主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (9): 754-761.doi: 10.3969/j.issn.1006-2157.2020.09.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Louqin Zhisou Youhua Fang on improving antibiotic permeability through Pseudomonas aeruginosa biofilm by correcting inflammatory immune imbalance*

Li Jie1, Wu Tong1, Yu Ziyang1, Liu Liting1, Huang Mingming1, Zhao Cuiyun1, Meng Zixia1, Peng Guiying2#   

  1. 1 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700 China;
    2 School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488,China
  • Received:2020-03-16 Published:2020-09-29
  • Contact: Associate Prof. Peng Guiying, Doctor of Science, Master’s Supervisor. Beijing University of Chinese Medicine. Intersection of Yangguang Nandajie Street and Baiyang Donglu Road, Liangxiang Campus of Beijing University of Chinese Medicine, Liangxiang Higher Education Park, Fangshan District, Beijing 102488. E-mail: Pengguiying@bucm.edu.cn
  • Supported by:
    National Natural Science Foundation of China for Young Scientists (No. 81202681)

Abstract: Objective To explore the immune protective effects of Louqin Zhisou Youhua Fang (Snakegourd-fruit Scutellaria-root Cough-stopping Formula with Modifications, LQZSYHF) on infection with Pseudomonas aeruginosa. Methods The effects of LQZSYHF on improving the penetration of levofloxacin through the PA mature biofilm (BF) were studied via in vitro experiment. Its effects on regulating inflammatory immune imbalance were explored through observing the levels of TNF-α and IFN-γ in the lung tissues of PA-infected rats via in vivo experiment. Results In vitro, both LQZSYHF and ambroxol assisted levofloxacin in penetrating PA mature biofilms. In vivo, both the Western drug and Chinese herbal medicine significantly promoted the elimination of lung bacteria and reduced the pathological damage to lung tissue. TNF-α level in lung tissue was significantly reduced and IFN-γ level was greatly increased in the Chinese herbal medicine (CHM) group compared with the model group (P<0.001). TNF-α level in lung tissue was greatly significantly reduced (P<0.001) and IFN-γ level was slightly increased without statistical significance in the Western drug (WG) group compared with the model group. TNF-α level in lung tissue was significantly reduced and IFN-γ level was greatly increased in the CHM group compared with WD group (P <0.001). Conclusion LQZSYHF can help antibiotics pass through mature biofilms in vitro. Its mechanism might be that it can correct the immune imbalance in PA-infected rats, by which it accelerates the elimination of lung bacteria and reduces lung pathological damage, thereby reducing inflammatory response, and it enhances body resistance against PA biofilm infection, thereby indirectly eliminating bacteria. Compared with ambroxol, its advantages may lie in improving immunity, and its underlying mechanism warrants further study.

Key words: Louqin Zhisou Youhua Fang, inflammatory immune imbalance, antibiotic, Pseudomonas aeruginosa, biofilm, rats

CLC Number: 

  • R285.5