Panax notoginseng saponins promote proliferation and differentiation of CD105+-BMSC homing to cerebral infarction marginal zone
2014, 37 (11):
Objective To investigate the promotion effect of panax notoginseng saponins (PNS) on CD105+-bone mesenchymal stem cells (BMSC) homing to the cerebral infarction marginal zone. Methods The rat model of MCAO was established by referencing Zea-Longa method. The modeled rats were randomly divided into model group and high-dose ,mid-dose and low-dose PNS group(high-dose group, mid-dose group and low-dose group), and then according to time points divided into 1 d,3 d,7 d,14 d and 28 d subgroups. High-dose group, mid-dose group and low-dose group were treated with Xuesaitong Capsules (60, 40 and 20 g/L once a day), and normal group and model group were orally administered saline water once a day for 28 d. The positive cells of CD105+-BMSC, MAP-2, GFAP and IIIβ-Tubulin were detected by immunohistochemical method at different time points. Results The positive cells of CD105+-BMSC, MAP-2, GFAP and IIIβ-Tubulin increased in model group and PNS groups on the first day, reached the peak on the 7th day, and then went down gradually. The positive cells of CD105+-BMSC, Map-2, GFAP and IIIβ-Tubulin in model group and all PNS groups compared with model group increased significantly (P＜0.05). Conclusion PNS can repair the damaged cerebral tissue induced by infarction by promoting the proliferation and differentiation of BMSCs homing to infarction marginal zone.
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