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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 June 2014, Volume 37 Issue 6 Previous Issue    Next Issue
    Influence of qi-monism ideology on theory in Neijing
    HE Juan
    2014, 37 (6):  365-368.  doi: 10.3969/j.issn.1006-2157.2014.06.001
    Abstract ( 578 )   Save
    In the theory of qi-monism, original qi is introduced to explain the generation and nature of the universe, which belongs to the category of ontology in ancient philosophy. The basic content of Chinese ancient natural philosophy includes theory of qi-monism, yin-yang theory, and five phase theory. However, the theory of qi-monism plays a significant role in the formation of ideology of traditional Chinese medicine in Neijing, such as holistic conception of unity of heaven and man, thinking mode of analogy, theory of qi transformation, life conception of taking five viscera as the core, and theory of kidney qi governing the innate foundation.
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    Establishment of disease-syndrome combination animal model of ischemia stroke-heat toxin syndrome
    WANG Feng-li, LIU Xue-mei, ZHANG Yun-ling, WANG Jian-wei, ZHENG Hong, ZHANG Xin-yang, WANG Xin-xiang, YAN Yan, TANG Xu
    2014, 37 (6):  377-381.  doi: 10.3969/j.issn.1006-2157.2014.06.005
    Abstract ( 477 )   Save
    Objective To explore the preparing method and reviewing system for establishing disease-syndrome combination rat model of ischemia stroke-heat toxin syndrome. Methods The rat model of ischemia stroke-heat toxin syndrome was established with intraperitoneal injection of carrageenan combining thread approach of middle cerebral artery occlusion (MCAO). The representations of rat tongue, auricle, nail and, tail, and changes of body temperature, relative coagulation indexes and cerebral morphology were observed and detected at different time points for establishing a review system. Results The model rats showed some stasis-heat representations including black tongue, thickened sublingual collateral vessels, red auricle, swelling and purple nail and dark tail after the intraperitoneal injection of carrageenan. After MCAO, the representations were more significant in model group than those in sham-operation group and exacerbated persistently. The body temperature increased sharply after 24 h, and was still higher in model group than that in sham-operation group after 72 h, which was similar to TCM heat toxin syndrome. The coagulation indexes showed that whole blood viscosity increased but blood was low coagulation state in model group at different time points. The changes of cerebral morphology were accorded with ischemia changes. Conclusion The intraperitoneal injection of carrageenan combining thread approach of MCAO can be used for establishing rat model of ischemia stroke-heat toxin syndrome. The review system of the model can be consisted of representations, body temperature, coagulation indexed and changes of cerebral morphology.
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    Influences of Danggui Buxue Tang on function of circulating endothelial progenitor cells and Bcl-2 expression
    QIN Zhen, HUANG Shui-qing, WEI Zheng-xin
    2014, 37 (6):  382-386.  doi: 10.3969/j.issn.1006-2157.2014.06.006
    Abstract ( 461 )   Save
    Objective To observe the influences of Danggui Buxue Tang on function of circulating endothelial progenitor cells (EPCs) and Bcl-2 expression. Methods Human mononuclear cells in peripheral blood were isolated and cultured for 7 d. After adherent cells identified, EPCs were randomly divided into 6 groups: blank group, control group, simvastatin group, and high-dose, mid-dose and low-dose Danggui Buxue Tang groups (high-dose group, mid-dose group and low-dose group). Blank group was cultured with nutrient solution without medicated serum, control group, 0.2% bovine serum albumin, simvastatin group, 0.1 μmol/L simvastatin, and high-dose group, mid-dose group and low-dose group, respectively, 4 g, 2 g and 1 g Danggui Buxue Tang. The supermatant was eliminated 24 h after culturing, and then all groups were given nutrient solution without medicated serum for continuous culturing for 24 h. The proliferation ability, adhesive force, migration ability and tubule formation of EPCs were detected, respectively, by using MTT, adhesion test, Transwell assay and in vitro angiogenesis kit. The expressions of Bcl-2 mRNA and Bcl-2 protein were detected respectively by using RT-PCR and Western blot. Results All functions of EPCs were significantly improved and expression of Bcl-2 mRNA was up-regulated in high-dose group and simvastatin group (P<0.05). The proliferation, migration of and tubule formation of EPCs were improved but adhesive force and expression of Bcl-2 mRNA were not affected in mid-dose group and low-dose group. The expression of Bcl-2 protein was up-regulated in high-dose group, mid-dose group, low-dose group and simvastatin group (P<0.05). Conclusion Danggui Buxue Tang can improve the function of EPCs through up-regulating the expressions of Bcl-2.
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    Mobilizing effect of panax notoginseng saponins on marrow mesenchymal stem cells in model of cerebral ischemia-reperfusion injury
    ZHANG Bao-xia, ZHANG Jin-sheng, DU Mei-mei, WANG Ping, ZHU Hui-fang, ZHANG Yang-yang
    2014, 37 (6):  387-392.  doi: 10.3969/j.issn.1006-2157.2014.06.007
    Abstract ( 433 )   Save
    Objective To investigate the mobilizing effect of panax notoginseng saponins (PNS) on marrow mesenchymal stem cells after cerebral infarction. Methods The rat model of MCAO was established by referencing Zea-Longa method. The modeled rats were randomly divided into model group and high-dose, mid-dose and low-dose Xuesaitong Capsules groups (high-dose group, mid-dose group and low-dose group), and then according to time points divided into 1-d, 7-d, 14-d and 28-d subgroups. High-dose group, mid-dose group and low-dose group were treated with Xuesaitong Capsules (60 g/L, 40 g/L and 20 g/L, 10 g/kg once a day), and normal group and model group were orally given normal saline water once a day for 28 d. The positive percentage of CD54, CD106, CD105 and CD117 in peripheral blood and bone marrow were detected by using flow cytometer at different time points, and expression of stem cell factor (SCF) in peripheral blood and bone marrow was detected by using ELISA at different time points. Results SCF level and positive percentage CD117 and CD105 increased in high-dose group, mid-dose group and model group on the first day, reached the peak on the 14th day, and then decreased gradually, but the increased trend is superior in high-dose group and mid-dose group to model group (P<0.05). The expressions of CD54 and CD106 reached the peak in model group, high-dose group, mid-dose group and model group on the first day and then decreased gradually, and decreased in mid-dose group and high-dose group at all time points compared with model group especially in high-dose group (P<0.05). Conclusion PNS can mobilize marrow mesonchymal stem cells from bone marrow to peripheral blood and promote number of homing stem cells through improve SCF level and decreasing expressions of CD54 and CD106.
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    Influence of therapy of reinforcing kidney and regulating qi on BDNF expression in rats with post-encephalorrhagia depression
    LUO Bin, TANG Qi-sheng, LI Xia, XU Xiang-qing
    2014, 37 (6):  393-396.  doi: 10.3969/j.issn.1006-2157.2014.06.008
    Abstract ( 420 )   Save
    Objective To observe the expression of brain-derived neurotrophic factor (BDNF) in rats with post-encephalorrhagia depression, and study the mechanism of therapy of reinforcing kidney and regulating qi (Yinao Jieyu Fang) in treatment of post-encephalorrhagia depression. Methods The rats (n=32) with the similar scores of open-field test were chosen and randomly divided into normal group, model group, Chinese medicial group (treated with Yinao Jieyu Fang) and Western medicial group (treated with Baiyoujie, each n=8). The changes of BDNF expression were observed by using immunohistochemistry technique in normal group, model group, Chinese medicial group and Western medicial group. Results In model group, the areal density (AD), optical density (OD) in hippocampus CA1 and CA3 zones and OD in CA2 zone of BDNF positive cells decreased significantly compared with normal group (P<0.05), and AD of BDNF positive cells in CA1 zone, and AD and OD in CA3 zone increased significantly in Western medicinal group compared with model group (P<0.05). Compared with normal group, AD and OD in cingulum cortex and AD in hypothalamus decreased significantly in model group (P<0.05). In Chinese medicial group, AD and OD of BDNF positive cells increased in CA3 zone compared with model group (P<0.05), OD increased significantly in cingulum cortex compared with model group, and AD increased in hypothalamus compared with model group (P<0.05). Conclusion Yinao Jieyu Fang can promote the repair and regeneration of neurons and relive depression symptoms through increasing BDNF expression in rats with post-encephalorrhagia depression.
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    Influences of Bushen Shengxue Jiedu Fang on expressions of erythropoiesis-related factors in mice with aplastic anemia
    DONG Bin, LIU Juan, LOU Li-xia, WU Ai-ming, LV Xi-ying, CHAI Li-min
    2014, 37 (6):  397-400.  doi: 10.3969/j.issn.1006-2157.2014.06.009
    Abstract ( 429 )   Save
    Objective To investigate the influence of Bushen Shengxue Jiedu Fang on erythropoiesis in myelosuppression mice induced by physical and chemical compound factors. Methods BALB/C mice(n=50) were randomly divided into blank group, model group, and low-dose, mid-dose and high-dose Chinese medicinal groups (low-dose group, mid-dose group and high-dose group). The model of aplastic anemia (AA) was established with 60Co-γ ray and cyclophosphamide injection. Bushen Shengxue Jiedu Fang was used for intervention in low-dose, mid-dose and high-dose groups (6.14 g/kg, 12.29 g/kg and 24.57 g/kg). The changes of erythrocyte parameters were detected by using blood cell counter. The level changes of erythrogenin (EPO) and interferon γ (IFNγ) were detected by using ELISA, and expressions of EPO receptor and IFNγ receptor were detected by using RT-PCR. Results Bushen Shengxue Jiedu Fang increased erythrocyte parameters, relieved anemia symptoms, increased the level of serum EPO and improved significantly the expressions of EPO receptor gene in bone marrow cells, decreased the content of IFNγ in peripheral blood, and inhibited the expression of IFNγ receptor gene. Conclusion Bushen Shengxue Jiedu Fang may have curative effect on AA through increasing EPO activity, inhibiting immune reaction induced by IFNγ, recovering normal function of hematopoietic stem cell, and promoting normal differentiation and mature of red blood cells.
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    Influences of Majie Chuanke Fang on endothelin, nitric oxide and pulmonary artery in rat model of pulmonary heart disease
    LU Yun, ZHANG Hong
    2014, 37 (6):  401-404.  doi: 10.3969/j.issn.1006-2157.2014.06.010
    Abstract ( 416 )   Save
    Objective To observe the influences of Majie Chuanke Fang on endothelin (ET), nitric oxide (NO) and pulmonary artery in rat model of pulmonary heart disease, and discuss the therapeutic mechanism of Majie Chuanke Fang in treatment of pulmonary heart disease. Methods SD rats (n=56) were randomly divided into high-dose, mid-dose and low-dose Majie Chuanke Fang groups (high-dose group, mid-dose group and low-dose group), Feilike group, captopril group, model group and normal group. The rat model of pulmonary heart disease was established with monocrotaline. High-dose group, mid-dose group and low-dose group were given Majie Chuanke Fang in doses of 12 g/kg, 6 g/kg and 3 g/kg respectively, Feilike group, Feilike Capsules (1.08 g/kg) and captopril group, captopril (0.01 mg/kg). After 14 d, the content of NO and ET-1 was detected, and right ventricle hypertrophy index (RVHI), pulmonary artery area and pulmonary artery wall area were calculated,and morphological changes of pulmonary artery were observed in all groups. Results The content of ET-1 decreased significantly in high-dose group, mid-dose group and low-dose group, and NO content increased significantly in mid-dose group and low-dose group. The unbalance of ET-1/NO was ameliorated in high-dose group, mid-dose group and low-dose group (P<0.05 or P<0.01). The pulmonary artery wall area and lumen area were significantly reduced in low-dose group compared with model group (P<0.01). Majie Chuanke Fang had no significant effect on the indexes of right ventricular hypertrophy. The results of histopathologic examination showed that Majie Chuanke Fang reduced pulmonary artery wall thickness and increased lumen area (P<0.01) in varying degrees. Conclusion Majie Chuanke Fang can relieve the sympotoms of acute pulmonary heart disease through alleviating the unbalance of ET-1/NO, right ventricle hypertrophy and pulmonary vessel remodeling.
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    Characteristics and laws of electric signals of dorsal root neuron induced by lifting-thrusting manipulations of acupuncture with different frequency
    CHEN Lei, LIU Yang-yang, GUO Yi, LI Gui-lan, GUO Yong-ming, WANG Jiang, LI Ye-lei
    2014, 37 (6):  405-409.  doi: 10.3969/j.issn.1006-2157.2014.06.011
    Abstract ( 445 )   Save
    Objective To discuss the characteristics and laws of neuron electric signals induced by lifting-thrusting manipulations of acupuncture with different frequency. Methods Healthy and adult SD rats were chosen and anesthetized, and then nerve filaments innervating the region of Zusanli (ST36) were isolated from spinal dorsal root nerve tract L4, and put on bipolar platinum electrode. The lifting-thrusting manipulations of acupuncture with different frequency (50 time/min, 100 time/min, 150 time/min and 200 time/min) were used for stimulating ST36. The discharge of nerve filaments innervating the region of ST36 were recorded, and needling electric signals were analyzed by applying signal sorting after noise reduction for finding out the laws of types and quantity of needling frequency and variation tendency of neuronal discharge. Results Needling electric signals was composed of discharges of 3 types of neurons. As needling frequency increased, the discharge number of the first type of neurons and second type of neurons increased too, but the third type of neurons had no discharge. Conclusion The lifting-thrusting manipulations of acupuncture with different frequency mainly excite two types of neurons. In the frequency ranges of this study, it can be inferred that increased lifting-thrusting frequency will not cause the reaction of neurons that have no responds originally.
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    Influences of different alkaloid penetration enhancers on transdermal absorption of paeoniflorin
    LI Yun, WEI Shu-chang, LIU Yong-qi, YAN Zhi-pan, YAN Chun-lu, ZHANG Jiu-mei, HOU Tao-xia
    2014, 37 (6):  410-413.  doi: 10.3969/j.issn.1006-2157.2014.06.012
    Abstract ( 473 )   Save
    Objective To survey the effects of 3 kinds of penetration enhancers, evodia rutaecarpa alkaloids, sinapine thiocyanate and berberine hydrochloride, on transdermal permeation of paeoniflorin in vitro, screen effective penetration enhancers and improve the transcutaneous permeability of paeoniflorin. Methods The abdomen skin of mice in vitro was taken as permeation barrier. The content of paeoniflorin was determined by using HPLC for studying the influences of 3 kinds of penetration enhancers on kinetic parameters of in vitro transdermal absorption of paeoniflorin including cumulative permeation quantity, transdermal absorption rate and transdermal time-lag within 12 h. Results The transcutaneous permeability of evodia rutaecarpa alkaloids, sinapine thiocyanate and berberine hydrochloride was, respectively, 0.543 9 g/cm2·h, 0.993 0 g/cm2·h and 3.709 9 g/cm2·h, enhancing rate was, respectively, 1.50, 2.74 and 10.22, and transdermal time-lag was, respectively, 2.10 h, 1.12 h and 0.34 h. Conclusion Evodia rutaecarpa alkaloids, sinapine thiocyanate and berberine hydrochloride can significantly improve the transdermal absorption of paeoniflorin.
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    Quality characteristics and correlation analysis of medicinal preparations from Zisu Fang
    AN Qi, TANG Xue-yang, LU Guang-ying, WEN Jing, MEI Ying-ying, ZUO Sheng, ZHU Nai-liang, WANG Chun-mei, ZHOU Yong-qiang, ZHANG Xiao-lei , WANG Yong-yan, SHI Ren-bing
    2014, 37 (6):  414-419.  doi: 10.3969/j.issn.1006-2157.2014.06.013
    Abstract ( 458 )   Save
    Objective To determine the best preparation forms of medicinal in Zisu Fang,and answer the question of how to develop high-quality medicinal. Methods The content of baicalin,salvianolic acid B,rosmarinic acid,tanshinone ⅡA,matrine,oxymatrin﹙effective index components in different medicinal preparation forms from Zisu Fang﹚were detected by using HPLC-PDA under acidic and alkaline systems.The quality of medicinal preparation from Zisu Fang were characterized based on the content,relative quantity,relative ratio of content,yield rate and antibacterial effect in vitro and in vivo,and the characterized results were given correlation analysis. Results The content of 6 kinds of effective index component﹙totally 53.43%﹚in enrichment from Zisu Fang increased significantly,which was 3.2 times higher than in ethanol extract and 3.6 times higher than in water extract,and the yield rate decreased by one-third of that of ethanol extract and water extract.The relative ratio of components was more reasonable,and medicinal system had higher antibacterial effect in vitro and in vivo.The enrichment was the best preparation form of medicinal in Zisu Fang. Conclusion It is an important way for develop high-quality medicinal to explore the fittest content and relative ratio of medicinal components based on the basic composition of medicinal system and yield rate.
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    Curative effect of needling in 3 acupoints around eye and Fengchi (GB20) on optic atrophy
    YAN Xiao-ling, WEI Qi-ping, LI Li, ZHOU Jian
    2014, 37 (6):  420-423.  doi: 10.3969/j.issn.1006-2157.2014.06.014
    Abstract ( 541 )   Save
    Objective To study the curative effect of needling in 3 acupoints around eye [Jingming (BL1), Chengqi (ST1) and Shangming] and Fengchi (GB20) on optic atrophy. Methods The patients (n=40) were randomly divided into treatment group and control group (each n=20) by applying prospective randomized controlled method. Control group was given Chinese medicinal decoction and support therapy of nerve nutrition, and treatment group was given acupuncture in Jingming (BL1), Chengqi (ST1), Shangming and Fengchi (GB20) once a day besides of the same treatment as control group. After 4 w, the changes of vision, visual field and visual evoked potential (VEP) were observed in two groups. Results The total effective rate was 81% in treatment group and 52% in control group. After treatment and in treatment group, vision was significantly improved, mean light sensitivity (MS) increased significantly, mean defect (MD) decreased significantly and evoked potential amplitude increased significantly(P<0.05). Conclusion Needling in 3 acupoints around eye and Fengchi (GB20) has good curative effect on optic atrophy.
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    Correlation between gene polymorphism of estrogen receptor-a and hypomenorrhea
    LIN Xiao-hua, JIA Yun-bo, JIN Zhe, TAN Zhan-wang
    2014, 37 (6):  424-428.  doi: 10.3969/j.issn.1006-2157.2014.06.015
    Abstract ( 412 )   Save
    Objective To investigate the distribution of gene polymorphism of PvuII and XbaI in gene 1 of estrogen receptor-a (ERa) in patients with hypomenorrhea. Methods The gene polymorphism of ERa in peripheral blood was detected by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in experimental group and normal group. The distribution difference in genotypes of PvuⅡ and XbaⅠ and allele count were compared between two groups. Results The frequency distribution of genotypes of PP, Pp and pp of ERa PvuII and genotypes of XX, Xx and xx XbaI had significant difference between two groups (P<0.05). The constituent ratio of genotypes in experimental group was orderly as follows:PP:5.45%,Pp:40.00%,pp:54.55%,XX:1.82%,Xx:36.36%,and xx:61.82%. The constituent ratio of genotypes in normal group was orderly as follows:PP:18.18%,Pp:40.91%,pp:40.91%,XX:9.09%,Xx:50.00%,and xx:40.91%. The frequency distribution of allele P, p and X, x had significant difference between two groups (P<0.05). The risk of hypomenorrhea of allele P was 0.55 times higher than allele p, and allele X was 0.483 times higher than allele x. Conclusion There is correlation between gene polymorphism of PvuII and XbaI of Era and hypomenorrhea. Allele p and allele x may be the risk factors, and genotype pp and genotyme xx may be susceptible to hypomenorrhea.
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    Cited papers by CSCD on Journal of Beijing University of Traditional Chinese Medicine from 2006 to 2012
    ZHANG Chi, YOU Hai-yan, LI Yan, LU Guang-lin, LIANG Ji-chun
    2014, 37 (6):  429-432.  doi: 10.3969/j.issn.1006-2157.2014.06.016
    Abstract ( 436 )   Save
    Through collecting the cited data of issued papers on Journal of Beijing University of Traditional Chinese Medicine (JBUTCM) by Chinese Science Citation Database (CSCD) from 2006 to 2012, the annual cited paper quantity, cited rate and total cited frequency of JBUTCM were summed up and analyzed. The issued papers with top annual cited rate, amount of other journals cited papers on JBUTCM and involved discipline fields were abstracted for summing up experience and providing reference to develop high-level journal.
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