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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 November 2014, Volume 37 Issue 11 Previous Issue    Next Issue
    Mental image theory of Chinese traditional philosophies applied to traditional Chinese medicine (Ⅰ): interpretation of mental image theory
    FAN Yi-pin, ZHANG Zhi-bin, WANG Yong-yan
    2014, 37 (11):  725-728.  doi: 10.3969/j.issn.1006-2157.2014.11.001
    Abstract ( 472 )   Save
    Mental image, one of key concepts in Chinese traditional philosophies, is the mode of TCM original thinking. Through interpretation under the embryology viewpoint and academic connotation, mental image is revealed to be rooted in philosophy of mind of Chinese traditional philosophies. Mental image is foremost defined as the form of representation, presented in the form of mind, spirit, emotion, dream, void-quietness, noumenon, and so on.
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    Characteristics of Chinese medicine syndromes and syndrome elements on hypertension based on literature
    QI Yi-xin, XIE Yan-ming, LI Yuan-yuan
    2014, 37 (11):  732-735.  doi: 10.3969/j.issn.1006-2157.2014.11.003
    Abstract ( 410 )   Save
    Objective To investigate the rule of TCM syndromes, combination and distribution of syndrome elements on hypertension, so as to provide a basis for standardization of TCM research on hypertension. Methods After search the literature of TCM treatment of hypertension from 1986 to 2013, database was established by Note Express software, followed by frequency and rate of the syndrome elements were collected and calculated by SPSS 12.0 statistical software. Results Altogether 91 895 cases and 57 Chinese medicine syndromes were involved in 3 986 articles. The main syndromes were ranked as following: hyperactivity of liver-yang (25.32%), hyperactive liver fire (18.07%), phlegm turbidity obstruction (8.23%), liver-kidney yin deficiency (6.99%), deficiency of yin and yang(6.94%), kidney qi deficiency (5.89%). And syndrome elements were distributed into excess syndrome (yang hyperactivity,internal heat, phlegm turbidity,liver wind, blood stasis and liver qi stagnation) and deficiency syndrome(yin deficiency and qi deficiency). Affecting targets of syndrome elements located in liver, kidney and heart. Conclusion In view of complicated TCM diagnosis principles of hypertension and clinical syndromes, the study of syndrome and syndrome elements is crucial to standardization of TCM differentiation and treatment of hypertension.
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    Correlation between syndrome differentiation of yin-yang and inflammatory markers in patients with severe sepsis and septic shock
    MAI Shu-tao, WENG Yan-na, DU Jiong-dong, LAI Fang, HAN Yun
    2014, 37 (11):  736-739.  doi: 10.3969/j.issn.1006-2157.2014.11.004
    Abstract ( 416 )   Save
    Objective To investigate the distribution of syndrome differentiation of yin-yang in severe sepsis and septic shock patients, and to study correlation between yin syndrome or yang syndrome with inflammatory markers. Methods Based on the principles of TCM syndrome differentiation, 98 of 208 patients with severe sepsis and septic shock were diagnosed as yin syndrome, and 110 patients as yang syndrome. Acute physiology and chronic health evaluation Ⅱ(APACHEⅡ) score and systemic inflammatory response syndrome (SIRS) score were recorded, as well as 28 d mortality. Lactic acid(LAC), WBC, percentage of neutrophils (NE%), C-reactive protein (CRP) and procalcitonin (PCT) were determined. Results Yin syndrome patients with mean SIRS score 2.51±0.62 were significantly lower than yang syndrome patients with 3.06±0.80(P<0.05). However, PCT in yin syndrome patients were significantly higher than those in yang syndrome patients(P <0.05). No statistically differences were showed in APACHEⅡ score, WBC, NE% and CRT. In yin syndrome patients, 72 of 98 were survival (survival group)and 26 were dead(death group). APACHEⅡscore and NE% in survival group with yin syndrome were significantly lower than death group(P<0.05). APACHEⅡ score and PCT in survival group (n=81) with yang syndrome were significantly lower than death group (n=29)(P<0.05). Conclusion Syndrome differentiation of yin-yang can be used in predicting the inflammatory state and prognosis in patients with severe sepsis and septic shock.
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    Characteristics of nonlinear chaos between excess syndrome and deficiency syndrome of CHD patients
    YANG Yang, LI Fang-jie, LI Xia, YANG Xin-chun, ZENG Hui, DAI Yue-long
    2014, 37 (11):  740-742.  doi: 10.3969/j.issn.1006-2157.2014.11.005
    Abstract ( 485 )   Save
    Objective To observe the nonlinear parameters in excess syndrome and deficiency syndrome in patients with coronary heart disease (CHD), so as to explore the characteristics of nonlinear chaos existed in TCM syndromes. Methods Based on principles of TCM syndrome differentiation,44 of 243 patients with CHD were diagnosed as excess syndrome(excess group),148 as mingled syndrome(mingled group) and 51 as deficiency syndrome(deficiency group). Each patient underwent 24 h dynamic electrocardiogram (Holter). Correlation dimention(CD) , complexity(Comp) and approximate entropy(ApEn)were calculated relied on the data obtained from Holter to find out the difference of these parameters in all three groups. Results Except for CD in excess group was less than mingled group, the values of CD, Comp and ApEn had a feature in common with the highest in excess group, medium in mingled group and lowest in deficiency group. Conclusion Nonlinear analysis, as a new methodology, can be introduced into TCM syndrome differentiation
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    Characteristics of meridians and acupoints selection for treatment of simple obesity: a data mining research on latest 10 years
    WANG Ju-ju, CHEN Xia, YU Mei-ling, LU Sheng-feng
    2014, 37 (11):  743-747.  doi: 10.3969/j.issn.1006-2157.2014.11.006
    Abstract ( 487 )   Save
    Objective To reveal the rules of selection and matching meridians and acupoints for simple obesity on the basis of data mining. Methods All data were collected from literatures in CNKI regarding to acupuncture treatment of simple obesity during the latest 10 years, so as to establish a prescription database for simple obesity. The descriptive analysis, association rules analysis and clustering analysis were conducted by SPSS 17.0 and Clementine 12.0 software. Results The leading three selection of meridians were Stomach meridian, Ren meridian and Spleen meridian, and the leading acupoints were Zhongwan(CV12),Zusanli(ST36),Fenglong(ST40),Sanyinjiao(SP6) and Tianshu(ST25). The association rules analysis showed that Quchi(LI11), Fenglong(ST40), Qihai(CV6) and Zhongwan(CV12) had the highest correlation among all the selected acupoints. The clustering analysis figured out altogether 9 core clustering groups and two kinds of acupoints, comprised of special acupoints which were the most common choice and specific acupoints which were selected by syndrome differentiation. Conclusion The rules of meridians and acupoints selection can guide acupuncture treatment of simple obesity.
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    Relationship between objective cognitive impairment and liver depression syndrome in patients with subjective memory complaints: a cross-sectional study
    DAI Zhong, WANG Shao-jie, BAI Wen, LIU Hong-sheng, CHEN Fu-qin, YU Hai-mo, LIU Qiang, JIN Xiang-lan, XUE Pei-ran, ZHANG Yun-ling
    2014, 37 (11):  748-752.  doi: 10.3969/j.issn.1006-2157.2014.11.007
    Abstract ( 417 )   Save
    Objective To study the relationship among subjective memory complaints (SMC) , objective cognitive impairment, depression, and liver depression syndrome. Methods Subjects were comprised of two groups: SMC group (n=95) and control group without SMC (n=19). Subjective memory complaints were assessed by subjective memory complaints visual analog Scale (SMC-VAS). Objective cognitive were examined by mini-mental state examination (MMSE) and Montreal cognitive assessment (MoCA). Depression was evaluated by Hamilton depression rating scale (HAMD). And the scores of liver depression syndrome were calculated. Then, the regression analysis of factors on SMC were conducted. Results MMSE and MoCA scores in SMC group were lower than control group(P<0.05), while HAMD score was higher(P<0.05). The scores of liver depression syndrome in SMC group were higher than control group(P<0.05).Logistic regression analysis showed that lower MMSE and higher HAMD scores predicted more incidence of SMC. Linear regression analysis indicated that lower MMSE scores, higher HAMD scores, first-degree relatives with dementia and living alone all predicted worse memory self-assessment. Conclusion SMC is closely allied to objective cognitive and depression. Liver depression syndrome is connected with subjective or objective cognitive impairment. Liver qi stagnation may be the main pathogenesis of SMC in TCM.
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    Effects of Feixian Formula on vascular endothelial growth factor (VEGF) and VEGF receptor 1 and 2 mRNA expression in rat model of interstitial pulmonary fibrosis
    LIU Zhe, ZHANG Xiao-mei, QIN Hui-hui, ZHANG Li-yuan, CHEN Liang, HUANG Yan-fang, WANG Qi, GUAN Li-zhen
    2014, 37 (11):  753-757.  doi: 10.3969/j.issn.1006-2157.2014.11.008
    Abstract ( 778 )   Save
    Objective To investigate the inhabitation effects of Feixian Formula on angiogenesis in rat with interstitial pulmonary fibrosis. Methods Male Wistar rats were randomly divided into 7 groups: sham-operation group, model group, 3 groups treated with high-,mid-,and low-dose of Feixian Formula (high-dose group, mid-dose group and low-dose group), and 2 groups treated with western medicine losartan and prednisone respectively(losartan group and prednisone group). All the rats, except for those in sham-operation group, were established as the model of interstitial pulmonary fibrosis by perfusion of bleomycin (3 mg/kg) through tracheal intubation. From the second day after modeling, all rats were intragastrically administrated with drugs or saline, according to different groups designed. The rats were sacrificed at the 14th day and at the 28th day. The pathological changes of interstitial pulmonary by HE staining and MASSON staining were observed, to evaluate the degree of the alveolitis and pulmonary fibrosis. Expressions of VEGF, VEGF 1 and 2 mRNA were detected by RT-PCR. Results Both high-dose and mid-dose Feixian Formula had obvious effects of inhabiting alveolitis and pulmonary fibrosis , as well as downregualting the expressions of VEGF, VEGFR1 and 2 mRNA, which were remarkable in mide-dose group compared with model group (P<0.05). Conclusion The effects of Feixian Formula on inhabition of angiogenesis and resistance to interstitial pulmonary fibrosis may be involved in the down-regulation of VEGF, VEGFR1 and VEGFR2 gene expression.
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    Effects of ginsenoside Rg1 on Wnt signal pathway of cardiac fibroblasts cultured in high concentration glucose
    HAN Lu-tuo, REN Jun-guo, YANG Jia-mei, LIU Jian-xun
    2014, 37 (11):  758-762.  doi: 10.3969/j.issn.1006-2157.2014.11.009
    Abstract ( 467 )   Save
    Objective To observe the effects of ginsenoside Rg1 on cardiac fibroblasts (CFs) proliferation, protein secretion and Wnt signal pathway, followed the establishment of CFs proliferation model in vitro induced by high concentration of glucose. Methods Cell proliferation was detected by MTT method, cell cycle was measured by flow cytometry, concentration of type I, III collagens and TGF-β 1 protein were tested by ELISA assay. Protein expression of β-catenin,GSK-3β and P-GSK-3β were determined by Western blot,aimed to investigate the effects of ginsenoside Rg1 on Wnt signal pathway. Results Ginsenoside Rg1 inhibited CFs proliferation(P<0.01), reduced type I and III collagens secretion(P<0.05) and synthesis of TGF- β1(P<0.01). Expression of β-catenin was inhibited and expression of P-GSK-3β was upregulated at the molecular level. Conclusion Ginsenoside Rg1 can relieve myocardial fibrosis, due to inhibition of proliferation, secretion of collagens and TGF-β 1 in CFs which were cultured in high concentration of glucose, leading to inhibition of abnormal expression of Wnt signal pathway.
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    Effects of ethyl acetate extract from Cynomorim songaricum Rupr. on learning and memory function and hippocampal neurons in ovariectomized rat model with Alzheimer's disease
    TIAN Fang-ze, CHANG Hong-sheng, ZHOU Jing-yang , LU Yi
    2014, 37 (11):  763-766.  doi: 10.3969/j.issn.1006-2157.2014.11.010
    Abstract ( 452 )   Save
    Objective To investigate the effects of ethyl acetate extract of Cynomorim songaricum Rupr. (Suoyang) on behaviors and synapse in ovariectomized rats. Methods SD rats(n=40) were randomly divided into normal group, model group, estradiol group and ethyl acetate extract of Suoyang (Suoyang group). Alzheimer's disease model was established by ovariectomization. One week after surgery, estradiol group and Suoyang group were intragastrically administered with estradiol and ethyl acetate extract of Suoyang(33 mg/kg)respectively for 8 consecutive weeks. After 6 days’ behavioral observation by Morris water maze, specimen were collected from the hippocampal area. HE staining, immunohistochemistry reaction of growth associated protein(GAP-43) and ultra-micro perspective electron microscopy were used to observe hippocampal neurons. Results Compared with model group, stage of latency was significantly shorter and the times of passing platform was significantly more in Suoyang group(P<0.01). The neurological changes of pyramidal cells in CA3 zone of hippocampus were ameliorated, and the expression of GAP-43 increased significantly(P<0.01). Conclusion Ethyl acetate extract of Suoyang can improve the learning and memory ability in ovariectomized rat model with Alzheimer's disease, attributed to increasing the content of GAP-43 in hippocampus and the formation of synapse in brain.
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    Effects and mechanism of peppermint oil on membrane fluidity and potentials of HaCaT keratinocytes
    LAN Yi, CHEN Yan-yan, ZHAO Bo-chen, LIU Na, WANG Wen-ping, ZHANG Qing, WU Qing
    2014, 37 (11):  767-772.  doi: 10.3969/j.issn.1006-2157.2014.11.011
    Abstract ( 505 )   Save
    Objective To observe the effects of peppermint oil on membrane fluidity and membrane potentials of HaCaT keratinocytes, so as to reveal the potential mechanism of enhancing transdermal penetration. Methods The cytotoxicity of peppermint oil was evaluated by using cell counting kit (CCK-8) assay. The fluorescence recovery after photobleaching (FRAP) technique was adopted to measure the change of membrane fluidity of HaCaT cells. The flow cytometer was used to study the alteration of membrane potentials of HaCaT cells, and to determine the intracellular concentration of Ca2+. Ca2+-ATPase activity in HaCaT cells was detected by using Ca2+-ATPase Kit. Results Compared with azone, a common chemical penetration enhancer, peppermint oil extracted from Mentha haplocalyx Briq. had lower cytotoxicity. Peppermint oil increased HaCaT cells membrane fluidity and decreased membrane potentials. Meanwhile, the intracellular concentration of Ca2+ was increased significantly, and the Ca2+-ATPase activity was decreased (P<0.05). Conclusion Peppermint oil influenced the membrane fluidity and potentials of cells by breaking the Ca2+ balance of the inner and outer membrane, leading to the higher skin permeability to enhance the transdermal absorption of drugs.
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    Effects on P21WAF1 and CyclinD1 in chronic atrophic gastritis with dysplasia in rats: TCM therapy of replenishing qi, resolving stasis and detoxification
    HE Mei-juan , YANG Jin-xiang, AN Jing, HUANG Da-wei, JIA Yun-fei
    2014, 37 (11):  773-776.  doi: 10.3969/j.issn.1006-2157.2014.11.012
    Abstract ( 435 )   Save
    Objective To observe the effects of Xiaopi granule on gene P21WAF1 and gene CyclinD1, which was formulated by replenishing qi, resolving stasis and detoxification, in chronic atrophic gastritis with dysplasia in rats. Methods 50 of 60 Wistar rats were randomly selected to be modeled, and the rest of rats in blank group (n=10). After establishment of model, the alive rats (n=33) were randomly divided into model group, vitacoenzyme tablet group (western medicinal group) and Xiaopi granule group(Chinese medicinal group) (each n=11). Model group, western medicinal group and Chinese medicinal group were intragastrically administered once a day saline water, vitacoenzyme and Xiaopi granule respectively for 12 consecutive weeks. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and Western blot were used to determine the expressions of P21WAF1 and CyclinD1 genes in rats of all groups. Results The expression of P21WAF1mRNA and protein significantly increased in Chinese medicinal group compared with model group and western medicinal group (P<0.05). The expression of CyclinD1 mRNA and protein dramatically decreased in Chinese medicinal group compared with model group and western medicinal group (P<0.05). Conclusion Chinese medicine Xiaopi granule can reverse the gastric mucosal dysplasia, due to increasing the expression of P21WAF1 and inhibition the expression of CyclinD1, leading to redressing the balance of P21-CDK-CyclinD1 pathway.
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    Panax notoginseng saponins promote proliferation and differentiation of CD105+-BMSC homing to cerebral infarction marginal zone
    ZHANG Jin-sheng, ZHANG Bao-xia, ZHU Hui-fang, DU Mei-mei, ZHANG Yang-yang
    2014, 37 (11):  777-780.  doi: 10.3969/j.issn.1006-2157.2014.11.013
    Abstract ( 443 )   Save
    Objective To investigate the promotion effect of panax notoginseng saponins (PNS) on CD105+-bone mesenchymal stem cells (BMSC) homing to the cerebral infarction marginal zone. Methods The rat model of MCAO was established by referencing Zea-Longa method. The modeled rats were randomly divided into model group and high-dose ,mid-dose and low-dose PNS group(high-dose group, mid-dose group and low-dose group), and then according to time points divided into 1 d,3 d,7 d,14 d and 28 d subgroups. High-dose group, mid-dose group and low-dose group were treated with Xuesaitong Capsules (60, 40 and 20 g/L once a day), and normal group and model group were orally administered saline water once a day for 28 d. The positive cells of CD105+-BMSC, MAP-2, GFAP and IIIβ-Tubulin were detected by immunohistochemical method at different time points. Results The positive cells of CD105+-BMSC, MAP-2, GFAP and IIIβ-Tubulin increased in model group and PNS groups on the first day, reached the peak on the 7th day, and then went down gradually. The positive cells of CD105+-BMSC, Map-2, GFAP and IIIβ-Tubulin in model group and all PNS groups compared with model group increased significantly (P<0.05). Conclusion PNS can repair the damaged cerebral tissue induced by infarction by promoting the proliferation and differentiation of BMSCs homing to infarction marginal zone.
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    Sishen pill ameliorates experimental colitis induced by trinitrobenzene sulfonic acid or dextran sulfate sodium in mice
    WANG Xu-dan, YUAN Xue-qin, QIU Ze-ji, GE Dong-yu, LI Gen-mao, HAO Yu
    2014, 37 (11):  781-785.  doi: 10.3969/j.issn.1006-2157.2014.11.014
    Abstract ( 490 )   Save
    Objective To explore the effects of Sishen pill on colitis model induced by trinitrobenzene sulfonic acid (TNBS) or dextran sulfate sodium (DSS) in mice. Methods TNBS colitis model (TNBS colitis)was induced by intrarectally administration of 1.5 mg TNBS in 50% ethanol. DSS colitis model (DSS colitis)was induced by feeding with 4%(w/v) DSS dissolved in drinking water. One day after modeling, mice were intragastically administrated with Sishen pill (2.3 g/kg) for 8 days. Changes in disease activity index (DAI) together with histological scores were evaluated, and myeloperoxidase (MPO) in colonic tissue was detected. Results Sishen pill significantly reduced DAI and histological scores of mice in both TNBS colitis and DSS colitis, it also alleviated inflammatory cell infiltration of colonic tissue in both models. Conclusion Amelioration efficacy of Sishen pill on mice with TNBS colitis and DSS colitis were obtained.
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    Near-infrared spectroscopy for rapid detection of alcohol extract of Jingutongxiao pill
    LEI Jing-wei, ZHANG Qiang, BAI Yan, LI Lei, FAN Ming-yue
    2014, 37 (11):  786-788.  doi: 10.3969/j.issn.1006-2157.2014.11.015
    Abstract ( 468 )   Save
    Objective To determine rapidly the concentration of alcohol extract of Jingutongxiao pill by using near-infrared (NIR) spectroscopy and chemometrics methods. Methods The contents of alcohol extract of 100 batches of Jingutongxiao pill were determined and NIR spectrograms were collected. Partial least squares (PLS) combined with second derivative (SD) was used as spectral processing options. The NIR calibration model of alcohol extracts was established by TQ 8.0 software combined with PLS. Results There were 7 main factors in the quantitative model, whose range was between 8 116.04 and 11 983.82 cm-1. The correlation coefficient of calibration (R2) was 0.99 778, root mean square error of cross validation (RMSEC) was 0.0376, and external prediction deviation(RMSEP)was 0.547. Predicted average relative error was 2.39%. Conclusion The quantitative model established by using near-infrared spectroscopy for determination of alcohol extract of Jingutongxiao pills is rapid, simple and accurate.
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    Application of belt vacuum drying technology on production of Lianhua Qingwen capsule
    WANG Yu-feng, WANG Hua, LI Song, LI Xiang-jun, FAN Wen-cheng, LIU Min-yan
    2014, 37 (11):  789-792.  doi: 10.3969/j.issn.1006-2157.2014.11.016
    Abstract ( 501 )   Save
    Objective In comparation with spray drying technology, to study belt vacuum drying technology on production of Lianhua Qingwen capsule. Methods According to the operating principle of pharmaceutical belt vacuum dryer, altogher six specifications were measured, including flour yield, angle of repose, bulk density, percentage of moisture absorption and the active ingredient contents of forsythiaside A and chlorogenic acid; all those were investigated in spray drying technology parallel. Results The belt drying vacuum technology solved effectively moisture absorption and agglomeration of Lianhua Qingwen capsule, without loss of the active ingredient contents of forsythiaside A and chlorogenic acid. Conclusion Belt vacuum drying technology was suitable for the drying process of industrialization of Lianhua Qingwen capsule.
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