主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (8): 551-555.doi: 10.3969/j.issn.1006-2157.2015.08.010

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Determination of three diterpenoids in Isodon excisoides using solid phase extraction-high performance liquid chromatography (SPE-HPLC)*

DAI Li-ping1,2,4, CHEN Sui-qing1,4, ZHAO Meng1, WANG Xiao-Xue1 , LU Xin-da1, WANG Zhi-min2,3#   

  1. 1 Henan College of Traditional Chinese Medicine, Henan 450046;
    2 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;
    3 National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine;
    4 Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan College of Traditional Chinese Medicine
  • Received:2015-03-16 Online:2015-08-30 Published:2015-08-30

Abstract: Objective To establish a method to simultaneously determine three diterpenoids in Isodon excisoides. Methods The procedure of SPE-HPLC was performed on a Venusil C18 column(4.6 mm×250 mm,5 μm) . The mobile phases was water and acetonitrile in gradient mode (0~12 min, 32%A; 12~18 min, 32~45%A; 18~35 min, 45%A). The flow rate was 1.0 mL·min-1. The column temperature was 30℃. The UV detection wave length was 230 nm. Results All the standard compounds showed a good linearity in the range of 0.056~0.448 g/L(r=0.999 2), 0.002 6~0.020 8 g/L(r=0.999 4), 0.016~0.128 g/L(r=0.999 7) for Kamebacetal-A, Kamebacetal-B, and Reniformin C, respectively. The average recoveries of the three diterpenoids were 97.53%, 96.02%, 98.23% with RSD 2.16%, 2.01%, and 2.10%, respectively. Conclusion This method is simple, accurate and effective. It could be used for quality control of ingredients in Isodon excisoides. It could also provide reference for the study of antitumor activity spectrum.

Key words: Isodon excisoides, diterpenoids, HPLC, content determination

CLC Number: 

  • R284.1