Quality representation and correlation analysis of Fructus Perillae based on characteristic spectrum of medicinal system*
2015, 38 (4):
Objective To establish the quality representation and correlation analysis method based on the characteristic spectrum of medicinal system of Fructus Perillae (Zisuzi), so as to evaluate quality of Zisuzi pieces effectively and accurately. Methods Using established HPLC-PDA method to characterize the quality of Zisuzi characteristic spectrum of medicinal system. The quality of 15 batches of Zisuzi pieces was represented according to the number and chemical type of characteristic peaks on characteristic spectrum, and the quantity of 15 batches of Zisuzi pieces was represented based on the content of three characteristic index components of luteolin, apigenin,rosemary acid, flavonoids (represented by luteolin) and phenolic acids (represented by rosemary acid). Correlation analysis of quality and quantity representations was then performed referring to the benchmark pieces. Results As the reference substance, characteristic spectrum of Lot.15 contains 12 characteristic peaks, 5 peaks of flavonoids and 7 peaks of phenolic acids, which all appeared on the chromatograms of 15 batches Zisuzi pieces.Lot.15,6,9,10,2,5,7 Zisuzi pieces had more effective index components. After comprehensive evaluation of more total effective index components of Lot.6, 12, 11, 7, 4, 14 ,10 and high correlation degree of Lot. 6, 12, 11, 7, 4, 14, 10 correlated with Lot. 15, the quality of Lot. 15, 6, 7, 10, 2, 5, 9, 12, 11,14 were judged to be better. Conclusion The established HPLC method by quality and quantity representation of characteristic spectrum of 15 batches of Zisuzi pieces is simple and accurate. And the evaluation mode of quantity and quality and their correlation based on Zisuzi medicinal system, concerning on systemic correlation and application validity, can evaluate the quality of Zisuzi pieces effectively and accurately.
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