主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2020, Vol. 43 ›› Issue (8): 645-652.doi: 10.3969/j.issn.1006-2157.2020.08.006

• Chinese Medicinal Pharmacology • Previous Articles     Next Articles

Protective effect and mechanism of chebulagic acid on H9C2 cells injury induced by aconitine*

Han Shu, Liu Shuai, Zhang Qiao, Zhi Meiru, Liu Kaiyang, Tang Ya’nan, Han Xitao, Liu Ziqin, Li Fei, Du Hong#   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
  • Received:2020-02-03 Online:2020-08-30 Published:2020-08-27
  • Contact: Prof. Du Hong, Ph.D., Doctoral Supervisor. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Northeast Corner of the Intersection of Yangguang South Street and Baiyang East Road, Fangshan District, Beijing 102488,China. E-mail: duhong@vip.163.com
  • Supported by:
    National Natural Science Foundation of China (No. 81774004), Natural Science Foundation of Beijing (No.7182093)

Abstract: Objective To investigate the toxicity of aconitine to H9C2 cells by HCA, and to further explore the effect of chebulagic acid, the active component of He Zi (medicine terminalia fruit, Fructus Chebulae), on the cardiotoxicity induced by aconitine, and to explore its mechanism in reducing the toxicity of He Zi (medicine terminalia fruit, Fructus Chebulae). Methods Different concentrations of aconitine, chebulagic acid and aconitine + chebulagic acid were used to act on H9C2 cells respectively. Hoechst 33258 and other fluorescent probes were used to dye the cells. HCA technology was used to detect and analyze the effects of drugs of different concentrations on the number of H9C2 cells, the area of nucleus, the DNA content of nucleus, the fluorescence intensity of Ca2+ and the influence on function of mitochondria. Results 10,20 μmol/L aconitine caused Ca2 + overload in cardiomyocytes; 20 μmol/L aconitine caused mitochondrial dysfunction of cardiomyocytes in varying degrees after acting on cardiomyocytes for 24 hours; 5,10,20 μmol/L chebulagic acid had no obvious toxicity when acting on H9C2 cells alone; 10,20 μmol/L of chebulagic acid combined with 20 μmol/L aconitine restored the mitochondrial function and significantly reduced the fluorescence intensity of Ca2+, compared with using aconitine alone. Compared with the control group, there was no significant difference between the two groups. Conclusions Aconitine causes calcium overload and mitochondrial dysfunction in cardiomyocytes, which may be the main cause of cardiotoxicity induced by aconitine. Chebulagic acid in He Zi (medicine terminalia fruit, Fructus Chebulae) seems to inhibit the Ca2 + overload induced by aconitine, improve the mitochondrial function, and detoxify aconitine.

Key words: Cao Wu (kusnezoff monkshood root, Radix Aconiti Kusnezoffii) prepared with He Zi (medicine terminalia fruit, Fructus Chebulae), aconitine, chebulagic acid, H9C2 cell, high content analysis (HCA) technology, processing for detoxification

CLC Number: 

  • R285.5