主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2021, Vol. 44 ›› Issue (1): 39-44.doi: 10.3969/j.issn.1006-2157.2021.01.007

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Tongyan spray Formula on motility related neurotransmitters in hypoglossal nuclei of rats with dysphagia*

Jian Guoxuejiao, Li Chunrui, Hu Suqin, Guo Jian#   

  1. School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China
  • Received:2020-07-22 Online:2021-01-30 Published:2021-01-18
  • Contact: Prof.Guo Jian,Ph.D.,Doctoral Supervisor.School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Northeast Corner of the Iwtersction of Yangguang South Street and Baiyang East Road,Fangshan District,Beijing 102488.E-mail:guojian323@sina.com.
  • Supported by:
    National Natural Science Foundation of China(No. 81473623);Science development Foundation of Beijing University of Chinese Medicine(2019ZXFZJJ021)

Abstract: Objective To explore the mechanism of relieving dysphagia by using Tongyan spray formula.Methods Adult Wistar male rats were randomly divided into sham operation group,model group and Tongyan spray formula group.Rats in the sham operation group were only Operated to isolate hypoglossal nerve,and rats in the model group and the Tongyan spray formula group were Operated to isolate hypoglossal nerve and then the vulnus was compressed with hemostatic forceps for 10s to make a model.From the second day after modeling,Tongyan spray formula group was given drugs on the tongue (0.3 mL per time,once a day),and the sham operation group and model group were given the same dose of saline.After administration for 7 days,rat medulla oblongata tissue was collected,Nissl body was detected with Nissl staining,and serotonin (5-HT),Acetylcholinesterase (AChE),p38,Neuronal nitric oxide synthase (nNOS) were detected by using immunohistochemical method.Results Compared with the sham operation group,the expressions of Nissl body,5-HT and AChE were decreased,and p38 and nNOS were increased (P<0.05) in the model group,which referred to the success of modeling.After administration,the expressions of Nissl body,5-HT and AChE were increased (P<0.05),but p38 and nNOS were decreased (P<0.05).There was also a significant difference in the expression of neurotransmitters between the sham operation and Tongyan spray formula group.Conclusion Tongyan spray may relieve the dysphagia due to hypoglossal nerve clamp injury,and its possible mechanism seems to be related to the inhibition of p38 and NO and the increasing of 5-HT and ACh.

Key words: Tongyan spray formula, dysphagia, hypoglossal nerve injury, hypoglossal nuclei, rats

CLC Number: 

  • R285.5