主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (11): 935-940.doi: 10.3969/j.issn.1006-2157.2020.11.008

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Shenfuhuang Formula on cognitive function and synaptic plasticity in septic mice*

Wang Xuerui, Guo Yuhong, Zhao Jingxia, Xu Xiaolong, Liu Qingquan#   

  1. Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China
  • Received:2020-05-18 Online:2020-11-30 Published:2020-12-08
  • Contact: Liu Qingquan,Chief Physician,Doctoral Supervisor.Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,No.23 Meishuguan Bavk Street,Dongcheng District,Beijing 100010.E-mail:liuqingquan_2003@126.com

Abstract: Objective To investigate the effect of Shenfuhuang Formula on spatial learning and memory,anxiety state,and hippocampal CA1 synaptic plasticity in sepsis mice.Methods C57BL/6 mice were randomly divided into control group,model group and Shenfuhuang group.The sepsis model was established with cecal ligation and perforation (CLP) in the latter two groups.The Shenfuhuang group was gavaged with 2.5 g/kg Shenfuhuang Decoction,twice daily,with a total volume of 0.5 mL;the model group and the control group were given 0.5 mL normal saline twice daily.Morris water maze test was used to evaluate the spatial learning and memory ability.The open field test was used to evaluate the exploration ability and anxiety state.The long-term potentiation of hippocampal CA1 area was also recorded in vivo by neurophysiological technology.Results Compared with the control group,in model group the escape latency and the time spent in target quadrant of Morris water maze were significantly reduced (P<0.05);the number of crossing,rearing and center duration of the open field test were significantly reduced (P<0.05);and the slope of the field post-synaptic potential were significantly reduced (P<0.05).Compared with the model group,in Shenfuhuang group the escape latency and the time spent in target quadrant were significantly increased (P<0.05);the number of crossing,rearing and center duration of the open field test were significantly increased (P<0.05);and the slope of the field post-synaptic potential were significantly increased (P<0.05).Conclusion Shenfuhuang Formula seems to improve the cognitive impairment and behavioral ability of CLP mice to a certain degree,and its mechanism may be related to the improvement of synaptic plasticity in the hippocampus.

Key words: sepsis, Shenfuhuang Formula, cognitive function, synaptic plasticity, mouse

CLC Number: 

  • R285.5