主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (11): 941-950.doi: 10.3969/j.issn.1006-2157.2020.11.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effect of Erchen Decoction on protein expression of Bcl-2 and Bax in ovarian tissue of rats with polycystic ovary syndrome*

Zhang Ping1,2, Wang Weibin1,3, Shao Yanfei4, Chen Jinming1,3, Yu Jie1,3, Gao Bizhen1,2#   

  1. 1 School of Traditional Chinese Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;
    2 Fujian Provincial Key Laboratory for Research on TCM Pattern,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;
    3 Key Laboratory of TCM Health Differentiation in Fujian Province,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;
    4 Ruijin Hospital affiliated to Shanghai Jiao Tong University,Shanghai 200025,China
  • Received:2020-05-14 Online:2020-11-30 Published:2020-12-08
  • Contact: Gao Bizhen,Doctoral Supervisor,Fujian University of Traditional Chinese Medicine,No.1 Qiuyang Road,Minhou District,Fuzhou 350122.E-mail:gbz6886@163.com

Abstract: Objective To observe the effect of Erchen Decoction on the expression of B lymphocyte-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in ovarian tissue of polycystic ovary syndrome (PCOS) rats induced by DHEA,and explore the possible molecular mechanism of Erchen Decoction in the treatment of PCOS.Methods 36 female SPF SD rats were randomly divided into normal group (n=10) and model group (n=26).Rats in the normal group were injected subcutaneously with 0.2 mL of soybean oil every day.Rats in the model group were injected with the same amount of DHEA subcutaneously at the same site every day for 20 days.By observing the rats’vaginal exfoliated cells,sex hormone levels and ovarian morphology and structure,it was judged whether the model had been successfully established.The model rats were divided into model group,Erchen Decoction group (4.35 g·kg-1·d-1),metformin group (0.3 g·kg-1·d-1) (n=8 in each group).The Erchen Decoction group and metformin group were gavaged with the same dose of injection or decoction,while the model group and normal group were given with equal amount of sterilized drinking water.The administration volume for each group was 10 mL·kg-1·d-1,and the intervention continued for 4 weeks.After the last administration,serum and ovarian specimens of each group were collected.Enzyme-linked immunosorbent assay (ELISA) was used to detect hormone level of serum testosterone (T),luteinizing hormone (LH),follicle stimulating hormone (FSH) and estradiol (E2).Hematoxylin-eosin staining (HE) was used to observe the morphological changes of ovaries in each group.Terminal deoxynucleotide transferase-mediated dUTP notch end labeling assay (TUNEL) was used to detect ovarian granulosa cell apoptotic index.Immunohistochemistry was used to detect the expression of Bax and Bcl-2 proteins.Results (1)On the 21st day of modeling,compared with the normal group,the weight of model rats and the levels of serum T,LH and E2 increased significantly (P<0.05),but with no significant changes in FSH level (P>0.05).Observed with the ovarian tissue microscope,the granulosa cells in the normal group were multi-layered and tightly arranged;while the granulosa cell layer of the model module became thinner,sparsely arranged,and the follicles expanded to be cystic.(2)After treatment with Erchen Decoction,compared with the model group,the serum T and LH levels of the rats in the Erchen Decoction group decreased significantly (P<0.05),and the levels of E2 and FSH increased significantly (P<0.05).The granulosa cell layer of the model group thickened and the number of cystic follicles decreased.The apoptotic index of ovarian granulosa cells decreased,but with no statistically significant difference.The expression level of ovarian tissue Bcl-2 protein increased (P<0.05) ,while the level of Bax protein decreased (P<0.05).Conclusion The effect of Erchen Decoction on PCOS may be achieved by promoting the expression of Bcl-2 protein in ovarian tissue and down-regulating the expression of Bax protein,thereby inhibiting the apoptosis of ovarian granulosa cells.

Key words: polycystic ovary syndrome ( PCOS), Erchen Decoction, Bax, Bel-2, Bel-2, rats

CLC Number: 

  • R285.5