主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (11): 903-909.doi: 10.3969/j.issn.1006-2157.2020.11.004

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Mechanism of paeoniflorin on LPS-induced human chondrocyte inflammation and degeneration based on NF-kB signaling pathway*

Chen Pu, Ruan Anmin, Zhou Jun, Zhang Xiaozhe, Ma Yufeng, Wang Qingfu#   

  1. The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China
  • Received:2020-04-30 Online:2020-11-30 Published:2020-12-08
  • Contact: Wang Qingfu,Chief Physician,Doctoral Supervisor.The Third Affiliated Hospital of Beijing University of Chinese Medicine,51 XiaoGuan Street,AnDingMen,ChaoYang District,Beijing 100029.E-mail:13910052566@163.com

Abstract: Objective To observe the effects and explore the mechanism of paeoniflorin (PF) on the inflammation and degeneration of LPS-induced human chondrocytes.Methods Discarded cartilage tissues from primary knee osteoarthritis (KOA) patients undergoing total knee arthroplasty at The Third Affiliated Hospital of Beijing University of Chinese Medicine from April 2019 to October 2019 were collected and digested into chondrocytes using pancreatin and collagenase.The effect of different concentrations of PF on the viability of chondrocytes was detected using the CCK-8 assay,and appropriate concentrations were selected for subsequent experiments.Chondrocytes were randomly divided into five groups,namely the blank group,the model group,and the low,medium,and high concentration PF intervention groups.Except for the blank group,chondrocytes in the other four groups were induced by LPS to simulate an arthritis environment.In the low,medium,and high concentration PF intervention groups,10,20,and 50 μMol/L PF were given respectively.The expressions of IL-1β,IL-6,TNF-α,MMP13 and ADAMTS5 in each group were detected by qPCR assay;and the expression levels of NF-kB p65,IKB and IkK were detected by Western-blot.Results The results of CCK-8 assay showed that 10,20,and 50 μMol/L PF could significantly increase the level of proliferation and viability of human chondrocyte;while excessively high concentration (200 μM) PF could inhibit chondrocyte viability.Further qPCR results showed that PF could significantly inhibit the expression levels of inflammatory factors IL-1β,IL-6,TNF-α and cartilage degradation factors MMP13 and ADAMTS5 in human chondrocytes after LPS intervention,compared with the model group (P<0.05);WB results showed that PF could significantly inhibit NF-kB p65 and IkK expresion and promotes IkB expression (P<0.05).Conclusion Paeoniflorin seems to inhibit LPS-induced human chondrocyte inflammation and delay cartilage degeneration by inhibiting the activation of NF-kB signaling pathway.

Key words: paeoniflorin, osteoarthritis, cartilage degeneration, inflammation, NF-kB signaling pathway

CLC Number: 

  • R285.5