主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2021, Vol. 44 ›› Issue (6): 519-526.doi: 10.3969/j.issn.1006-2157.2021.06.006

• Science & Technology Theme • Previous Articles     Next Articles

Study on the expression levels of Ghrelin/GHSR-1a/GABA pathway key factors in models of gastroesophageal reflux disease with liver constraint pattern*

Chen Dongxue1, Qian Zhanhong1, Xia Congyuan2, Ma Ru3, Xu Yuan2, Zhang Liying4#   

  1. 1 School of Traditional Chinese Medicine, Inner Mongolia Medical University, Inner Mongolian 010110, China;
    2 Department of TCM Rheumatology & Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China;
    3 Beijing University of Chinese Medicine, Beijing 100029, China;
    4 Department of Spleen-stomach Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
  • Received:2021-01-03 Online:2021-06-30 Published:2021-06-25
  • Contact: Zhang Liying, Ph.D., Attending Physician. Xiyuan Hospital, China Academy of Chinese Medical Sciences. No.1 Xiyuangcaochang Road, Haidian District, Beijing 100091. E-Mail:zhangliying2000@163.com
  • Supported by:
    National Natural Science Foundation for Young Scientists of China (No. 82004355)

Abstract: Objective To investigate the expression levels of Ghrelin/GHSR-1a/GABA pathway key factors in rat model of gastroesophageal reflux disease (GERD) with liver constraint pattern. Methods 55 SD rats were randomly divided into the normal group, sham operation group, GERD group, liver constraint pattern group, and GERD with liver constraint pattern group, with 11 rats in each group. The GERD models were established by esophagogastroduodenal anastomosis (EGDA), the liver constraint pattern models were established by chronic unpredictable stimulation (CUS), and the GERD with liver constraint pattern models were established by EGDA combined with CUS. Sucrose preference test, open field test, and HE pathological observation of esophageal epithelium were performed. Ghrelin levels in serum and gastric tissue, GABA and GHSR-1a expressions in the hypothalamus was detected. Results Compared with the normal group and the GERD group respectively, the sucrose preference percentage and the total number of grids crossed (P<0.01) and the time spent in the central zone (P<0.01) by the GERD with liver constraint pattern group in the open field test were reduced, and the content of Ghrelin in the serum (P<0.01), the expressions of Ghrelin positive cells in the gastric tissue, and the expressions of GHSR-1a protein in the hypothalamus (P<0.01) were increased. Compared with the sham operation group, the contents of GABA in the hypothalamus of rats in the GERD with liver constraint pattern group were reduced (P<0.05). Conclusion EGDA combined with CUS can successfully establish the GERD with liver constraint pattern models. The expressions of the Ghrelin in the serum and gastric tissue and the GHSR-1a protein in the hypothalamus in the rat models may increase while the contents of GABA may decrease.

Key words: gastroesophageal reflux disease, liver constraint pattern, Ghrelin, GHSR-1a, GABA, model of combination of disease and pattern, rats

CLC Number: 

  • R228