主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2021, Vol. 44 ›› Issue (1): 45-53.doi: 10.3969/j.issn.1006-2157.2021.01.008

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effect of Shenlin Baizhu San on protein and mRNA expression of TLR2, MyD88, COX-2 in ulcerative colitis rats with spleen deficiency and dampness stagnation pattern*

Li Zihui1,2, Cai Ronglin3, Sun Juan1,2, Wen Yinjun4, Wang Rui4   

  1. 1 School of Traditional Chinese Medicine,Anhui University of Chinese Medicine,Anhui 230012,China;
    2 Key Laboratory of Xin An Medicine,Ministry of Education,Anhui University of Chinese Medicine,Anhui 230038,China;
    3 School of Acupuncture and Moxibustion,,Anhui University of Chinese Medicine,Anhui 230012,China;
    4 Graduate School,Anhui University of Chinese Medicine,Anhui 230012,China
  • Received:2020-04-27 Online:2021-01-30 Published:2021-01-18
  • Contact: Prof.Cai Ronglin,Ph.D.,School of Acupuncture and Moxibustion,Anhui University of Chinese Medicine.No.350 Longzihu Road,Xinzhan District,Hefei 230012.E-mail:ronglincai@163.com.
  • Supported by:
    National Natural Science Foundation of China (No. 81302901),Project of Natural Science Foundation of Anhui Province (No. 2008085MH298),Key Projects of Natural Science Research in Universities of Anhui Province (No. KJ2018A0271)

Abstract: Objective To observe the effect of Shenling Baizhu San (Ginseng-Poria-Atractylodes Powder,SLBZS) on the expression of Toll like receptor 2 (TLR2),myeloid differentiation factor 88 (MyD88) and cyclooxygenase-2 (COX-2) in colonic tissue of rats with ulcerative colitis (UC) of spleen deficiency and dampness stagnation pattern,and to explore the mechanism of SLBZS in relieving UC.Methods The model of UC with spleen deficiency and dampness stagnation pattern was reproduced by TNBS/ethanol enema combined with environment at and dietarg intervention.Rats were randomly divided into five groups:normal group,model group,SLBZS group,SLBZS+ TLR2 agonist (Pam3csk4) group,and SLBZS+ TLR2 antagonist (T2.5) group (n=12 in each group for continuous intervention of 14 days).The levels of TNF-α,IL-6 and IL-1β in the serum were detected by ELISA,the expression of TLR2,MyD88 and COX-2 protein in colon tissue was measured with immunohistochemical method and Western Blot Assay,and the expression of TLR2 mRNA,MyD88 mRNA and COX-2 mRNA in colon tissue of rats in each group was measured by using real time PCR.Results The levels of TNF-α,IL-6 and IL-1β in the serum of UC rats with spleen deficiency and dampness stagnation pattern were increased (P<0.05),and the expressions of TLR2,MyD88,COX-2 and TLR2 mRNA,MyD88 mRNA and COX-2 mRNA in the colon tissue were increased (P<0.01).After the treatment of SLBZS,the levels of TNF-α,IL-6 and IL-1β in the serum of UC rats were decreased,and the expression of TLR2,MyD88,COX-2 protein and mRNA were decreased,compared with the model group (P<0.01).Compared with SLBZS group,there was no difference in the levels of TNF-α,IL-6 and IL-1β in the serum,and the expression of TLR2,MyD88,COX-2 protein and mRNA in colon tissue,in SLBZS+TLR2 antagonist group (P>0.05).Compared with SLBZS group,the levels of TNF-α,IL-6 and IL-1β in the serum,and the expression of TLR2,MyD88,COX-2 protein and mRNA in colon tissue of SLBZS+TLR2 agonist group were higher (P<0.01).Conclusion SLBZS may significantly decrease the expression of TLR2,MyD88,COX-2 protein and mRNA in colon tissue of UC rats with spleen deficiency and dampness stagnation pattern,and reduce the release of inflammatory factors.It is one of the important mechanisms of SLBZS in the treatment of UC by negatively regulating TLR2/MyD88 signal pathway to reduce intestinal inflammation.

Key words: Shenling Baizhu Powder, ulcerative colitis, spleen deficiency and dampness stagnation pattern, toll-like receptor 2, myeloid differentiation factor 88, rats

CLC Number: 

  • R285.5