Influences of Danggui Buxue Tang on function of circulating endothelial progenitor cells and Bcl-2 expression
2014, 37 (6):
Objective To observe the influences of Danggui Buxue Tang on function of circulating endothelial progenitor cells (EPCs) and Bcl-2 expression. Methods Human mononuclear cells in peripheral blood were isolated and cultured for 7 d. After adherent cells identified, EPCs were randomly divided into 6 groups: blank group, control group, simvastatin group, and high-dose, mid-dose and low-dose Danggui Buxue Tang groups (high-dose group, mid-dose group and low-dose group). Blank group was cultured with nutrient solution without medicated serum, control group, 0.2% bovine serum albumin, simvastatin group, 0.1 μmol/L simvastatin, and high-dose group, mid-dose group and low-dose group, respectively, 4 g, 2 g and 1 g Danggui Buxue Tang. The supermatant was eliminated 24 h after culturing, and then all groups were given nutrient solution without medicated serum for continuous culturing for 24 h. The proliferation ability, adhesive force, migration ability and tubule formation of EPCs were detected, respectively, by using MTT, adhesion test, Transwell assay and in vitro angiogenesis kit. The expressions of Bcl-2 mRNA and Bcl-2 protein were detected respectively by using RT-PCR and Western blot. Results All functions of EPCs were significantly improved and expression of Bcl-2 mRNA was up-regulated in high-dose group and simvastatin group (P<0.05). The proliferation, migration of and tubule formation of EPCs were improved but adhesive force and expression of Bcl-2 mRNA were not affected in mid-dose group and low-dose group. The expression of Bcl-2 protein was up-regulated in high-dose group, mid-dose group, low-dose group and simvastatin group (P<0.05). Conclusion Danggui Buxue Tang can improve the function of EPCs through up-regulating the expressions of Bcl-2.
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