主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2021, Vol. 44 ›› Issue (1): 54-59.doi: 10.3969/j.issn.1006-2157.2021.01.009

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Kangxian Yixin Formula improved cardiac function in rats with dilated cardiomyopathy via AMPK/mTOR pathway*

Chang Hongbo1, Wang Zhentao2#, Liu Shunyu1, Ren Xueping1, Wu Hong1   

  1. 1 Henan University of Chinese Medicine Cell Image Lab,Henan 450046,China;
    2 Department of Cardiology,Henan Province Hospital of Traditional Chinese Medicine,Henan 450002,China
  • Received:2020-07-09 Online:2021-01-30 Published:2021-01-18
  • Contact: Dr.Wang Zhentao,Chief Physician.No.6 Dongfeng Road,Zhengzhou,Henan Province Hospital of TCM,Henan 450002.E-mail:hnzy281913220 @126.com.
  • Supported by:
    National Natural Science Foundation of China(No.81573920)

Abstract: Objective To investigate the effect of Kangxian Yixin Formula (KXYXF) on cardiac function and adenosine 5‘-monophosphate -activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway of cardiomyocytes in rats with dilated cardiomyopathy (DCM).Methods 60 Wistar rats were randomly divided into normal group,model group,KXYXF group and captopril group.Rat DCM model was established by administering furazolidone solution.After 8-week intervention,the changes of left ventricular end systolic diameter (LVESD),left ventricular end diastolic diameter(LVEDd),fraction shortening (FS),left ventricular ejection fraction(LVEF) were measured by using echocardiography;the pathological changes of heart tissue were evaluated with HE staining;autophagy related proteins LC3,Beclin1,p62,AMPK,mTOR and their phosphorylation levels were detected by using Western blot assay.Results Compared with the normal group,LVESD and LVED increased while FS and EF decreased in the DCM model group.Compared with the model group,the rats in the KXYXF and captopril group had decreased LVESD and LVED,and increased FS and EF.The expression of LC3-II/LC3-I and Beclin1 protein in the model group was lower than that in the normal group,and p62 protein expression was higher.The expression of LC3-II/LC3-I and Beclin1 protein in the KXYXF group and captopril group was higher than those in the model group,and p62 protein expression was lower.Compared with the normal group,the p-AMPK/AMPK ratio of myocardial tissue in the model group was reduced,and the p-mTOR/mTOR ratio was increased;compared with the model group,the p-AMPK/AMPK ratio of myocardial tissue in the KXYXF group and the captopril group was increased,and the p-mTOR/mTOR ratio was reduced.Conclusion KXYXF could promote autophagy of cardiomyocytes and improve cardiac function in DCM rats,and its mechanism may be related to AMPK/mTOR pathway.

Key words: dilated cardiomyopathy, Kangxian Yixin Formula, autophagy, rats

CLC Number: 

  • R285.5