ISSN 1006-2157　CN 11-3574/R

### Comparison of two chromatographic methods for the determination of sugars in Danshen Injection (lyophilized powder)*

LIU Jing1, HAN Chun-xia1, JIANG Yan1, WANG Gang-li1, LIN Rui-chao2#, DAI Zhong1, MA Shuang-cheng1

1. 1 Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 100050;
2 School of Chinese Materia Medica, Beijing University of Chinese Medicine
• Received:2014-12-04 Online:2015-08-30 Published:2015-08-30

Abstract: Objective To compare two chromatographic Methods and then to select an accurate, convenient and rapid method for the determination of sugars in Danshen Injection (lyophilized powder). Methods Firstly, HPLC-ELSD method was applied on an Alltech PrevailTM Carbohydrate ES (4.6 mm × 250 mm, 5 μm) column, and acetonitrile-water (76∶24) was used as the mobile phase on an isocratic elution with ELSD as the detector. Apart from that, IC method was adopted on a CarboPac PA20 (150 mm × 3 mm, S/N 003654 ) anion exchange column with the CarboPac PA20 ( 50 × 3 mm, S/N 003405) column as the guard column, and sodium hydrate solution (7 mmol/L) was used as eluent with the pulsed amperometric detector. To sum up, HPLC-ELSD and IC Methods described above were applied to determine the sugars in Danshen Injection (lyophilized powder) respectively. Results Under the chromatographic conditions mentioned above, HPLC-ELSD method could simultaneously determine the contents of fructose and glucose. Linear relationship, precision, repeatability and recovery rate of the method were all qualified; IC method could simultaneously determine arabinose, galactose, glucose, sucrose, mannose as well as fructose, and the method proved to be accurate and reliable. The determination Results of fructose and glucose by both Methods were basically the same. Conclusion IC method seems to be a better choice to determine the monosaccharides and disaccharide in Danshen Injection (lyophilized powder).

CLC Number:

• R284.1