主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2021, Vol. 44 ›› Issue (3): 252-258.doi: 10.3969/j.issn.1006-2157.2021.03.010

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Influence of envoy herbs on the effect mechanism of Xuefu Zhuyu Tang in treating NAFLD*

Cai Piao1, Song Li1, Liu Jiaxin1, Tang Songqi2, Huang Wei1#   

  1. 1 School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Sichuan 611137, China;
    2 School of Traditional Chinese Medicine, Hainan Medical University, Hainan 571199, China
  • Received:2020-10-23 Online:2021-03-30 Published:2021-03-30
  • Contact: Associate Prof. Huang Wei, Ph.D., Master's Supervisor. Chengdu University of Traditional Chinese Medicine. No. 1166 Liutai Avenue, Wenjiang District, Chengdu 611137. E-mail: gracehw@126.com
  • Supported by:
    National Natural Science Foundation of China (No. 81673879) Ethical review: Ethics Committee of Affiliated Hospital of Chengdu University of Traditional Chinese Medicine (No.2016010221)

Abstract: Objective To explore the influence of envoy herbs on the effect mechanism of Xuefu Zhuyu Tang (Blood Stasis Expelling Decoction, XFZYT) in the treatment of NAFLD. Methods C57BL/6J ApoE-/- mice models of NAFLD were established by intraperitoneal injection of subclinical lipopolysaccharide (LPS) (4ng/kg) combined with high-fat diet. During the establishment of the models, XFZYT, XFZYT minus the envoy herbs Niuxi (Two-toothed Achyranthes Root, Radix Achyranthis Bidentatae) and Jiegeng (Platycodon Root, Radix Platycodonis) (XFZYT-NJ) , and XFZYT minus Niuxi (XFZYT-N), were given by gavage to different groups of mice respectively for 28 days. Flow cytometry was used to detect the number of CD14+CD11b+Ly6G+ cells in whole blood. Immunofluorescence (IF) was used to observe neutrophil infiltration. Enzyme-linked immunosorbent assay (ELISA) was used to detect plasma keratin C-X-C motif chemokine ligand 1 (CXCL1) and macrophage inflammatory protein-2 also known as C-X-C motif chemokine ligand 2 (CXCL2), and myeloperoxidase (MPO) and CXCL2 content in liver tissue. Results Compared with the model group, the proportion of CD14+CD11b+Ly6G+ cells in the peripheral blood of mice in the XFZYT group was reduced (P<0.05). Such reduction was greater in the XFZYT group than in the XFZYT-NJ(P<0.05) group. XFZYT had the most significant effect of reducing neutrophil infiltration in liver tissue, compared with its variant formulas. While both XFZYT and its variant formulas reduced CXCL1 and CXCL2 in peripheral blood and MPO and CXCL2 in liver tissue (P<0.05), the former demonstrated greater reduction in CXCL2 in peripheral blood and MPO and CXCL2 in liver tissue than XFZYT-NJ(P<0.05). Conclusion XFZYT could prevent the formation of nonalcoholic fatty liver in C57BL/6J ApoE-/- mice. Its mechanism may be closely related to chemotaxis and activation of neutrophils in liver tissues. The envoy herbs could significant affect such mechanism.

Key words: Xuefu Zhuyu Tang, NAFLD, envoy herb, mouse

CLC Number: 

  • R285.5