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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 May 2014, Volume 37 Issue 5 Previous Issue    Next Issue
    Guiding principles for studying the language of chinese medicine based on metaphorical cognition
    JIA Chun-hua
    2014, 37 (5):  293-296.  doi: 10.3969/j.issn.1006-2157.2014.05.001
    Abstract ( 472 )   Save
    The language of Chinese medicine described a metaphorical world of human physiology, pathology, diseases and treatment. To understand this metaphorical world, we should study the language of Chinese medicine with operable and effective guiding principles. The principles established based on the metaphorical cognition are as follows: What kind of the Chinese medicine language is? Who said it? What did language say? Why did the language say so? These principles may lead us to the metaphorical world of human body described by Chinese medicine language.
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    Laws of nature of Chinese herbal medicinal with action of replenishing kidney essence in ancient and modern medical classics
    HE Li-juan, SONG Nan, SUN Yue-jiao, CHU Jie, REN Yan-ling
    2014, 37 (5):  297-299.  doi: 10.3969/j.issn.1006-2157.2014.05.002
    Abstract ( 468 )   Save
    The paper analyzed the species, four qi, five flavors, meridian entry and toxicity of Chinese herbal medicinal with action of replenishing kidney essence in ancient and modern medical classics. The laws of nature of these Chinese medicinal in different periods of history were investigated, and the purpose is to provide theoretical support and literature evidence for clinical administration and development of new resources of Chinese medicinal. The most of these Chinese medicinal are grass, and few are vegetable, fruits and birds. In the aspect o four qi, the most of these Chinese medicinal are warm and normal, and few are heat and cool. In the aspect of five flavors, the most of these Chinese medicinal are sweet, pungent, bitter and salty, and few are sour, astringent and mild. In the aspect of meridian entries, the most of these Chinese medicinal enter meridians of kidney and liver, and few enter meridians of gallbladder and small intestine.
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    Influence of Qingkailing Injection on biofilm of Staphylococcus aureus
    HAN Xue, WANG Yi, WANG Yu-nong, LI Dong, LIU Chong, ZHANG Te-li, WANG Yi-zhu, LIU Jun-feng, HAO Yu
    2014, 37 (5):  300-303.  doi: 10.3969/j.issn.1006-2157.2014.05.003
    Abstract ( 429 )   Save
    Objective To observe the influence of Qingkailing Injection on biofilm of Staphylococcus aureus (S. aureus), and study its antibacterial mechanism. Methods The biofilm of S. aureus was cultured on 96-well plate at formation stage and mature stage. The amount of living S. aureus in biofilm was determined by using Microbial Viability Assay Kit-WST dyeing after OD value obtained from 450 nm by using Microplate Reader. The destructive effect of Qingkailing Injection on measure biofilm was observed by using BioFlux biofilm system, and the changes of mature biofilm structure and S. aureus morphology after medication were observed by using scanning electron microscope. Results Qingkailing Injection reduced the amount of living S. aureus in biofilm at formation stage (22 500 mg/L-352 mg/L), and reduced the amount of the amount of living S. aureus in biofilm at mature stage (22 500 mg/L-1 406 mg/L, P<0.05). The results of BioFlux biofilm system showed that Qingkailing Injection destructed the structure of measure biofilm of S. aureus, and the results of scanning electron microscope showed that Qingkailing Injection induced biofilm loose and S. aureus in different sizes. Conclusion Qingkailing Injection can inhibit the biofilm formation and destruct mature biofilm of S. aureus, so it has the anti-effect on the biofilm of S. aureus.
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    Effective mechanism of iridoid constituents of Zhizhuxiang (Jatamans Valeriana Rhizome) in rats with irritable bowel syndrome
    SHI Rui-rui, WANG Juan, YAN Xing-li, HU Jing-hong, GAO Zeng-ping, JIA Ming-xian, YU Xue, WANG Jing
    2014, 37 (5):  304-308.  doi: 10.3969/j.issn.1006-2157.2014.05.004
    Abstract ( 429 )   Save
    Objective To investigate the influences of iridoid of Zhizhuxiang (Jatamans Valeriana Rhizome) on the changes of 5-hydroxytryptamine (5-HT), tryptophan hydroxylase 1 (TPH1) and monoamine oxidase-A (MAO-A) in rats with irritable bowel syndrome (IBS). Methods Male SD rats were randomly divided into control group, model group, pinaverium group, and high-dose (0.6 mg/kg), mid-dose (0.3 mg/kg) and low-dose (0.15 mg/kg) iridoid groups (high-dose group, mid-dose group and low-dose group). The rat model of IBS was established by using chronic stress method. The influence of iridoid on the threshold of abdominal withdraw reflection (AWR) was observed in all groups. The content of serum 5-HT was detected by using HPLC-EC, the expression of colon TPH1 mRNA was detected by using RT-PCR, and activity of colon MAO-A was determined by using colorimetric method. Results The pain pressure threshold and maximum tolerance pressure threshold decreased significantly in model group (P<0.01), and increased significantly in 3 iridoid groups (P<0.01). In model group, the content of serum 5-HT increased (P<0.05), expression of colon TPH1 mRNA increased (P<0.01) and activity of MAO-A decreased (P<0.05). In mid-dose group and pinaverium group, the content of serum 5-HT and expression of colon TPH1 mRNA decreased significantly and activity of MAO-A increased significantly (P<0.01 or P<0.05). In low-dose group, the expression of colon TPH1 mRNA decreased significantly (P<0.05) and activity of MAO-A increased significantly (P<0.05). Conclusion Iridoid of Zhizhuxiang can affect the content of serum 5-HT and reduce vislral sensitivity in rats with IBS through regulating colon TPH1 and MAO-A.
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    Preventive effect of combined administration of extracts from Danggui (Radix Angelicae Sinensis) on colorectum cancer in mice
    AN Jing, ZHAO Bo-chen, WU Qing, LAN Yi, CHEN Yan-yan, LIU Na, WANG Wen-ping
    2014, 37 (5):  309-313.  doi: 10.3969/j.issn.1006-2157.2014.05.005
    Abstract ( 455 )   Save
    Objective To study the chemopreventive effect of combined administration of angelica polysaccharide (AP) and angelica oil (AO) from Danggui (Radix Angelicae Sinensis) on colorectum cancer in mice induced by azoxymethane/dextran sulfate sodium (AOM/DSS). Methods All mice were divided into model group, AO group, low-dose AP group, high-dose AP group, AO+low-dose AP group, AO+high-dose AP group and blank group. The incidence, size and number of colorectum cancer, and expressions of colorectum PCNA, COX-2 and iNOS were compared among all groups. Results AP and AO all had effects of reducing the incidence of colorectum cancer and inhibiting tumor development, which was the most significant in AO+high-dose AP group (compared with model group, P<0.05). The expressions of PCNA, COX-2 and iNOS decreased significantly in all drug groups (P<0.05 or P<0.01), which was the most significant in AO+high-dose AP group (compared with model group, P<0.05). Conclusion AP and AO all have preventive effect on colorectum cancer and their combined administration have a synergistic effect. The preventive effect of the combined administration of AP and AO on colorectum cancer may be related to they can reduce the expressions of PCNA, COX-2 and iNOS.
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    Effects of Sini San and disassembled formulas of Shenghui Tang on synaptic plasticity
    LIANG Mi, LIN Sen-xiang, MA Jie, SONG Yue-han, SUN Meng-ru, WANG Xing-hua, ZHANG Qi, TAN Yan, LI Feng, HUA Qian
    2014, 37 (5):  314-317.  doi: 10.3969/j.issn.1006-2157.2014.05.006
    Abstract ( 484 )   Save
    Objective To study the effects of Sini San and disassembled formulas of Shenghui Tang on synaptic plasticity. Methods SH-SY5Y was taken as nerve growth model for detecting the effects of Sini San and disassembled formulas of Shenghui Tang in different doses on the proliferation of nervous cells through determining cell viability by using CCK-8 method. PC12 cells were taken as nerve differentiation model for detecting the influences of Sini San and disassembled formulas of Shenghui Tang in different doses on the differentiation of nervous cells through cell morphology observation and determination of the length and differentiation rate of synapsis by using relative statistical analysis. Results Sini San had no significant effect on cell proliferation but had inhibitory effect on cell differentiation. Among disassembled formulas of Shenghui Tang, disassembled Shenghui Tang 4 [Shudi (Radix Rehmanniae Praeparata) and Shanzhuyu (Fructus Corni)] had effects of promoting proliferation and inhibiting differentiation, disassembled Shenghui Tang 6 [Fushen (Poria cum Ligno Hospite) and Hongshen (Radix Ginseng Rubra)] had dual effects of promoting proliferation and differentiation, and disassembled Shenghui Tang 7 [Yuanzhi (Radix Polygalae), Shichangpu (Rhizoma Acori Tatarinowii) and Baijiezi (Semen Sinapis Albae)] had effects of inhibiting proliferation and promoting differentiation. Conclusion Among traditional Chinese medicinal for improving learning and memory ability, those with the actions of soothing liver and regulating qi have effect of inhibiting synaptic plasticity, and those with the actions of dispelling phlegm and boosting intelligence, and actions of tonifying qi and fortifing spleen have the effect of promoting synaptic plasticity.
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    Mismatch negativity in rabbits with acute myocardial ischemia
    YU Qing, CAI Rong-lin, WU Zi-jian, WANG Jie, WANG Chun-hua, LIU Lei, HU Ling
    2014, 37 (5):  318-320.  doi: 10.3969/j.issn.1006-2157.2014.05.007
    Abstract ( 448 )   Save
    Objective To observe the characteristics of latent period, amplitude of mismatch negativity (MMN) and heart rate responses in rabbits with acute myocardial ischemia (AMI), and lay the foundation for further study on heart-brain relation. Methods Healthy adult rabbits (n=20) were chosen and randomly divided into normal group and AMI group (each n=10). The model of AMI was established by using coronary artery ligation. The changes of heart rate were observed by using BIOPAC system, and changes of MMN latent period and amplitude were recorded by using American NEUROSCAN ERPs. Results After AMI model was established, heart rate decreased significantly (P<0.01). MMN latent period was extended and MMN amplitude decreased in AMI group compared with normal group (all P<0.01). Conclusion AMI can impact heart function, reduce cerebral ability of treating information and influence cognitive function.
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    Fumigation and intratracheal instillation of lipopolysaccharide or they combining ozone exposure for establishing COPD models in rats
    LI Si-wei, ZHANG Ling, LI Chun-lei, LI You-lin
    2014, 37 (5):  321-324.  doi: 10.3969/j.issn.1006-2157.2014.05.008
    Abstract ( 456 )   Save
    Objective To review rat COPD models established by using fumigation+intratracheal instillation of lipopolysaccharide (LPS) or fumigation+LPS combining ozone exposure. Methods Male SD rats (n=30) were randomly divided into blank group, model I group and model II group. The general condition and symptoms of COPD were observed in rats. The morphological changes of lung tissue and inflammatory cell infiltration were observed after HE staining, and results of mean lung interval (MLI) and mean alveoli number (MAN) were observed and analyzed after HE staining. Results COPD models established by using two methods was satisfactory. The detection of lung function showed that central airway resistance (Rn) and organization damping (G) increased in model I group and model II group compared with blank group (P<0.05), which indicated there were airway limitation and ventilation dysfunction in model I group and model II group. The swelling, hyperplasia and exfoliation of all levels of bronchial mucosal epithelium, serious inflammatory cell infiltration in airway and lung tissue, thickened of walls of airway and pulmonary arterioles and bullae of lung were observed after HE staining. All of these pathological changes were more serious in model II group compared with model I group. The results of MLI and NAN showed that MAN was lower and MLI was higher within unit area in model I group and model II group than that in blank group P<0.05). Conclusion Fumigation and intratracheal instillation of lipopolysaccharide or they combining ozone exposure can reappear the pathological features of COPD and establish successfully rat COPD models. After combining ozone exposure, the pathological damages in lung are more serious, which is more approximate to clinical situation.
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    Influences of Shugan Jiangu Fang combining anastrozole on expressions of BMP-2 mRNA/protein and Runx2 mRNA/protein in MG-63 cells
    LI Yuan-qing, HAN Shu-yan, LI Ping-ping
    2014, 37 (5):  325-329.  doi: 10.3969/j.issn.1006-2157.2014.05.009
    Abstract ( 434 )   Save
    Objective To study the influences of Shugan Jiangu Fang on genetic expressions and protein synthesis of bone morphogenetic protein 2 (BMP-2) and Runx2 in MG-63 cells, and discuss its mechanism of influencing bone anabolism. Methods MG-63 cells were cultured and passaged in vitro, and divided into four groups and intervened with different drugs: control group, anastrozole group (ANA group), Shugan Jiangu Fang group and combining group. After cultured for 48 h, the total RNA was extracted from MG-63 cells. The expressions of BMP-2 mRNA and Runx2 mRNA were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). After extracting the total cellular protein, the changes of protein synthesis of BMP-2 and Runx2 were detected by using Western blotting. Results The expressions of BMP-2 mRNA and Runx2 mRNA were up-regulated in combining group and Shugan Jiangu Fang group. The relative expression of BMP-2 mRNA was 1.65 times higher in combining group and 2.34 times higher in Shugan Jiangu Fang group than that in control group, and relative expression of Runx2 mRNA was 1.41 times higher in combining group and 2.33 times higher in Shugan Jiangu Fang group than that in control group. The expressions of BMP-2 mRNA and Runx2 mRNA were down-regulated in ANA group. The expressions of BMP-2 protein and Runx2 protein increased in combining group and Shugan Jiangu Fang group. The relative expression of BMP-2 protein was 0.75 in combining group and 1.01 in Shugan Jiangu Fang group, and relative expression of Runx2 protein was 0.07 in combining group and 0.12 in Shugan Jiangu Fang group. The expressions of BMP-2 protein and Runx2 protein decreased in ANA group and had no significance compared with control group but had significance compared with combining group and Shugan Jiangu Fang group. Conclusion Shugan Jiangu Fang has the effect of promoting bone anabolism, which may be related to that it can up-regulate the genetic and protein expression of BMP-2 and Runx2.
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    Influences of Bushen Huoluo Fang on sex hormones and ovarian morphology in rats with polycystic ovary syndrome
    LIN Hui, HUANG Qun, WAN Ping, YANG Yan, FU Feng-chun, PENG Gui-yang
    2014, 37 (5):  330-332.  doi: 10.3969/j.issn.1006-2157.2014.05.010
    Abstract ( 417 )   Save
    Objective To investigate the pharmacologic mechanism of Bushen Huoluo Fang in the treatment of polycystic ovary syndrome (PCOS). Methods Female SD rats (n=60) were randomly divided into blank group, model group, positive drug group, and high-dose, mid-dose and low-dose Bushen Huoluo Fang groups (high-dose, mid-dose and low-dose groups, each n=10). The model of PCOS was established by using insulin combining human chorionic gonadotropin (HCG). Bushen Huoluo Fang (in three doses) and Diane-35 were administrated in corresponding groups respectively for 12 d, and then the levels of serum luteinizing hormone (LH), testosterone (T), follicle-stimulating hormone (FSH) and estradiol (E2) were detected by using radioimmunoassay, and the changes of ovarian morphology were observed with light microscope. Results Compared with model group, the weight of ovary (P<0.05), follicle amount (P<0.05) and levels of serum T and LH (P<0.05) decreased significantly, and histomorphology changes were improved in high-dose and mid-dose groups. Conclusion Bushen Huoluo Fang can promote follicular development and ovulation through reducing the levels of T and LH.
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    Influences of Chuankezhi Injection on Th17 and Treg in mice with airway inflammation
    HUANG Dong-hui, WANG Hui-xian, WU Shao-li, LI Tan-hui
    2014, 37 (5):  333-336.  doi: 10.3969/j.issn.1006-2157.2014.05.011
    Abstract ( 384 )   Save
    Objective To investigate initially the influences of Chuankezhi Injection on Th17 and Treg in mice with asthma airway inflammation. Methods The model of bronchial asthma was established by applying the intraperitoneal injection of OVA combining nasal drop in BALB/c mice, and then randomly divided into control group, asthma group and Chuankezhi group. Chuankezhi group was intraperitonealy injected Chuankezhi Injection (1.04 ml/kg) and control group and asthma group were intraperitonealy injected normal saline water (200 UL). The changes of lung pathology, levels of BALF IL-17, IL-6, TGF-β1 and IL-10, percentage of CD4+IL-17+ and FOXp3+CD4+CD25+ in peripheral blood, and expressions of lung Foxp3 mRNA, RORγt mRNA and IL-17 mRNA were observed in mice of all groups. Results The pathologic sections of lung tissue showed that there were infiltration of lot eosinophils, lymphocytes and other inflammatory cells around trachea in asthma group, which indicated that model establishment was successful. The level of IL-6 was lower and level of TGF-β1 was higher in Chuankezhi group than those in asthma group (P<0.05). The percentage of CD4+IL-17+ was down-regulated and that of FOXp3+CD4+CD25+ was up-regulated in Chuankezhi group, which was opposite in asthma group (P<0.05). The expression of Foxp3 mRNA was higher and expressions of IL-17 mRNA and RORγt mRNA were lower in Chuankezhi group than those in asthma group (P<0.05). Conclusion Chuankezhi Injection may inhibit the airway infective reaction through reducing the generation of regulatory T cells of FOXp3+ and inhibiting IL-17 release.
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    Quality-representation of medicinal-preparation of Lianqiao Yin based on medicinal system
    MEI Ying-ying, XU Shu-ya, ZUO Sheng, PENG Ping, QUAN Jian-ye, AN Qi, JIANG Yan-yan, WANG Chun-mei, WANG Yong-yan, SHI Ren-bing
    2014, 37 (5):  337-343.  doi: 10.3969/j.issn.1006-2157.2014.05.012
    Abstract ( 458 )   Save
    Objective To establish the method of quantitative determination for effective index components of medicinal-preparation of Lianqiao Yin based on medicinal system, analyze quality-representation correlation, insure key factors influencing quality and prepare antimicrobial agents with high quality from Lianqiao Yin. Methods The content of effective index components of medicinal-preparation of Lianqiao Yin was detected by using HPLC-DAD. According to the size of the antibacterial annulus, use paper disk method to determine the antibacterial effect of drug-preparation in vitro. In vivo, Staphylococcus aureus was cultured for 18 hours and 5% yeast liquor was inoculated into the abdominal cavity of mice who had given medicine. Death rate and average survival time were observed and analyzed within 7days after being infected. Results The effective index components gardenosid(GD); prim-O-glucosylcimifugin(PGCN); cimifugin(CN); liquiritin(LQR); forsythoside A(FTA);4’-O-D-glucosyl-5-O-methylvisamminol(GMV);phillyrin(Phil); glycyrrhizic acid(GZA), of medicinal-preparation of Lianqiao Yin showed a good linear relationship, and qualified average recovery. All 3 kinds of medicinal-preparation had antibacterial effects in vivo and in vitro, and their descending order was as follows: enrichment>alcohol extract>water extract. According to the medicine-efficacy correlation analysis, among 3 medicinal-preparation, the content of effective index components is higher in enrichment with unique proportion, lower extraction rate, lower dosage and higher antibacterial effect, which is the best medicinal-preparation application form of Lianqiao Yin. Conclusion The method is simple, accurate and sensitive with good repetition, which can be used in the study on quality-representation of medicinal-preparation of Lianqiao Yin. Based on the basic composition and extraction rate of medicinal system, the key factor for increasing medicinal efficacy is to promoting relative effective components and optimizing their proportion.
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    Dynamic accumulation laws of total flavonoids and total polysaccharides in different organs of wild and cultivated Gancao (Radix Glycyrrhizae)
    HAN Ya-nan, ZHANG Xue-jing, HOU Jun-ling, WANG Wen-quan, MA Sheng-jun, XIE Jing
    2014, 37 (5):  344-348.  doi: 10.3969/j.issn.1006-2157.2014.05.013
    Abstract ( 470 )   Save
    Objective To reveal the accumulation laws of total flavonoids and total polysaccharides in different organs of Gancao (Radix Glycyrrhizae) at different growth periods. Methods The content of total flavonoids and total polysaccharides in different organs of wild and cultivated Gancao was determined by using ultraviolet-visible spectrophotometry from May to Sept. for finding the accumulation laws. The difference between wild Gancao and cultivated Gancao was analyzed and the accumulation laws were verified with Gancao from different producing areas. Results The accumulation laws of total flavonoids and total polysaccharides were similar in root and leaf of wild Gancao and cultivated Gancao. The content of total flavonoids showed a trend of V type in Gancao root during growth period, and reached the lowest point in full-bloom stage (2.43% in root of wild Gancao and 1.85% in root of cultivated Gancao), and showed a trend of inverted V type in Gancao leaf, and reached the highest point in full-bloom stage (5.64% in leaf of wild Gancao and 6.02% in leaf of cultivated Gancao). In different Gancao organs, the content of total flavonoids was the highest in leaf of wild Gancao and cultivated Gancao in full-bloom stage, but there was difference in Gancao from different producing areas. The change trend of total polysaccharides was complicated in different organs of wild Gancao and cultivated Gancao, which showed a inverted V type (increasing at first and decreasing then). In root of cultivated Gancao, the content of total polysaccharides kept a ever-increase status during the whole growth period, and reached the highest point (18.20%) in fruit-ripening stage. Conclusion Cancao leaf during full-bloom stage can be chosen for studying and utilizing total flavonoids. The content of total polysaccharides is higher in Gancao root in fruit-ripening stage, which is important for the exploitation.
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    Genetic diversity of germplasm resources of Tiepishihu (Dendrobium officinale) analyzed with SRAP
    BAO Ying-hua, PAN Chao-mei, BAI Yin,YAN Yu-ning
    2014, 37 (5):  349-353.  doi: 10.3969/j.issn.1006-2157.2014.05.014
    Abstract ( 442 )   Save
    Objective To analyze the genetic diversity of germplasm resources of Tiepishihu (Dendrobium candidum) from different populations, and provide the experimental data for the protection and sustainable utilization of germplasm resources of Tiepishihu. Methods The DNA bands of 8 populations of Tiepishihu were amplified by using labeling technique of sequence-related amplified polymorphism (SRAP). The genetic diversity was analyzed by using popgene32 software for constructing a cluster dendrogram. Results There were 7 pairs of effective primer combinations screened out from 64 pairs of SRAP primer combinations, and 396 clear bands amplified with 382 polymorphic bands (PPB). The total percentage of PPB was 96.46%, Nei’s genetic diversity (H) was 0.2930 and Shannon’s index (I) was 0.4529. The total index of genetic diversity (Ht) was 0.2930, index of genetic diversity (Hs) within populations was 0.1715, genetic differentiation coefficient (Gst) was 0.4147, and gene flow (Nm) was 0.7057. The clustering analysis showed that 8 populations of Tiepishihu could be classified into 4 types. Conclusion The level of genetic diversity of germplasm resources of Tiepishihu is higher and the degree of genetic differentiation is higher within populations than that among populations.
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    Correlation between syndrome factors of type 2 diabetes and carotid atherosclerosis
    LIU Jing, ZHOU Chun-yu, GUO Rong-juan, CHEN Zhi-gang, WANG Hong-mei, CHEN Bao-xin, ZHAO Hui, SHOU Hao-chang, SHENG Xue, ZHANG Yan, ZHENG Hong, ZHANG Yun-ling
    2014, 37 (5):  354-357.  doi: 10.3969/j.issn.1006-2157.2014.05.015
    Abstract ( 443 )   Save
    Objective To study the correlation between syndrome factors of type 2 diabetes and carotid atherosclerosis. Methods There were 1 452 cases of type 2 diabetes chosen and data of TCM four examinations was collected. The syndrome factors were extracted with factor analysis combining experts’ opinions. The patients were given color Doppler examination of carotid artery. The correlation between syndrome factors of type 2 diabetes and carotid atherosclerosis was studied by using Logistic regression analysis. Results The main syndrome factors of type 2 diabetes were yin deficiency and qi deficiency, secondary factors were yang deficiency, blood stasis and phlegm dampness, and combined factor was excess heat. The disease positions were mainly liver, spleen and kidney. After regulating traditional risk factors, phlegm dampness and blood stasis were significantly correlated to positive rate of carotid atherosclerosis. Conclusion Among the syndrome factors of type 2 diabetes, phlegm dampness and blood stasis may exacerbate the degree of carotid atherosclerosis.
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