主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2021, Vol. 44 ›› Issue (6): 500-509.doi: 10.3969/j.issn.1006-2157.2021.06.004

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Effects of Buyang Huanwu Tang on promoting the microstructural remodeling of brain tissue in rats with cerebral ischemia*

Feng Xuefeng, Li Manzhong, Zhan Yu, Yang Le, Lu Yun, Li Mingcong, Zhao Hui#   

  1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • Received:2021-02-25 Online:2021-06-30 Published:2021-06-25
  • Contact: Prof.Zhao Hui,Ph.D.,Doctoral Supervisor.School of Traditional Chinese Medicine,Capital Medical University. No.10 Xitoutiao, Youanmenwai, Fengtai District, Beijing 100069. E-mail: zhaohui8957@sina.com
  • Supported by:
    Beijing Natural Science Foundation (No. 7212161)

Abstract: Objective To observe the pharmacological effects and mechanism of Buyang Huanwu Tang (Yang-Supplementing and Five-Returning Decoction, BYHWT) on improving cerebral perfusion, protecting nerve cells and promoting axon growth and microstructure remodeling in rats with cerebral ischemia. Methods Cerebral ischemia model was induced by right middle cerebral artery occlusion. Male SD rats were randomly divided into sham group, model group and BYHWT group. The BYHWT group received 16.1 g/kg of BYHWT by gavage while the other two groups received the same amount of normal saline for 15 consecutive days, after which tissues were taken and examined. T2-weighted imaging (T2WI), arterial spin labeling (ASL) and diffusion tensor imaging (DTI) were applied to measure the infarct volume, analyze the changes in cerebral perfusion and detect the ultrastructure of the damaged brain region in cerebral ischemia rats respectively. Haematoxylin-eosin(HE) staining was used to observe the pathological damage of the injured brain region, immunofluorescence (IF) staining to detect expression levels of amyloid precursor protein (APP), NogoA and Nogo receptor (NgR), and real-time PCR (RT-RCR) to detect gene levels of NogoA and NgR and growth associated protein-43 (GAP-43) in order to evaluate axon growth and injury in rats with cerebral ischemia. Results MRI results revealed the BYHWT group had decreased infarct volume, increased cerebral perfusion of the infarcted core and peripheral cortex, and improved ultrastructure of periinfarct cortex, compared with the model group (P<0.05). HE staining showed the number of normal cortical neurons was increased while the number of abnormal neurons was decreased (P<0.01 or P<0.05), the degree of edema was reduced, and the density of nerve fibers was increased in the BYHWT group. According to IF staining results, compared with the model group, BYHWT group showed reduced positive expression of APP and NogoA in infarcted peripheral cortex and internal capsule (P<0.05 or P<0.01), and decreased positive expression of NgR in internal capsule(P<0.05). RT-PCR results indicated that BYHWT reduced gene expression levels of NogoA and NgR in peripheral tissue of infarct in the cerebral ischemia rats (P<0.05 or P<0.01). Conclusion BYHWT can effectively reduce the infarct volume, improve cerebral perfusion and brain ultrastructure, reduce nerve cell injury, promote axon growth and microstructural remodeling in rats with cerebral ischemia.

Key words: Buyang Huanwu Tang, cerebral ischemia, cerebral perfusion, nerve cells, axon growth, rats

CLC Number: 

  • R285.5