主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (9): 762-768.doi: 10.3969/j.issn.1006-2157.2020.09.010

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Experimental study on the effect of Qishen Yiqi Dripping Pill on myocardial ischemia-reperfusion injury via PI3K/AKT signaling pathway*

He Guixin1, Xiao Ting2, Qin Weibin1, Mo Xiaoyun1, Lin Lin1, Ren Jiayi2, Shen Yongyan2, Yu Liyan2, Feng Yufei2, Zheng Guokun2   

  1. 1 The First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi 530022, China;
    2 Guangxi University of Chinese Medicine, Guangxi 530299, China
  • Received:2020-03-13 Published:2020-09-29
  • Contact: Prof. He Guixin, M.D. Chief Physician, Doctoral Supervisor. The First Affiliated Hospital of Guangxi University of Chinese Medicine. No. 89-9 Dongge Road, Nanning 530022. E-mail:he_guixin@163.com
  • Supported by:
    National Natural Science Foundation of China (No. 81960861, No. 81460712); Guangxi Key Scientific Research and Development Project (No. AB19110006)

Abstract: Objective To observe the protective effect of Qishen Yiqi (Astragalus Root and Danshen Root Qi-boosting) Dripping Pills (QSYQDP) on myocardial ischemia-reperfusion injury and its correlation with the activity of PI3K/AKT pathway. Methods Models of myocardial ischemia-reperfusion injury were established by ligating the left anterior descending branch of coronary artery in miniature pigs. Sixty healthy Bama miniature pigs were randomly divided into 6 groups (n=10): sham-operated group, model group, low-dose QSYQDP group (0.125 g/kg), mid-dose QSYQDP group (0.25 g/kg), high-dose QSYQDP group (0.3 g/kg), and QSYQDP plus wortmannin group. QSYQDP plus wortmannin group was given 20 mg/g PI3K/AKT signaling pathway blocker wortmannin and QSYQDP 300 kg/L via gavage. All the groups were treated continuously for 4 weeks. The levels of serum CK, CK-MB, AST, CRP and cTnT were detected by ELISA, and the levels of p-AKT and AKT were assessed by Western blotting. During the treatment, the second lead ECG was continuously monitored, and the frequency of ventricular fibrillation and the amplitude of ST segment elevation were recorded. Results ECG results showed that the incidences of ventricular fibrillation in the QSYQDP groups were significantly lower than that in the model group (P<0.05). Levels of CK, CK-MB and cTnT in the model group and low-dose QSYQDP group were significantly higher than those in the sham-operated group (P<0.05). Compared with the model group, the contents of AST, cTnT and CRP in the low-, mid- and high-dose QSYQDP groups were significantly lower. The cTnT and CRP levels in the high-dose QSYQDP group were lower than those in the low-dose QSYQDP group with statistically significant difference (P<0.05). Compared with the model group, the expression levels of p-AKT in the low-, mid- and high-dose QSYQDP groups increased (P<0.05), but there was no significant change in total AKT protein levels in any group (P>0.05). Conclusion QSYQDP can improve in a dose-dependant manner the cardiac function, reduce the incidence of ventricular fibrillation and activate the PI3K/AKT signaling pathway in miniature pigs with myocardial ischemia, so as to reduce the myocardial ischemia-reperfusion injury and protect cardiomyocytes.

Key words: Qishen Yiqi Dripping Pills, PI3K/AKT signaling pathway, myocardial ischemia-reperfusion injury, miniature pigs

CLC Number: 

  • R285.5