主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (11): 918-926.doi: 10.3969/j.issn.1006-2157.2020.11.006

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Regulating effect and mechanism of Zuogui Jiangtang Jieyu Formulation on linkage damage of hippocampal neurovascular unit in rats with diabetes-related depression*

Liu Jian1, Liu Lin1, Yang Hui1, Han Yuanshan1, Wang Yuhong2,3#   

  1. 1 The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;
    2 Hunan Province-Ministry Jointly Built National Key Laboratory Cultivation Base for Chinese Materia Medica Powder and Innovative Drug Research,Changsha 410208,China;
    3 Institute of Innovation and Applied Research,Hunan University of Chinese Medicine,Changsha 410208,China
  • Received:2020-04-30 Online:2020-11-30 Published:2020-12-08
  • Contact: Wang Yuhong,M.D.,Doctoral Supervisor.Institute of Innovation and Applied Research,Hunan University of Chinese Medicine,No.300,Xueshi Road,Hanpu Science and Education Park,Yuelu District,Changsha 410208.E-mail:wyh107@ 126.com

Abstract: Objective To investigate the regulating effect and mechanism of Zuogui Jiangtang Jieyu Formulation (ZGF) on linkage damage of hippocampal neurovascular unit (NVU) in diabetes-related depression.Methods Hippocampal neurons,astrocytes and brain microvascular endothelial cells from SD rats were primitively isolated,purified and cultured,and immunofluorescence staining was used to identify cells.The DD model of hippocampal NVU was established with high dose of glucose and cortisone after hippocampal NVU co-culture system was built by Transwell in vitro.The cultured cells were randomly divided into normal group,model group,blank serum group,and ZGF-containing serum group.After molding and administration for 18 hours,transendothelial electrical resistance(TEER)and leakage liquid height for 4h leakage test (LLH) were detected.The content of secretory protein LIF for Bm and TGF-β and GDNF for As was detected by enzyme linked immunosorbent assay (ELISA).The expression of structural protein claudin-1,occludin for Bm and GFAP,CX43 for As was detected by immunofluorescent staining and high content analysis.Cell survival rate and apoptosis of hippocampal neuron was detected by CCK-8 assay and Tunel staining respectively.Results Compared with the normal group,in both model group and blank serum group,the TEER value was significantly reduced,the LLH was significantly increased,the content of protein LIF,TGF-β and GDNF were significantly reduced,and the expression of structural proteins claudin-1,occludin,GFAP and CX43 was down-regulated,the survival rate of hippocampal neuron cells decreased,and cell apoptosis increased significantly.Compared with the model group,in the ZGF group,the TEER value was significantly increased,the LLH was significantly decreased,the expression of LIF,TGF-β,GDNF,claudin-1,occludin,GFAP and CX43 was obviously up-regulated,the survival rate of hippocampal neuron cells increased,and cell apoptosis decreased significantly.Conclusion ZGF seems to have a significant regulating effect on linkage damage of hippocampal NVU in diabetes-related depression,and its mechanism might be related to the improvement the barrier function,secretion function and basic structural protein of hippocampal NVU.

Key words: Zuogui Jiangtang Jieyu Formulation (ZGF), diabetes-related depression, neurovascular unit (NVU), linkage damage, apoptosis, rats

CLC Number: 

  • R285.5