Correlation between structural shifts of gut microbiota and hyperuricemia in quails
JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE
2015, 38 (7):
Objective To explore the effect and potential mechanism of gut microbiota on the development of hyperuricemia(HUA).
Methods Altogether 48 Difake male quails were randomly diveded into four groups (
n=12 each) according to body weight: normal group(N group), model group(M group), antibiotics and normal diet group(Ab+N group), and antibiotics and high-purine diet group(Ab+M group). Quails in N group and Ab+N group were fed with normal diet, and those of other two groups were fed with high-purine diet; at the same time, quails in N group and M groups drank clean tap water, and those of Ab+N group and Ab+M group drank water containing ampicillin (150 mg/L) and neomycin (75 mg/L) for consecutive 28 days. The microbiota structure of quail gut was determined by PCR-DGGE, while the concentration of endotoxin (LPS) in plasma and cecal content, serum uric acid (UA), xanthine oxidase (XO) activity in serum and liver were measured by automatic biochemical analyzer or multiscan spectrum.
Results Compared with N group, the gut microbiota structure of M group changed, as well as the concentration of LPS in plasma and cecal contents, the activity of XO in serum and liver, and serum UA all increased significantly (
P<0.01); however, the indicators mentioned above showed no significant differences in Ab+N group and Ab+M group (
P>0.05). Compared with M group, the concentration of LPS in plasma and cecal contents, the activity of XO in liver, and serum UA in Ab+N group and Ab+M group all decreased dramatically(
Conclusion High-purine diet induced structural shifts of gut microbiota and HUA in quails. The concentration of LPS, metabolite of gut microbial flora, is positively related to the activity of XO and serum concentration of UA. And the occurrence of hyperuricemia maybe is attributed to activation effect of LPS on XO activity.
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