主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (11): 927-934.doi: 10.3969/j.issn.1006-2157.2020.11.007

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Liuwei Dihuang Decoction on the expression of HIF-1α, Twist and E-cadherin in renal tissue of 5/6 nephrectomized rats*

Dong Xiang1, Hu Shuang1, Wang Xi1, Yin Xin1, Zhang Haiying1, Tang Qun2#   

  1. 1 Graduate School,Hunan University of Chinese Medicine,Changsha 410208,China;
    2 Department of Pathology,Hunan University of Chinese Medicine,Changsha 410208,China
  • Received:2020-05-07 Online:2020-11-30 Published:2020-12-08
  • Contact: Tang Qun,Ph.D,Master’s Supervisor.Department of Pathology,Hunan University of Chinese Medicine,300 Xueshi Road,Hanpu Science and Education Park,Changsha 410208.E-mail:tangqun460@126.com

Abstract: Objective To observe the effect of Liuwei Dihuang Decoction on the expression of HIF-1α,Twist and E-cadherin in 5/6 nephrectomy rats,and explore the molecular mechanism of Liuwei Dihuang Decoction in delaying renal fibrosis.Methods 60 male SD rats were randomly divided into the control group (n=10),sham operation group (n=10) and operation group (n=40).The model of 5/6 nephrectomy rats was established in the operation group.After successful modeling,the operation group (one dead) was randomly divided into model group,Liuwei Dihuang Decoction group,and enalapril group (n=13).The Liuwei Dihuang Decoction group and the enalapril group were gavaged with the decoction 6.75 g/kg and enalapril 10 mg/kg respectively.The control group,the sham operation group and the model group were gavaged with saline 10 g/kg.Eight weeks after the model was established,the renal function indexes of each group were measured.HE staining was used to observe the pathological changes of kidney tissues.Masson staining was used to observe collagen deposition and measure the percentage of collagen area.Immunohistochemical method and qPCR method were used to detect the protein level and gene expression of HIF-1α,Twist and E-cadherin in renal tissue.Results 24 hours urinary protein,blood urea nitrogen and blood creatinine were significantly increased in the model group (P<0.05),and decreased in the Liuwei Dihuang Decoction group compared with the control group (P<0.05).A large amount of collagen fiber deposition and obvious fibrillation were observed in the model group,while only a small amount of collagen fiber deposition and decreased fibrillation were observed in the Liuwei Dihuang Decoction group and the enalapril group.Compared with the sham operation group,the HIF-1α and Twist protein levels and mRNA expression in the kidney tissue were significantly increased (P<0.05),and the E-cadherin protein and mRNA expression were significantly reduced in the model group (P<0.05).Compared with the model group,after 8 weeks’ treatment,in the Liuwei Dihuang Decoction group and the enalapril group,there were significantly lowered levels of HIF-1α,Twist protein and mRNA expression (P<0.05),and significantly increased E-cadherin protein levels And mRNA expression (P<0.05).Conclusion Liuwei Dihuang Decoction seems to reduce the expression of HIF-1α to reduce the expression of Twist and promote the expression of E-cadherin,thus delaying renal fibrillation.The mechanism might be related to the inhibition of renal tubular epithelial-mesenchymal transformation.

Key words: 5/6 nephrectomy, Liuwei Dihuang Decoction, renal tubular epithelial-mesenchymal transition, hypoxia inducible factor-1α, renal fibrosis, rats

CLC Number: 

  • R285.5