主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (9): 738-745.doi: 10.3969/j.issn.1006-2157.2020.09.007

• Science & Technology Theme • Previous Articles     Next Articles

Research on the differentiation method of spleen deficiency leading to damp accumulation pattern in rats with ulcerative colitis*

Jiao Wenchao, Luo Hui, Tang Jiayang, Wang Qing, Su Congping, Yu Xue, Liu Yan, Shi Bingjie, Wang Wei, Guo Shuzhen#   

  1. School of Chinese Medicine, Beijing University of Chinese Medicine,Beijing 102488, China
  • Received:2020-03-26 Published:2020-09-29
  • Contact: Prof. Guo Shuzhen, Ph. D., Doctoral Supervisor. School of Chinese Medicine, Beijing University of Chinese Medicine.Intersection of Yangguang Nandajie Street and Baiyang Donglu Road, Liangxiang Campus of Beijing University of Chinese Medicine, Liangxiang Higher Education Park, Fangshan District, Beijing China 102488. E-mail:guoshz@bucm.edu.cn
  • Supported by:
    National Key Research and Development Program(No.2017YFC1700100, No.2017YFC1700105)

Abstract: Objective To investigate the differentiation methods for the pattern of spleen deficiency leading to damp accumulation in ulcerative colitis (UC) rats from the perspectives of both the disease in Western medicine and the pattern in traditional Chinese medicine (TCM), and then establish desirable models for pharmacological evaluation of Chinese herbal medicine and basic researches in TCM pattern.Methods 40 SD rats were randomly divided into the control groups (studied for 4 weeks) and the model groups (8 weeks), 10 in each group. Immune induction together with coloclysis of 2,4, 6-trinitrobenzene sulfonic acid(TNBS) was used to induce ulcerative colitis. Macroscopic characteristics, grip strength, open field test, hair and other features of the rats were collected at multiple time points. The colon samples were taken on the 28th and 56th days after coloclysis and the pathological changes were analyzed using HE stained sections. The corresponding criteria for pattern differentiation of rats were established based on common clinical diagnostic criteria of ulcerative colitis.Results Three days after coloclysis, rats in the model groups manifested relatively lusterless and frizzy hair, anorexia, significant weight loss, diarrhea, bloody stool, and increased resistance against grasping but normal vitality, responsiveness, autonomous activity and general state. From the fourth day to the end of the second week, compared with the control group, no significant differences were observed in the model rats except loose and sticky stool. In the third and fourth weeks, the model rats excreted loose stools, ate less and showed decreased grip strength (P<0.05) and lusterless and frizzy hair. Compared with the control groups, they also showed decreased vitality, resistance against grasping and responsiveness and UC was diagnosed based on their colonic pathological test results. From the fifth week to the eighth week, all the parameters of the model rats returned to normal gradually.Conclusion During the third and fourth weeks after coloclysis, the model rats could meet the diagnostic criteria of ulcerative colitis with spleen deficiency leading to damp accumulation pattern.

Key words: ulcerative colitis, rat, pattern evaluation, spleen deficiency leading to damp accumulation pattern

CLC Number: 

  • R241.6