主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2021, Vol. 44 ›› Issue (3): 280-288.doi: 10.3969/j.issn.1006-2157.2021.03.014

• Acupuncture & Moxibustion • Previous Articles    

Inflammatory regulation mechanism of moxibustion on rats with diminished ovarian reserve based on Nrf2/HO-1 signaling pathway*

Wang Qian, Lu Ge, Li Hongxiao, Shi Ling, Liang Shangjie, Shen Meihong#   

  1. School of Acupuncture, Moxibustion and Tuina, Nanjing University of Chinese Medicine, Jiangsu 210023, China
  • Received:2020-07-23 Online:2021-03-30 Published:2021-03-30
  • Contact: Prof. Shen Meihong, Ph.D., Doctoral Supervisor. School of Acupuncture, Moxibustion and Tuina, Nanjing University of Chinese Medicine. No. 138 Xianlin Avenue, Qixia District, Nanjing 210023. E-mail: mhshen@njucm.edu.cn
  • Supported by:
    National Natural Science Foundation of China (No. 81774408, No. 81973957), Research and Innovation Program for Graduate Students in Jiangsu Province (No. KYCX20_1541) Ethical review: Animal Ethics Committee of Nanjing University of Chinese Medicine (No. 201909A027)

Abstract: Objective To explore whether the protective effect of moxibustion on ovary of rats with diminished ovarian reserve (DOR) is related to the inflammatory regulation mediated by nuclear factor erythroid2-related factor 2 (Nrf2) /hemeoxygenase1 (HO-1) signaling pathway. Methods Fifty female rats were randomly divided into blank group, multi-glycosides of Tripterygium wilfordii (GTW) group, moxibustion group, all-trans retinoic acid (ATRA) group and dimethyl sulfoxide (DMSO) group, 10 in each group. The GTW group, moxibustion group, ATRA group and DMSO group were treated with Tripterygium wilfordii polyglycoside tablet suspension by gavage to establish DOR rat model. The blank group were given the same amount of normal saline by gavage daily. The moxibustion group was treated by moxibustion at bilateral BL23 on alternate days and CV4 and CV12 on the other days for 10 minutes each time one hour after intragastric administration. The ATRA group received intraperitoneal injection of ATRA suspension 30 minutes after intragastric administration and moxibustion intervention together with the moxibustion group 30 minutes later. The DMSO group received intraperitoneal injection of the same volume of DMSO solution 30 minutes after intragastric administration and the same moxibustion treatment as the ATRA group. The weight and the estrous cycle of rats were observed every morning. After the intervention, the coefficients of ovarian organs of the rats were calculated, the histological structure of ovaries was observed with HE staining, the changes in serum sex hormones and inflammatory factors were detected by ELISA, the expression levels of Nrf2, HO-1 protein and mRNA were detected by immunohistochemistry and PCR respectively. Results Compared with the blank group, the ovarian coefficients of rats in the GTW group decreased significantly (P<0.05), the detection rate of estrous cycle disorder increased significantly (P<0.01), the serum levels of FSH, T, INF-γ increased (all P<0.01), the levels of AMH(P<0.01, P<0.05) and IL-4(all P<0.01) decreased, and the expression levels of Nrf2 and HO-1 protein (all P<0.01) and mRNA (all P<0.05) in ovarian tissues decreased significantly. Compared with the GTW group, the ovarian coefficients of rats in the moxibustion group and the DMSO group increased (all P<0.05), the detection rates of estrous cycle disorder decreased (all P<0.01), the serum levels of FSH, T, INF-γ decreased (all P<0.01), the levels of AMH (P<0.01,p<0.05)and IL-4 increased (all P<0.01), the expression levels of Nrf2 and HO-1 protein and mRNA in ovarian tissues increased significantly (all P<0.01). Compared with the moxibustion group, the ovarian coefficients of rats in the ATRA group decreased (P<0.01), the detection rate of estrus cycle disorder increased (P<0.01), the serum levels of FSH, T, INF-γ increased (all P<0.01), the levels of AMH and IL-4 decreased (P<0.05, P<0.01), the expression levels of Nrf2 and HO-1 protein (all P<0.01) and mRNA (all P<0.05) in ovarian tissues decreased. Conclusion Moxibustion can attenuate the inflammatory injury induced by GTW in DOR rats and protect the ovary by activating Nrf2 / HO-1 signaling pathway.

Key words: moxibustion, diminished ovarian reserve, inflammatory regulation, Nrf2/HO-1 signaling pathway, rats

CLC Number: 

  • R246.3