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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 January 2014, Volume 37 Issue 1 Previous Issue    Next Issue
    On “all diseases derived from qi”
    FENG Xing-zhong, WANG Yong-yan
    2014, 37 (1):  5-8.  doi: 10.3969/j.issn.1006-2157.2014.01.001
    Abstract ( 455 )   PDF (809KB) ( 285 )   Save
    “All diseases derived from qi” comes out of Suwen·Jutonglunpian. Qi is the basic substance of the human body, and the movement of qi is the source power of human life, so dysfunction of qi will lead to many diseases. It is very important to regulate qi to cure diseases. The concept of “all disease derived from qi” reflects TCM dual attributes of natural science and the humanities. For example, diabetes, as a kind of psychosomatic disease, is characterized by disorders of qi movement, and regulation of qi movement is the key in treatment.
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    Discrimination of moderate pulse and combined pulse in Shanghanlun and Jinguiyaolue
    CHEN Li-ping, WANG Tian-fang, XUE Xiao-lin, ZHAO Fu-cheng, GE Jian, YU Xiao-fei
    2014, 37 (1):  9-11.  doi: 10.3969/j.issn.1006-2157.2014.01.002
    Abstract ( 547 )   PDF (449KB) ( 280 )   Save
    It is revealed that moderate pulse was given by various meanings and associated with various diseases in Shanghanlun and Jinguiyaolue as the differentiation and analysis about moderate pulse, and its associated characteristics and clinical significance were accomplished. Its conceptions and logical definitions were poorly distinguished. Consequently, conceptions of moderate pulse should be clarified. The pulse, described as neither fast nor slow, with a neutral characteristic should be renamed as even pulse. The one with a slow characteristic should be renamed as slow pulse. The one with a flaccid characteristic should be renamed as flaccid pulse.
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    Influences of Qizhi Yifei Capsule medicated serum on expressions of Smad3 mRNA, Smad4 mRNA and Smad7 mRNA in lung fibroblasts stimulated by TGF-β1
    FENG Cui-ling, LIU Zhi-kun, HOU Ya-jing, GAO Yong-hong, SUN Yi-kun
    2014, 37 (1):  18-21.  doi: 10.3969/j.issn.1006-2157.2014.01.005
    Abstract ( 481 )   PDF (579KB) ( 283 )   Save
    Objective To definite the regulation effect of Qizhi Yifei Capsule medicated serum on signal transduction pathway of TGF-β1/Smads of fibroblasts in normal rats. Methods The fibroblasts were separated from lung tissue in rats after birth for 2-3 days, and then randomly divided into blank group, model group and medicated serum group. Firstly model group and medicated serum group were given DMEM (containing 2.5 ug/L TGF-β1) and blank group was given fresh non-serum DMEM, and secondly blank group and model group were given DMEM with blank serum (5%) and medicated serum group was given DMEM with Qizhi Yifei Capsule medicated serum (5%). The expressions of Smad3 mRNA, Smad4 mRNA and Smad7 mRNA were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR) after 48 h and 72 h. Results In model group, the expressions of Smad3 mRNA and Smad4 mRNA increased after 48 h, and those of Smad3 mRNA, Smad4 mRNA and Smad7 mRNA increased after 72 h compared with blank group (P<0.05). In medicated serum group, the expressions of Smad3 mRNA and Smad4 mRNA decreased and expression of Smad7 mRNA increased after 48 h, and the expressions of Smad3 mRNA and Smad4 mRNA decreased after 72 h compared with model group (P<0.05). Conclusion Qizhi Yifei Capsule can inhibit the proliferation of lung fibroblasts stimulated by TGF-β1 through regulating the conduction of signal transduction pathway of TGF-β1/Smads.
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    Protective effect of main component combinations of Fuzi (Radix Aconiti Lateralis praeparata) and Gancao (Radix Glycyrrhizae) on passage myocardial cells injured by aconitine in rats
    ZHOU Tian-mei, YANG Jie-hong, WAN Hai-tong, ZHANG Yu-yan, ZHOU Hui-fen
    2014, 37 (1):  22-26.  doi: 10.3969/j.issn.1006-2157.2014.01.006
    Abstract ( 441 )   PDF (611KB) ( 240 )   Save
    Objective To investigate the protective effects and mechanism of the main component combinations of Fuzi (Radix Aconiti Lateralis praeparata) and Gancao (Radix Glycyrrhizae) on passage myocardial cells injured by aconitine in rats. Methods The influences of main component combinations of Fuzi and Gancao on survival rate of myocardial cells were detected by using methyl thiazolyl tetrazolium test (MTT), and activities of succinodehydrogenase (SDH), Na+-K+ ATPase and Ca2+-Mg2+ ATPase were determined by using kits of SDH, Na+-K+ ATPase and Ca2+-Mg2+ ATPase. Results In aconitine group (120 umol/L), survival rate of myocardial cells and activities of SDH, Na+-K+ ATPase and Ca2+-Mg2+ ATPase decreased significantly. In all combination groups, survival rate of myocardial cells and activities of SDH, Na+-K+ ATPase and Ca2+-Mg2+ ATPase were recovered in varying degrees (P<0.05 or P<0.01). The recovery of survival rate of myocardial cells was the most significant in combination group 3 (P<0.01), recovery of SDH activity was the most significant in combination group 5 (P<0.01), and recoveries of Na+-K+ ATPase and Ca2+-Mg2+ ATPase were the most significant in combination group 4 (P<0.01) compared with verapamil group (P<0.01 or P<0.05). Conclusion The main component combinations of Fuzi and Gancao can protect myocardial cells from injury and play decreasing toxicity role through regulating the energy metabolism of SDH, Na+-K+ ATPase and Ca2+-Mg2+ ATPase.
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    Evolvement rule and biological basis of TCM syndromes in rat depression model induced by chronic stress or reserpine antagonisim
    LI Yu-bo, SUN Yu-xiu, MA Xue-ling, XUE Xiao-xing, CHAI Xin-lou, CHEN Jian-xin, ZHAO Hui-hui, GUO Shu-zhen, WANG Wei-ming, WANG Wei
    2014, 37 (1):  27-32.  doi: 10.3969/j.issn.1006-2157.2014.01.007
    Abstract ( 415 )   PDF (721KB) ( 277 )   Save
    Objective To compare rat depression model induced by chronic unpredictable mild stress (CUMS) or reserpine antagonisim, and describe the evolvement rule and initially explain molecular mechanism from the angle of TCM syndromes. Methods The rat depression model was established by applying CUMS or intraperitoneal injection of reserpine. The syndromes were distinguished in rats through ethological determination and equivalent transforming from clinical symptoms to macroscopic surface features. The content of serum noradrenalin (NE) was detected by using enzyme-linked immunosorbent assay (ELISA) and content of 5-hydroxytryptamine (5-HT) in cerebral hippocampal CA2 zone was detected by using immunohistochemistry technique. Results The evolvement rule of syndromes showed as follows: CUMS group had syndrome of liver-qi depression 2 weeks after stress and syndrome of liver depression and spleen deficiency 6 weeks after stress, and reserpine group had syndrome of spleen-qi deficiency 2 weeks after injecting and syndrome of liver depression and spleen deficiency 4 weeks after injecting. The level of serum NE increased in CUMS group (P<0.05) after 4 weeks and decreased (P<0.05) after 8 weeks, and continuously decreased (P<0.05) in reserpine group. The level of brain 5-HT was significantly lower in CUMS group and reserpine group than that in normal group (P<0.05), but there was no significant difference between CUMS group and reserpine group (P>0.05). Conclusion The syndromes of depression model induced by CUMS develop gradually from syndrome of liver-qi depression to syndrome of liver depression and spleen deficiency, and those of depression model induced by reserpine antagonisim, from syndrome of spleen-qi deficiency to syndrome of liver depression and spleen deficiency. The difference in the evolvement of syndromes is correlated to the levels of 5-HT and NE.
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    Influences of antagonism combinations of Gancao (Radix Glycyrrhizae) and Gansui (Radix Euphordiae Kansui) in modified Gansui Banxia Tang on hepatic and renal functions in rats with ascites
    LIU Hai-yan, ZHONG Gan-sheng, LIU Jia, WANG Xi, LI Yi-wen, LIU Yun-xiang, GAO Yuan, OU Li-na
    2014, 37 (1):  33-38.  doi: 10.3969/j.issn.1006-2157.2014.01.008
    Abstract ( 421 )   PDF (692KB) ( 243 )   Save
    Objective To discuss whether the efficacy or tocicity being correlated to the antagonism combinations of Gancao (Radix Glycyrrhizae) and Gansui (Radix Euphordiae Kansui) in modified Gansui Banxia Tang through observing the influences of the antagonism combinations on hepatic and renal functions in rats with malignant ascites. Methods Wistar rats were divided, according to weight, into blank group, model group, furosemide group, whole formula group of Gansui Banxia Tang (whole formula group), non-Gansui group, non-Gancao group and non-Gansui non-Gancao group. Besides of normal feeding, furosemide group, whole formula group, non-Gansui group, non-Gancao group and non-Gansui non-Gancao group were intragastrically given corresponding medicinal (10 mL/kg), and blank group and model group were orally given distilled water (10 mL/kg). After 9 d, all rats were fasted except of water for 12 h. The blood samples were collected and centrifugated for detecting relative indexes of hepatic and renal functions. Results Compared with model group, AST/ALT decreased (P<0.01), ALP increased (P<0.05), total protein (TP) increased (P<0.05), ratio of albumin (ALB) to A/G increased (P<0.05 or P<0.01), blood urea nitrogen (BUN) decreased (P<0.05) and creatinine (CRE) decreased (P<0.05) in whole formula group. Compared with whole formula group, AST/ALT increased (P<0.05 or P<0.01) and ratio of ALB to A/G decreased (P<0.05 or P<0.01) in non-Gansui group, non-Gancao group and non-Gansui non-Gancao group. Conclusion In whole formula group and the groups without one or two antagonism medicinal, there is no obvious toxicity observed for hepatic and renal functions, while the medicinal efficacy is higher in whole formula group. Antagonism combinations may have effect of improving medicinal efficacy.
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    Influence of Puerarin Injection on expression of GRP78 mRNA in HK-2 cells induced by high glucose
    NIE Yan-na, LI Shu-yu, CUI Lei, YI Yue-e, CHAI Xin-lou, WU Ying, WANG Yu-nong, JIA De-xian, WANG Qian
    2014, 37 (1):  39-42.  doi: 10.3969/j.issn.1006-2157.2014.01.009
    Abstract ( 420 )   PDF (545KB) ( 273 )   Save
    Objective To study the influences of Puerarin Injection on the proliferation of HK-2 cells and the expression of glucose regulated protein 78KD (GRP78) induced by high glucose. Methods The concentration gradient of puerarin was set as 5 g/L, 10 g/L, 20 g/L, 40 g/L, 80 g/L, 160 g/L, 320 g/L and 640 g/L. The proliferation of HK-2 cells was detected by using methyl thiazolyl tetrazolium test (MTT). After passage and serum-free adhere-wall culturing for 24 h, HK-2 cells were divided into normal group, model group and treatment group (with Puerarin Injection) and were cultured continuously for 48 h. The total RNA were extracted from all groups and expression of GRP78 mRNA was detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results The inhibitory effect of Puerarin Injection on the proliferation of HK-2 cells was reinforced as the increase of Puerarin Injection’s dose. After 48 h, the expression of GRP78 mRNA increased in model group compared with normal group (P<0.01), and the expression increased in treatment group compared with model group (P<0.01). Conclusion Puerarin Injection has inhibitory effect on the proliferation of HK-2 cells. After high glucose stimulation for 48 h, ERS stress reaction will be induced, and Puerarin Injection can improve correct protein folding and recover intracellular homeostasis through increasing the expression of GRP78 mRNA.
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    Influence of emodin on contraction of in vitro ileum smooth muscle in rats
    GAO Liu, HU Pan, HAN Xiao, JIANG Li-li, YANG Jing, MA Hui-jie, QIAO Xiao-wen, CHANG Ai-rong, ZHOU Ming-zhong
    2014, 37 (1):  43-47.  doi: 10.3969/j.issn.1006-2157.2014.01.010
    Abstract ( 391 )   PDF (679KB) ( 235 )   Save
    Objective To observe the influence of emodin on contraction of in vitro ileum smooth muscle in rats, and investigate the mechanism. Methods The samples of in vitro ileum were randomly divided into control group, 1 μmol/L emodin group, 5 μmol/L emodin group, 10 μmol/L emodin group, 20 μmol/L emodin group, propranolol+emodin group (PRO+emodin group), glibenclamide+emodin group (GLI+emodin group), L-NAME+emodin group, calcium-free control group and calcium-free emodin group (each n=6). The rats were sacrificed by applying cervical dislocation for isolating the ileum. The samples of ileum segments were connected with tension transducer, which were then put into oxygen saturation K-H solution. The influences of emodin on contraction tension (TE), amplitude (AM) and frequency (FR) of ileum smooth muscle were recorded by using BL-420+ biological signal processing system. Results Emodin reduced significantly contraction AM of rat ileum smooth muscle (P<0.05 or P<0.01) showing a concentration dependence, and had no significant influences on contraction FR and TE. Propranolol (P<0.01) and glibenclamide (P<0.01) partially blocked up the inhibitory effect of emodin on ileum smooth muscle, but L-NAME had no effect. The contraction of ileum smooth muscle induced by calcium chloride could be inhibited by emodin (P<0.05). Conclusion Emodin can significantly attenuate the contraction AM of rat ileum smooth muscle and has no effects on contraction TE and FR, which may be related to activating β-adrenergic receptor, exciting ATP sensitive potassium channel and inhibiting extracellular calcium influx.
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    Influences of eye acupuncture on expressions of BDNF and p75NTR and its cerebral protective effect in rats with cerebral ischemia-reperfusion injury
    MA Xian-de, WANG Shou-yan, ZHANG Wei
    2014, 37 (1):  48-52.  doi: 10.3969/j.issn.1006-2157.2014.01.011
    Abstract ( 384 )   PDF (764KB) ( 378 )   Save
    Objective To observe the influences of eye acupuncture on expressions of brain-derived neurotrophic factor (BDNF) and p75 neurotrophic receptor (p75NTR) and its cerebral protective effect in rats with cerebral ischemia-reperfusion injury, and discuss the therapeutic mechanism of eye acupuncture. Methods SD rats (n=62) were randomly divided into blank group (n=11), sham-operation group (n=11)and model copy group (n=40). The model of cerebral ischemia-reperfusion was established by using suture method, and successfully modeled rats (n=32) were divided into model group and eye acupuncture group. The eye acupuncture group was given needling in the acupoints in areas of liver, upper energizer, lower energizer and kidney according to the acupoint selection methods in human body after reperfusion for 2 h, once every 8 h for 10 times. The rats were killed 30 min after the last needling for peeling ischemic half dark cerebral tissue, and the gene and protein expressions of BDNF and p75NTR were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry technique. Results Compared with blank group, the gene and protein expressions of BDNF and p75NTR increased significantly in model group (P<0.01 or P<0.05). Compared with model group, the gene and protein expressions of BDNF increased significantly (P<0.01), and gene and protein expressions of p75NTR decreased significantly (P<0.01 or P<0.05) in eye acupuncture group. Conclusion The expressions of BDNF and p75NTR will increase for antagonizing brain injury after cerebral ischemia-reperfusion. Eye acupuncture can protect brain through up-regulating the expression of BDNF and down-regulating the expression of p75NTR.
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    Influences of needling chorea-tremble controlled zone on expressions of dopaminergic neurons and BDNF in mice with Parkinson’s disease
    FENG Jing, SUN Hong-mei, WANG Yuan-yuan, XU Hong, WU Hai-xia, HE Xin, GAO Yu-shan, ZHANG Shu-jing, GONG Xiao-gang
    2014, 37 (1):  53-57.  doi: 10.3969/j.issn.1006-2157.2014.01.012
    Abstract ( 437 )   PDF (785KB) ( 324 )   Save
    Objective To investigate the protective effect and possible mechanism of acupuncture on dopaminergic neurons (DA neurons) through observing the influences of needling chorea-tremble controlled zone on expression of substantia nigra brain derived neurotrophic factor (BDNF) in mice with Parkinson’s disease (PD). Methods Male C57BL/6 mice were randomly divided into normal group, model group, needling group, madopar group and needling+madopar group. The later 4 groups were given intraperitoneal injection of MPTP for establishing PD model, and then the later 3 groups were given corresponding treatment for 28 d. The ethological changes were observed in mice by using climbing method. The pathological changes of midbrain substantia nigra dopaminergic neurons were observed after HE staining. The loss of DA neurons was detected by applying immunohistochemistry technique and expression of BDNF was detected by using Western blot method. Results The ethological scores increased significantly in needling group, madopar group and needling+madopar group compared with model group (P<0.05). The count of DA neurons was higher in model group than that in normal group (P<0.05), and was higher in needling+madopar group than that in madopar group (P<0.05). The expression of BDNF decreased significantly in model group compared with normal group (P<0.05), and increased significantly in needling+madopar group compared with madopar group (P<0.05). Conclusion Needling chorea-tremble controlled zone combining madopar can treat PD through increasing the expression of BDNF and decreasing the loss of DA neurons in mice with PD.
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    Active constituents of inhibiting EGFR in ehtyl acetate extracts from Jixueteng (Caulis Spatholobi) analyzed by using UPLC-Q-TOF-MS
    WANG Dong-mei, LIN Sen-sen, ZHENG Si-hao, HUANG Lin-fang
    2014, 37 (1):  58-62.  doi: 10.3969/j.issn.1006-2157.2014.01.013
    Abstract ( 426 )   PDF (851KB) ( 254 )   Save
    Objective To screen the extracts with the inhibitive activity on epidermal growth factor receptor (EGFR) from Jixueteng (Caulis Spatholobi) by using the technique of homogeneous time-resolved fluorescence (HTRF), and to analyze and identify these active constituents by applying ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Methods The crude material of Jixueteng was percolated with petroleum ether, and then extracted with ethanol and ethyl acetate and boiled in water to obtain 4 extract fractions. The inhibitive effect of ethyl acetate extract on ERFR was detected by using HTRF and inhibitive rate was calculated. The UPLC/Q-TOF-MS procedure was performed on the chromatographic column of ACQUITY UPLC BEH C18, and the mobile phase was 0.1% formic acid solution (A)-acetonitrile (B) scanned in 200-400 nm. The data was collected by using ESI ion source in a positive ion mode. Results The ethyl acetate fractions of Jixueteng showed a strong inhibitive activity on EGFR and IC50 was 2.277 μg/mL. There were 15 chemicals identified from these active fractions, and their main constituents were flavonoids, among them 10 were as follows: (epi) catechin- (epi) catechin, myricetin -3' -O-β-D- xyloside, (epi) afzelechin- (epi) catechin, ononin, formononetin, afrormosin, epigallocatechin, vomifoliol -9-O-β-D- xylopyranosyl (1→6)-β-D- glucopyranside, luteolin-7-O-glucoside and (epi) gallocatechin-(epi) catechin. There were other 5 constituents unknown. The major active constituents are (epi) catechin-(epi) catechin, epigallocatechin and formononetin. Conclusion The flavonoids in ethyl acetate extracts from Jixueteng has a strong inhibitive activity on EGFR. The study hopes to provide theoretic bases for the anticancer application of Jixueteng and further tracking the separation of its active constituents.
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    Syndrome factors in high risk group of stroke
    LIU Yue, ZHANG Yun-ling, LIU Jin-min,ZHOU Chun-yu,GUO Rong-juan,CHEN Zhi-gang,ZHANG Zhi-chen,GAO Fang,ZHANG Zhi-jun,YANG Xiu-quan,ZHENG Hong
    2014, 37 (1):  63-67.  doi: 10.3969/j.issn.1006-2157.2014.01.014
    Abstract ( 397 )   PDF (741KB) ( 258 )   Save
    Objective To discuss the syndrome factors in high stroke-risk group, and provide the basis of syndrome differentiation and treatment for preventing and curing stroke. Methods An observation table for high stroke-risk group was designed by using prospective method, and EpiData3.1 software was applied to establish database. The accuracy of data was ensured through double researchers and double entry. The patients with stroke (n=2536) were screened and given syndrome differentiation. The distribution characteristics of TCM symptoms and distribution laws of syndrome factors were discussed through frequency analysis and factor analysis. Results The factor analysis got 7 common factors (syndrome factors). The analysis on the distribution of syndrome factors in 2 536 cases showed that the syndrome factor of yin deficiency counted for 20.5%, phlegm, 17.7%, qi deficiency, blood stasis, 13.9%, yang deficiency, 13.5%, fire, 11.7% and qi stagnation, 14.8%, 7.9%, and synthetic analysis showed that excess syndromes counted for 51.2%. Conclusion The major syndrome factors in high stroke-risk group are yin deficiency, phlegm, qi stagnation and blood stasis, and secondary ones are yang deficiency, fire and qi stagnation. The syndrome factors related to disease locations are brain involving mainly kidney and liver, and secondly spleen, stomach and heart.
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    Syndrome factors of multiple sclerosis in 500 patients
    FAN Yong-ping, WU Wei
    2014, 37 (1):  68-70.  doi: 10.3969/j.issn.1006-2157.2014.01.015
    Abstract ( 491 )   PDF (763KB) ( 311 )   Save
    Objective To study on establishing quantized criteria for TCM syndromes of multiple sclerosis. Methods The patients (n=500) were chosen according to TCM four examinations. The combinations of TCM symptom entries and combinations of TCM syndrome factors were extracted by using entropy-based complex system partition method, and contribution degrees of all symptom entries to corresponding combinations of TCM syndrome factors were determined by using multiple regression method. The diagnostic thresholds were established with ROC curves of diagnostic test. Results There were 6 combinations of TCM syndrome factors got from entropy clustering results, and they were liver-kidney yin deficiency, spleen-kidney yang deficiency, spleen qi deficiency, blood stasis, phlegm dampness and phlegm heat and engendering wind. Different symptom entries had different contribution degrees to corresponding combinations of TCM syndrome factors, and all combinations of TCM syndrome factors had different diagnostic thresholds, which had higher sensitivity and specificity through the retrospective verification taken experts’syndrome differentiation as the gold standard. Conclusion The Syndrome Diagnostic Scale of Multiple Sclerosis established by applying various statistical methods has good diagnostic performance and conforms to clinical demands.
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