主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (12): 1027-1033.doi: 10.3969/j.issn.1006-2157.2020.12.009

• Chinese Medicinal Pharmocology • Previous Articles     Next Articles

Protective effects of timosaponin B-II on mitochondria of SH-SY5Y cell model of MPP+-induced Parkinson's disease*

Zhang Jinkun, Zhou Mengqi, Ma Haojie, Zhang Yuxin, Cheng Cuicui, Yang Luping, Gai Cong, Gao Yushan, Sun Hongmei#   

  1. School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2020-06-17 Online:2020-12-30 Published:2021-01-05
  • Contact: Prof. Sun Hongmei, Ph. D.,Doctoral Supervisor. School of Chinese Medicine,Beijing University of Chinese Medicine. No.11 Beisanhuan Donglu Road, Chaoyang District, Beijing 100029. E-mail:hm-sun@163.com
  • Supported by:
    National Natural Science Foundation of China (No. 81774110)

Abstract: Objective To explore the protective effects of the effective component of timosaponin B-II (TB-II) on mitochondria in Parkinson’s disease (PD) model induced by 1-methyl-4-phenyl-pyridine (MPP+, ). Methods SH-SY5Y, a human dopaminergic neuroblastoma, was selected and treated with MPP+, to establish a PD cell model. TB-II at concentrations of 0.3, 1.0, and 3.0 μmol/L were used for intervention. CCK-8 was used to detect cell survival rate of different groups, flow cytometry cell apoptosis rate and reactive oxygen species (ROS) level, and laser scanning confocal microscope mitochondrial activity. Image J software was used to evaluate mitochondrial morphology factor, average length of network branch and number of network branches. JC-1 was employed to detect mitochondrial membrane potential, and luciferase cellular ATP level. Results Compared with the model group, the cell survival rate of the TB-II low, medium, and high concentration groups were significantly increased (P<0.05, P<0.01), the apoptosis rate was significantly reduced, the mitochondrial activity, the morphological factors, the average length and the number of network branchesantagonizing the decreace of membrane potential, the relative ATP level, the cellular ROS level were increased significantly (P<0.01). In addition, it was observed that the TB-II medium concentration group performed best in antagonizing the decrease of membrane potential and increasing ATP levels. Conclusion TB-II can limit MPP+, induced damage to SH-SY5Y cell mitochondrial function, antagonize the reduction of membrane potential, increase ATP production, reduce ROS production, repair mitochondrial network morphology, thereby suppressing apoptosis and relieving PD.

Key words: TB-II, SH-SY5Y cells, Parkinson's disease, mitochondria

CLC Number: 

  • R285.5