主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (10): 834-840.doi: 10.3969/j.issn.1006-2157.2020.10.007

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Compound Zhebei Extract (CZBE) on the expression of apoptotic protein and xenograft tumors of human colon cancer oxaliplatin-resistant cells (HCT-116/L-OHP) in nude mice: an experimental study*

Lyu Peng1,2, Dong Qing1, Liu Yu1, Li Ruibai1, Fan Qiuyue1, Chen Xinyi1, Hou Li1#   

  1. 1 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    2 Central Hospital, Enshi Tujia and Miao Nationality Autonomic Region, Hubei 445000, China
  • Received:2019-12-20 Online:2020-10-30 Published:2020-11-04
  • Contact: Prof. Hou Li, Ph.D, Doctoral supervisor, Dongzhimen Hospital affiliated to Beijing University of traditional Chinese Medicine, No.5, Haiyuncang Road, Dongcheng District, Beijing 100700, E-mail:houli1203@126.com
  • Supported by:
    “Ten Diseases and Ten Drugs Research and Development” project of Beijing Science and Technology Plan (No.Z151100003815027)Ethical review: Experimental Animal Welfare Ethics Committee of Institute of Traditional Chinese Medicine, Chinese Academy of Sciences (No.20181003)

Abstract: Objective To observe the inhibitory effect of Compound Zhebei Extract(CZBE) on HCT-116/L-OHP transplanted tumors in nude mice and the expression of apoptotic proteins related to transplanted tumors. Methods HCT-116/L-OHP cells were inoculated into the axilla of BALB/c nude mice to establish L-OHP-resistant transplantation model of colon cancer. After the successful establishment of the model, the transplanted mice were divided into five groups: control group, L-OHP group (3 mg/kg), high-dose CZBE (10 g/kg) combined with L-OHP group, mid-dose CZBE (5 g/kg) combined with L-OHP group, and low-dose CZBE (2.5 g/kg) combined with L-OHP group. Method of administration: CZBE, intragastric administration, once a day; L-OHP, 3 mg/kg, intraperitoneal injection, once every other day for 2 consecutive weeks. At the end of the experiment, the mice were sacrificed and weighed, and then the tumors were removed completely and weighed to calculate the tumor inhibition rate. The tumors were sliced and photographed. Then the effects of CZBE on apoptotic protein expression of HCT-116/L-OHP transplanted tumors were analyzed by using ImageProPlus 7.0 and SPSS21 software. Results CZBE combined with L-OHP could effectively inhibit the growth of HCT-116/L-OHP transplanted tumors in nude mice. The inhibition rate of high-dose CZBE combined with L-OHP group was 45.031%, which was significantly different from that of L-OHP group (P<0.05). The inhibition rate of mid-dose CZBE combined with L-OHP group was 37.669%. The inhibition rate of low-dose CZBE combined with L-OHP group was 32.147%. The inhibition rate of L-OHP group was 27.239% in the positive control group, and the survival time of transplanted nude mice was prolonged. Compared with L-OHP group, IOD/Area of Bcl-2 cells in low-dose CZBE combined with L-OHP group decreased(P<0.05), while IOD/Area of Bcl-2 cells in mid-dose and high-dose CZBE combined with L-OHP group decreased significantly (P<0.01). IOD/Area values of Bax in mid-dose CZBE combined with L-OHP group increased (P<0.05), while IOD/Area values of Bax in high-dose CZBE combined with L-OHP group increased significantly (P<0.01). Conclusion CZBE combined with L-OHP can improve the tumor inhibition rate of L-OHP-resistant human colon cancer cell lines and prolong the survival time of Oxaliplatin-resistant nude mice. The mechanism may be to reverse multidrug resistance and promote apoptosis of tumor cells by regulating Bax/Bcl-2 apoptotic pathway.

Key words: Compound Zhebei Extract, colon cancer, L-OHP, multidrug resistance, HCT-116/L-OHP cell, mice

CLC Number: 

  • R285.5