and the concentrations of ALT, AST and BUN in the non-phlegm pattern group may be related to the high expression of FTO gene. FTO gene could possibly be used as an auxiliary indicator for the diagnosis of phlegm pattern.

Key words: metabolic syndrome, phlegm pattern, FTO (Fat Mass And Obesity Associated) gene, syndrome elements, physicochemical index

CLC Number: 

  • R241.9