主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE ›› 2021, Vol. 44 ›› Issue (3): 245-251.doi: 10.3969/j.issn.1006-2157.2021.03.009

• Science & Technology Theme • Previous Articles     Next Articles

Establishment and evaluation of disease-pattern rat model with IBS-D and spleen-kidney yang deficiency*

Su Xiaolan1, Liu Qian1, Han Boyu2, Zhang Tao1, Mao Xinyong1, Guo Song1, Du Sijing1, Yang Yang1, Wang Xin1, Wei Wei1#   

  1. 1 Department of Spleen and Stomach Diseases, Wang Jing Hospital of China Academy of Chinese Medical Sciences; Beijing Key Laboratory of TCM Diagnosis and Treatment of Functional Gastrointestinal Disorders, Beijing 100102, China;
    2 Department of Chinese Medicine, Beijing Longfu Hospital, Beijing 100010, China
  • Received:2020-11-15 Online:2021-03-30 Published:2021-03-30
  • Contact: Prof. Wei Wei, M.D., Chief Physician, Doctoral Supervisor. Wang Jing Hospital of China Academy of Chinese Medical Sciences. No. 6 Zhonghuan Nan Lu, Chaoyang District, Beijing 100102. E-mail:sxxtyy@sina.com
  • Supported by:
    National Natural Science Foundation of China (No.81774066,No.81973638,No.81820108033), National Pilot Project of Cooperation between Chinese and Western Medicine for Major and Refractory Diseases Ethical review: Animal Ethics Committee of Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences(No.02019-11-14-1)

Abstract: Objective To establish and evaluate a rat model with both a Western medicine disease, i.e. diarrhea-predominant irritable bowel syndrome (IBS-D) and a Chinese medicine pattern, i.e. spleen-kidney yang deficiency. Methods Eighteen suckling SD rats were randomly selected from 72 male suckling SD rats as the normal group, and the remaining 54 rats were separated from their mothers for 21 days to construct the IBS-D disease model. After the mother-child separation, the 54 rats were fed regularly for 1 week in adaptation. Then, they were randomly divided into high-dose, mid-dose and low- dose Fanxieye (Senna Leaf, Folium Senae) groups, 6 rats in each group. Each group were further divided into three groups and were given Fanxieye by gavage for 2,4 and 6 weeks respectively to establish the rat model with IBS-D and spleen-kidney yang deficiency. The macroscopic signs, visceral hypersensitivity, biological parameters and histopathological changes of rats in different groups were collected and analyzed to evaluate the model at the 2nd, 4th and 6th week since intragastric administration of Fanxieye. Results After 6 weeks of gavage with Fanxieye, compared with the normal group, rats in the high-dose Fanxieye group ate less, drank more, and had lower anal temperature, reduced grip strength, shorter weight-bearing swimming time, increased number of pellet-like feces, increased visceral sensitivity, and reduced levels of serum D-xylose, adrenocorticotropic hormone (ACTH) and serum cortisone (CORT) , all with statistical significance(P<0.05). There were no obvious histopathological changes in intestinal mucosa in each group. Conclusion The rat model with IBS-D and spleen-kidney yang deficiency can be constructed by mother-child separation superimposed with Fanxieye by gavage. Specifically, mother-child separation combined with high-dose Fanxieye by gavage for 6 weeks is an ideal method to establish such model.

Key words: diarrhea-predominant irritable bowel syndrome / IBS-D, spleen-kidney yang deficiency, rat model, combination of disease and pattern

CLC Number: 

  • R285.5