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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 September 2014, Volume 37 Issue 9 Previous Issue    Next Issue
    Some hot topics in life nurturing at present
    LIN Yin, JIN Qi, YAN Xing-li, LIAO Yan, ZHANG Cong, ZHANG Qing-yi, ZHANG Yu-ping, ZHANG Yu, YAN Ze, CUI Lian, SHU Xiu-ming, FAN Ning
    2014, 37 (9):  581-585.  doi: 10.3969/j.issn.1006-2157.2014.09.001
    Abstract ( 480 )   Save
    Based on interviews with experts, media research and questionnaire survey, 9 hot debated topics of life nurturing were screened. Even though they are commonly widespread, influential and believed by the public, they lack the academic research and a clear result in their life nurturing value. By adopting interdisciplinary approaches and clarifying the literary lineage and theoretical research, the paper explains the academic, health care and application values of these 9 topics in respect to their inaccuracy, inadequate usage and misconception. For example, mutual restraint between foods and consumption of bear bile for health maintenance are inaccurate, foot massage for curative treatment and excessive consumption of ginseng and Gua Sha treatment for health preservation are inadequate, and tonifying the physique forms with foods with the same forms, eating games and taking a vegetarian diet for health preserving and adjusting the five flavors in accord with seasons are misconception. The principles of life nurturing were confirmed to provide principal concepts and methods for public health preservation including longevity for the benevolent and the wise, Tao Yin to tone the body, balanced in eating and drinking and regular in one’s daily life.
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    Therapy of reinforcing healthy qi and eliminating toxin based on theory of latent toxin for preventing cancer metastasis: a theoretical discussion
    ZHANG Yu-ren, LIN Hong-sheng, ZHANG Ying
    2014, 37 (9):  586-588.  doi: 10.3969/j.issn.1006-2157.2014.09.002
    Abstract ( 485 )   Save
    Cancer stem cells (CSC) exist in tumor tissues and their biologic characteristics of self-renewing and differentiation play a role that cannot be ignored in cancer formation and metastasis. Because cancer has features of insidious onset, easily transferring and poor prognosis, the authors combined TCM theory of latent toxin and characteristics of cancer and CSC, integrated clinical common syndromes of healthy qi efficiency and toxin bind and academic summarization by physicians in all dynasties, and put forward a basic therapeutic principle, therapy of reinforcing healthy qi and eliminating toxin, for preventing cancer metastasis.
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    Correlation between results of TCM constitutional test and present physical fitness test in college students
    WU Zhi-kun, ZHANG Cai, XU Yang-cai
    2014, 37 (9):  598-601.  doi: 10.3969/j.issn.1006-2157.2014.09.006
    Abstract ( 497 )   Save
    Objective To prove the consistency between results of TCM constitutional test and present physical fitness test in college students based on mathematical statistics, and feasibility of bringing TCM constitutional test into the system of physical fitness test in college students. Methods The undergraduates (n=540) were randomly chosen from the Shanghai University of Traditional Chinese Medicine, and their TCM constitutional types were determined by using the Constitution in Chinese Medicine Questionnaire (CCMQ). The tests on body shape, physical function and physical quality were carried out by applying relative instruments. The correlation and consistency between results of TCM constitutional test and present physical fitness test were analyzed by using SPSS13.0 software. Results Members of phlegm-dampness constitution group had higher body mass index(BMI) and those of yin-deficiency constitution had higher body length, which were consistent with constitutional features analyzed by TCM constitution theory. The scores of 9 constitutional types were not significantly correlated to test items of body shape and physical function (P>0.05), and were correlated to some items of physical quality (P<0.05, P<0.01). When score of normal constitutional type was higher, results of all test items of physical quality would be better. The higher the scores of 8 abnormal constitutional types, the poorer the results of all test items of physical quality. Conclusion The results of TCM constitutional test and present physical fitness test in college students are correlated to each other. TCM constitutional test can be brought into the system of physical fitness test in college students for improving the system of physical fitness test.
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    Changes of drug metabolic enzyme CYP3A and transportprotein P-gp in vitro and in vivo in deficiency cold state
    XUE Chun-miao, ZHANG Bing, LI Lian-zhen, LIN Zhi-jian
    2014, 37 (9):  602-605.  doi: 10.3969/j.issn.1006-2157.2014.09.007
    Abstract ( 427 )   Save
    Objective To discuss the changes of drug metabolic enzyme CYP3A and transportprotein P-gp in deficiency cold state through in vitro and in vivo tests. Methods The rat model of deficiency cold was established by injection of hydrocortisone (20 mg/kg) for 14 d. The activity of CYP3A and P-gp level in liver and small intestine were observed in normal and deficiency cold rats. L02 cells and Caco-2 cells were chosen as cell model and intervened with serum of normal and deficiency cold rats for observing the activity of CYP3A in L02 cells and P-gp level in Caco-2 cells. Results The activity of CYP3A decreased significantly in liver and small intestine (P<0.05), and P-gp level decreased significantly in small intestine (P<0.05) in deficiency cold rats. The activity of CYP3A decreased significantly in L02 cells (P<0.05), but P-gp level had no changes in Caco-2 cells in deficiency cold rats. Conclusion The tests in vitro and in vivo have verified that the activity of CYP3A is lower in deficiency cold state. Non-change of P-gp level in Caco-2 cells may be related to that Caco-2 cells are cancer cells and P-gp expression is high.
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    Developable changes of limbic VEGF in rats after stroke and interventional effects of Chinese medicinal
    LI Dong-mei, TANG Qi-sheng, ZHAO Rui-zhen, LI Xiao-li, WANG Ge
    2014, 37 (9):  606-610.  doi: 10.3969/j.issn.1006-2157.2014.09.008
    Abstract ( 447 )   Save
    Objective To investigate the evolution trend of limbic vascular endothelial growth factor (VEGF) and early intervention of Chinese medicinal with the actions of tonifying kidney and regulating qi in rats after stroke. Methods Male Wistar rats were chosen and randomly divided, after open-field ethology scoring, into normal group, sham-operation group, multi-cerebral infarction group (MCI group), Chinese medicinal group and Western medicinal group. The normal group was given routine feeding, sham-operation group was given injection normal saline (0.3 mL) and orally distilled water, and other groups were given injection of allogenic embolus (0.3 mL). The Chinese medicinal group and Western medicinal group were given respectively Chinese medicinal (6.2 g/kg) and nimodipine (9.45 mg/kg) for 4 w after modeling. The changes of limbic VEGF and intervention efficacy of Chinese medicinal were observed by using immunohistochemistry technique on the 1st w, 2nd w and 4th w. Results The outcomes of immunohistochemistry technique showed that positive cells of VEGF increased significantly in MCI group. The expression of VEGF was significantly higher on the 1st w than in other time points in MCI group, and then decreased gradually. The positive cells of VEGF increased significantg in Chinese medicinal group compared with MCI group and normal group on the 2 nd w and 4 th w. Conclusion There are changes of limbic VEGF in rats after cerebrovascular disease, and Yinao Jieyu Fang can improve vascular repair and regeneration.
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    Influences of nanoparticles Rg3 and PEG-PLGA-Rg3 on endothelial cells invasion and tube formation
    GENG Liang, YANG Cai-ling, TIAN Tong-de, YU Jing, SUN Wei-min, JIN Xi-yuan, HUA Bao-jin
    2014, 37 (9):  611-615.  doi: 10.3969/j.issn.1006-2157.2014.09.009
    Abstract ( 421 )   Save
    Objective To observe the influences of ginsenoside Rg3 (Rg3) and PEG-PLGA-Rg3 slow-release nanoparticles (Rg3-N) on endothelial cells invasion and tube formation in vitro, and compared the difference. Methods The influences of Rg3 and PEG-PLGA Rg3-N on endothelial cells invasion and tube formation were observed by using transwell method and tube formation method, and the difference between effects of Rg3 and PEG-PLGA Rg3-N were compared. The gene and protein expressions of E-cadherins (E-cad), matrix metalloproteinase-9 (MMP-9), hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) were detected by using RT-PCR and Western blot method for exploring the internal molecular mechanism. Results The treatment with Rg3 and PEG-PLGA Rg3-N reduced significantly the invasive ability and tube formation of human EA.hy926 endothelial cells (P<0.05), had no significant influences on protein and gene expressions of VEGF, and decreased the expressions of MMP-9 and E-cad. There was no protein expression of HIF-1α observed in all groups. Conclusion The slow-release Rg3 and PEG-PLGA Rg3-N can inhibit the invasive ability and tube formation of human EA.hy926 endothelial cells through inhibiting the expressions of E-cad and MMP-9. PEG-PLGA Rg3-N can improve the antagonism ability to invasion and tube formation of human EA.hy926 endothelial cells in vitro.
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    Influence of therapy of coordinating heart and kidney on vascular endothelial L-Arg/NOS/NO pathway in aged female rats
    XIN Ming-wei, LIU Yan-feng, LUO Bin, WU Ying, JIANG Mei, SUN Tian-lin
    2014, 37 (9):  616-619.  doi: 10.3969/j.issn.1006-2157.2014.09.010
    Abstract ( 446 )   Save
    Objective To investigate the influence of therapy of coordinating heart and kidney on vascular endothelial L-Arg/NOS/NO pathway in aged female rats. Methods Female SD rats (15 months old) were divided into aged group, Chinese medicinal group(20.65 g/kg), Western drug control group(0.12 mg/kg) and Chinese medicinal control group(0.65 g/kg), and respectively given orally normal saline (NS), Chinese medicinal of coordinating heart and kidney, Bujiale and Gengnian’an. Other rats (3 months old) were chosen as young group. The levels of serum estradiol (E2), follicular stimulating hormone (FSH) and luteinizing hormone (LH) were detected by using radioimmunoassay, level of aontic tunica. wdreritita nitric oxide (NO) was detected by using nitrate reductase method and levels of aortic tunica adventitia nitric oxide synthase (NOS) and L-Arg were detected by using colorimetric method. Results Compared with aged group, NOS level had no significant changes in Chinese medicinal group (P>0.05), decrased significantly in Chinese medicinal control group and Western drug control group (P<0.05). Compared with aged group, L-Arg level had no significant changes in Chinese medicinal group and Western drug control group (P>0.05), decreased in Chinese medicinal control group (P<0.05). Compared with aged group, NO level increased in Chinese medicinal group and Western drug control group (P<0.05), and slightly increased in Chinese medicinal control group (P>0.05). The level of estrogen was correlated to NO level. Conclusion The therapy of coordinating heart and kidney can improve estrogen level and has regulating effect on vascular endothelial L-Arg/NOS/NO pathway in aged female rats.
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    Solubilizing effect of glycyrrhizic acid on baicalin
    LU Bing, YANG Xuan, WANG Bao-hua, SHI Xin-yuan, WANG Yan, LI Jie, SHAO Lu, YANG Bei-bei, LI Ping
    2014, 37 (9):  620-624.  doi: 10.3969/j.issn.1006-2157.2014.09.011
    Abstract ( 447 )   Save
    Objective To discuss the mechanism of solubilizing effect of glycyrrhizic acid on baicalin through detecting surface tension and index component of combined decoction of Huangqin (Radix Scutellariae) and Gancao (Radix Glycyrrhizae) in different percentages. Methods The surface tension of the combined decoction in different percentages was detected by using Wihelmy plate method, content of baicalin and glycyrrhizic acid in different proportions was detected by using high performance liquid chromatography (HPLC), and retation graph was completed. Results When the ratio of Huangqin to Gancao was 3:2, the extraction rate of baicalin was the highest, and critical micelle concentration of glycyrrhizic acid was 0.22 g/L. When concentration of glycyrrhizic acid was lower than 0.22 g/L, dissolution rate of baicalin increased gradually as concentration of glycyrrhizic acid increased, ang when concentration of glycyrrhizic acid reached 0.22 g/L, dissolution rate of baicalin did not increase. Conclusion Glycyrrhizic acid has some solubilizing effect on baicalin, and when its critical micelle concentration reaches 0.22 g/L, its solubilizing effect is the higherst.
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    Synthesis of baicalin-zinc complex and its inhibitory effect on human cervical carcinoma HeLa cells
    YUAN Rui-juan, WANG Bei-bei, ZHAO Shuang, SHEN Meng, XU Yi-wen, CHEN Xin, TAN Peng, TAO Ou, LIU Yong-gang
    2014, 37 (9):  625-628.  doi: 10.3969/j.issn.1006-2157.2014.09.012
    Abstract ( 454 )   Save
    Objective To synthesize baicalin-zinc complex, and study its inhibitory effect on human cervical carcinoma HeLa cells. Methods Baicalin-zinc complex was synthesized by reaction between baicalin and zinc acetate, and then the complex was characterized by using ultraviolet-visible absorption spectrum, infrared spectrometry and mass-spectrography. The inhibitory effects of baicalin and baicalin-zinc complex on the proliferation of HeLa cells in vitro were detected by using CCK-8 method. Results Zinc ion (1 molecule) might combine with carboxyl of glucuronic acid (2 molecule) in baicalin to generate baicalin-zinc complex (1:2). The harf inhibitory concentration (IC50) of baicalin-zinc complex and that of baicalin on HeLa cells were, respectively, 12.03 mg/L and 8.69 mg/L. Conclusion Baicalin and baicalin-zinc complex both have anti-tumor activities in vitro, but when baicalin combines zinc to generate complex, the anti-cervical carcinoma activity will decrease.
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    Combination of syndrome factors of vascular mild cognitive impairment based on cluster analysis
    CHEN Bao-xin, JIN Xiang-lan, GUO Rong-juan, CHEN Zhi-gang, GAO Fang, ZHENG Shuo, HOU Xiao-bing, ZHAO Yan, ZHANG Yun-ling
    2014, 37 (9):  629-632.  doi: 10.3969/j.issn.1006-2157.2014.09.013
    Abstract ( 484 )   Save
    Objective To provide reference for studying on TCM syndromes with cluster analysis and factor analysis. Methods On the base of preliminary factor analysis on TCM four examination information of vascular mild cognitive impairment (MCI), the scores of common factors were calculated. An index cluster analysis was conducted after including 24 common factors as variables into cluster analysis for summing up and finding common syndrome types of vascular MCI. Results The common factor of vascular MCI (n=24) were clustered into 5 types, and there were differentiated, based on clinical experience, as syndrome of kidney deficiency, syndrome of internal obstruction of phlegm-heat, syndrome of qi stagnation and blood stasis, syndrome of spleen-kidney deficiency with internal obstruction of phlegm turbidity, and syndrome yin deficiency with effulgent fire. Conclusion The organic integration of factor analysis and cluster analysis can be more objective in the studies on TCM syndromes.
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    Characteristics of neck pain in adolescents
    WU Ji, ZHENG Hao-yun, SHI Zong-ting, SHI Xin-chao, YANG Li-li, ZHAO Jun, ZHANG Dong, SUN Rui-hua, YANG Cun-xia, QI Qing-xue, LANG Song, WANG Qing-fu
    2014, 37 (9):  633-637.  doi: 10.3969/j.issn.1006-2157.2014.09.014
    Abstract ( 415 )   Save
    Objective To investigate the characteristics of neck pain in adolescents. Methods The teenager patients with neck pain (n=1397) were given questionnaire by using clinical epidemic cross-section survey. All data was analyzed taking Northwick Park Neck Pain Scale (NPQ) and McGill Pain Questionnaire (MPQ) as major review indexes. Results In 1397 teenager patients, the average score of NPQ was (12.66±8.61), and average score of MPQ was (4.35±4.88). Among all patients, 93.3% patients could not read optionally due to neck pain, 56.1% had pain symptoms, and 48% had uncomfortable symptoms every day. The most common pain sensations were swelling pain (30.1%), referred pain (29.5%) and sustained fixed pain (25.1%). There were 43.8% patients having cheesed feeling due to neck pain. All neck pain scores were higher in female patients than those in male ones. Conclusion The survey has showed that neck pain in adolescents has characteristics as follows: lower pain degree with adverse influences on life and social activities, higher incidence with intermittent symptoms, swelling pain, referred pain, fixed pain and stabbing pain being main pain types, pain distributing in neck and shoulder, and neck pain correlating to psychological and emotional factors, sex, age and educational level.
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    Curative effect of Jiawei Yuyin Tang on threatened abortion after assisted reproductive technology
    YANG Li-li, HAN Yan-hua, HAN Ya-guang, AN Xin
    2014, 37 (9):  638-640.  doi: 10.3969/j.issn.1006-2157.2014.09.015
    Abstract ( 444 )   Save
    Objective To observe the curative effect of Jiawei Yuyin Tang on threatened abortion after assisted reproductive technology (ART). Methods The patients with threatened abortion after ART (n=70) and abortion history were chosen from Jan. 2009 to Jan. 2012, and then randomly divided into 2 groups (each n=35). The treatment group was treated with Jiawei Yuyin Tang and control group was treated with human chorionic gonadotropin (hCG). The changes of symptoms, levels of serum progesterone (P) and HCG and pregnancy rate were observed. Results The number of days of relieving symptoms were significantly less in treatment group than that in control group (P<0.05). The pregnancy rate was 88.6% in treatment group and 80.0% in control group (P<0.05). The levels of serum P and HCG had significant difference before and after treatment, while the difference between 2 groups had no statistical significance. Conclusion Jiawei Yuyin Tang is safe and effective in treatment of threatened abortion after ART, and can effectively relief symptoms. The mechanism may be related to that Jiawei Yuyin Tang can improve body’s immune function and endometrial receptivity.
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    Influence of therapy of tonifying spleen and replenishing kidney on level of serum leptin in patients with maintainance hemodialysis
    LI Zheng-sheng, SUN Bo, LU Jing-jing, WANG Ze-lan, XIE Juan, HUANG Bo, WANG Ye, CHENG Xiao-juan
    2014, 37 (9):  641-643.  doi: 10.3969/j.issn.1006-2157.2014.09.016
    Abstract ( 440 )   Save
    Objective To investigate the influence of therapy of tonifying spleen and replenishing kidney on level of serum leptin (LP) in patients with maintainance hemodialysis (MHD), and discuss the mechanism of the therapy in treating malnutrition of MHD patients. Methods The patients (n=60, male 25 and female 35) were randomly divided into treatment group and control group, and other health persons (n=30) were chosen as normal group. The treatment group was treated with therapy of tonifying spleen and replenishing kidney, and other 2 groups were given placebo for 6 m. The level of serum LP was compared in 3 groups before and after treatment. The changes of LP, hemoglobin (Hb), serum albumin and cholesterol (CH) were observed before and after treatment. Results The level of LP was significantly lower in normal group than that in other 2 groups before and after treatment (P<0.05), and decreased significantly in treatment group after treatment (P<0.05). The scores of SGA, symptom scores, and levels of serum albumin and CH were improved in treatment group after treatment (P<0.05). Conclusion The therapy of tonifying spleen and replenishing kidney can effectively reduce the level of LP and improve nutritional status in patients with MHD.
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    Influence of electroacupuncture-assisted intervention on ovarian hyperstimulation syndrome during in vitro fertilization-embryo transplantation
    HONG Yan-li, TAN Yong, YIN Yan-yun, ZOU Yi-jie, GUO Yin-hua, NIE Xiao-wei
    2014, 37 (9):  644-648.  doi: 10.3969/j.issn.1006-2157.2014.09.017
    Abstract ( 488 )   Save
    Objective To observe the influences of electroacupuncture-assisted intervention on incidence of ovarian hyperstimulation syndrome (OHSS) and pregnancy outcome during in vitro fertilization-embryo transplantation (IVF-ET). Methods The patients were randomly divided into observation group and control group. The observation group was treated with electroacupuncture from the day of injecting gonadotrophin (Gn) to the day of embryo transplantation (ET). The levels of serum estradiol (E2), vascular endothelial growth factor (VEGF), interleukin-6 (IL-6) and angiotensin-II (AT-II) were detected at the day of hCG injection, ovum pick-up (OPU) day and ET day. The incidence of OHSS and clinical outcomes were compared between 2 groups. Results The level of E2 was significantly lower in observation group than that in control group at OPU day and ET day (P<0.05). The levels of VEGF and IL-6 were significantly lower in observation group than those in control group at hCG day, OPU day and ET day (P<0.05). The difference in level of AT-II had no statistical significance between 2 groups (P>0.05). The incidence of OHSS was significantly lower in observation group than that in control group (P<0.05), and high-quality embryo rate and clinical pregnancy rate had no significant difference between 2 groups (P>0.05). Conclusion Electroacupuncture can reduce significantly the incidence of OHSS but will not reduce high-quality embryo rate and clinical pregnancy rate during IVF-ET, which may be related to the decrease of abnormally elevated vascular permeability.
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