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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    15 June 2015, Volume 38 Issue 6 Previous Issue    Next Issue
    Ganzhi Yunqi and constitution: correlation and philosophical interpretation*
    HE Juan
    2015, 38 (6):  365-368.  doi: 10.3969/j.issn.1006-2157.2015.06.001
    Abstract ( 547 )   PDF (1170KB) ( 954 )   Save
    The theory of five circuits (yun) and six climatic factors (qi), an indispensable part of Traditional Chinese Medicine, studies the cycle of climatic changes and its influence on life activities. According to the theory of Huangdi’s Internal classic, The influence of ganzhi (heavenly stems and earthly branches) yunqi on life activity is not limited to the development of diseases or syndromes related to changes of yunqi within corresponding year; this impact also extends to inherent physical constitution determined by the yunqi of pregnancy year. This physical characteristic is also associated with its susceptibility to diseases. Yunqi theory originates from the theory of qi transformation in Huangdi’s Internal classic, which is the extension of qi-monism theory in ancient philosophy.
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    Tongue coating, disease and pattern*
    ZHANG Xin-yang, GUO Zi-ning
    2015, 38 (6):  373-376.  doi: 10.3969/j.issn.1006-2157.2015.06.003
    Abstract ( 482 )   PDF (1080KB) ( 619 )   Save
    Tongue coating is an essential component in the diagnostic system of Traditional Chinese Medicine. Modern research has demonstrated that tongue coating can be indicative of diseases, especially digestive diseases. In our clinical practice, we also observed such phenomona and reviewed the pathological basis for some of the tongue coating manifestations. Corresponding treatment methods were offered to test the relations between tongue coating, diseases, and patterns, so as to propose a hypothesis that tongue coating can be used as an early sign of diseases. This paper explores the relation between tongue coating, diseases, and patterns on the basis of current research on tongue coating mechanism. The results of the paper could offer reference for the clinical application of tongue coating diagnosis and facilitate the standardization of objective tongue diagnosis.
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    Effects of heat-clearing and blood-activating combination on acute cerebral ischemia of heat toxin pattern in rats via NF-κB signaling pathway*
    LIANG Xiao, ZHANG Yun-ling, WANG Xin-xiang, LIU Xue-mei, ZHENG Hong, YAO Ting, WANG Jian-wei
    2015, 38 (6):  377-382.  doi: 10.3969/j.issn.1006-2157.2015.06.004
    Abstract ( 444 )   PDF (1129KB) ( 627 )   Save
    Objectives To study the synergetic effects of heat-clearing and blood-activating combination in the treatment of acute cerebral ischemia of heat toxin pattern. Methods Animal model of acute cerebral ischemia of heat toxin pattern was established by using middle cerebral artery occlusion (MCAO) and carrageenan injection in rats. Rats were randomly divided into eight groups: normal group, sham group, ischemia 1.5 h/reperfusion 72 h group, Kudiezi(KDZ) injection group (heat-clearing, group A), Xueshuantong(XST) injection group (blood activating, group B), KDZ1.8 mL/kg+XST20 mg/kg (group C), KDZ1.8 mL/kg+XST80 mg/kg (group D), and KDZ7.2 mL/kg+XST20 mg/kg (group E). Infarction area ratios was measured by TTC staining; activity or content of NF-κB p65 and IKKβ of cerebral tissues, by Western bolt; gene expression of NF-κB p65 and IKKβ, by real- time PCR. Results Cerebral tissue ischemia in all treatment groups was reduced (P<0.05). Heat-clearing and blood-activating combined treatment improved significantly (P<0.01) compared with the model group, yet there were no significant differences between these groups. NF-κB p65 and IKKβ in model group were highly expressed (P<0.01) compared with the normal group. They both decreased in all treatment groups. NF-κB p65 protein expression in group C and E significantly decreased compared with the model group (P<0.01); and they also decreased (P<0.05) in group A and B. Expression of IKKβ protein decreased in combination treatment groups (group C, D, E and A) markedly (P<0.01). In addition, IKKβ protein expression in groups C, D and E were lower than that in group B significantly (P<0.01); It was markedly lower in groups C and E than in KDZ group (P<0.01). Expression of NF-κBp65、IKKβ mRNA in the model group was higher (0.48%) than that in the normal group (0.37%). NF-κB p65 and IKKβ mRNA expression in each treatment group decreased compared with the model group. Expression of NF-κB p65 and IKKβ mRNA in group D and the expression of IKKβ mRNA in group E was lower than the model group; the expression of NF-κB p65 mRNA in group E was significantly different compared with the model group. Conclusion Heat-clearing and blood-activating combination has synergetic effects on acute cerebral ischemia via regulating the protein and gene expression of NF-κB p65 and IKKβ in NF-κB signaling pathway.
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    Effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of cerebral bax and bcl-2 in rats with generalized anxiety disease*
    DONG Ning, TANG Qi-sheng, ZHAO Rui-zhen, LUO Bin, WANG Wen-long
    2015, 38 (6):  383-387.  doi: 10.3969/j.issn.1006-2157.2015.06.005
    Abstract ( 450 )   PDF (1129KB) ( 583 )   Save
    Objective To explore the effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of bax and bcl-2 in cerebral Papez’s circuit in rat with generalized Anxiety Disease (GAD). Methods A total of 40 male Wistar rats were divided into normal group, model group, treatment group, and control group, with 10 rats in each group. GAD model was established by using the stimulation of uncertain empty drinking bottle. Then the treatment group and control group were intragastrically given Danzhi Xiaoyao San (Cortex Moutan and Fructus Gardeniae Free Wanderer Powder) and Buspirone Hydrochloride once a day for 7 consecutive days respectively. The model group was given distilled water once a day for a week. The expressions of bax and bcl-2 in rat hippocampus and mamillary body were measured by applying western blot assay after resecting rat brains. Results The expression of bax in hippocampus and mamillary body of model group increased significantly compared with normal group (P<0.05 or P<0.01) and the expression of bcl-2 decreased markedly (P<0.01). Compared with model group, the level of bax in hippocampus and mamillary body of treatment group and control group was reduced (P<0.05 or P<0.01) and the level of bcl-2 increased significantly (P<0.05 or P<0.01). Conclusion The therapeutic method of liver-soothing heat-clearing and spleen-fortifying method represented by Danzhi Xiaoyao San could reduce the level of bax in hippocampus and mamillary body of GAD rat brain, elevate the level of bcl-2 and reduce bax/bcl-2, prevent and inhibit cell apoptosis in Papez’s circuit and then ameliorate anxiety symptoms.
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    Comparing truncated and routine methods in the treatment of mice pneumonia induced by influenza virus*
    DONG Ying-ying, WU Jun, CHEN Dan-jun, LI Gen-mao, GE Dong-yu, QIU Ze-ji, HAO Yu
    2015, 38 (6):  388-392.  doi: 10.3969/j.issn.1006-2157.2015.06.006
    Abstract ( 416 )   PDF (1039KB) ( 424 )   Save
    Objective To compare the effects of truncated and routine methods in the treatment of mice pneumonia induced by influenza virus. Methods ICR mice (n=80) were randomly divided into normal group, model group, routine treatment group and truncated treatment group. The last three groups were infected with mouse lung-adapted influenza virus strain by inoculating intranasally. One hour after the inoculation, the truncated treatment group was intragastrically given Xijiao dihuang Tang (Rhinoceros Horn and Rehmannia Decoction) combined with Yinqiao San (Lonicera and Forsythia Powder) decoction while the routine treatment group was intragastrically given Yinqiao San decoction only on the first three days and Xijiao dihuang Tang only on the following four days. All groups were treated for 7 consecutive days. The survival rate, average survival days and body weights were calculated during 14 days’ observation. Balb/c mice (n=128) were divided into the same four groups described above with the same corresponding intervention. These mice were sacrificed on the 2nd, 4th, 6th and 8th days respectively. Specimen were obtained and evaluated. The viral titers in lung homogenate and the lung index were measured dynamically; the lung gross lesions and tissue pathological changes were observed on the 8th day. Results The survival rate of truncated treatment group was twice as high as routine treatment group and average survival days also increased significantly; the lung index of truncated therapy group significantly decreased compared with the routine group on the 8th day. The viral titers of truncated treatment group in lung homogenate decreased on the 2nd, 4th, 6th and 8th day compared with the routine treatment group, yet the difference was not statistically significant. The pulmonary gross lesions and tissue pathological changes under light microscope were less severe than the routine treatment group. Conclusion Truncated method seemed to be superior to routine method in the treatment of severe influenza viral pneumonia mice. Its mechanisms may not lie in its stronger anti-viral effect, but in its stronger inhibitory effect on inflammatory cascade after viral infection compared with routine treatment.
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    Quality representation and correlation analysis of Puhuang based on quality evaluation model of drug system*
    LI Gang, XU Shu-ya, ZHAO Li-min, LU Guang-ying, SUN Dao-han, WANG Ya-li, Tang Xue-yang, PENG Ping, JIANG Yan-yan, SHI Ren-bing
    2015, 38 (6):  393-399.  doi: 10.3969/j.issn.1006-2157.2015.06.007
    Abstract ( 464 )   PDF (1226KB) ( 370 )   Save
    Objective To establish quality representation and correlation analysis method of Puhuang (Pollen Typhae Angustifoliae) pieces based on drug system and evaluate Puhuang quality comprehensively. Methods HPLC was used for simultaneous determination of five major active ingredients and their total amount in 16 batches of Puhuang (Pollen Typhae Angustifoliae.) decocting pieces, including typhaneoside, isorhamnetin-3-O-neohesperidoside, isorhamnetin, stigmasterol, and β-sitosterol. Ferric chloride-potassium ferricyanide colorimetric method was used for measuring the content of phenols in flavonoids while vanillin-perchloric acid colorimetric method was used for the determination of pollen by measuring the total sterol content of pollen Typhae. Non-correlation coefficient, non-correlation degree, and correlation degree were used to represent quality correlation between samples and reference products. Results Levels of active ingredients were higher in No.7, 3, 5, 4, 10 and 13 Puhuang pieces than No.8 (reference product). No.5, 7, 6, 10, 1, and 13 batches had high relevance with the reference product No.8. Consolidated quality characterization and correlation analysis showed that the quality of No. 7, 5, 10, and 13 were superior. Conclusion Based on drug quality evaluation system, measurement of active ingredient levels, total amount and relative ratio combined with correlation analysis of reference product with definite efficacy could offer a comprehensive assessment of quality of Puhuang decocting pieces. This method could provide evidence for screening, quality control and application of Puhuang decocting pieces. It also serves as references for quality evaluation of Chinese materia medica both in methodology and application.
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    Analyzing chemical composition of Mongolian compound Sengdeng-4 in vivo and in vitro*
    SA Chu-la, BAI Pu, DONG Yu, XING Kai-xia
    2015, 38 (6):  400-404.  doi: 10.3969/j.issn.1006-2157.2015.06.008
    Abstract ( 482 )   PDF (1934KB) ( 731 )   Save
    Objective To establish the characteristic chromatograms of Mongolian compound Sengdeng-4 in vivo and in vitro, analyze and identify its chemical components in vitro and serum. Methods After the model of rheumatoid arthritis was established, SD rats were orally administered Mongolian compound Sengdeng-4 and each single ingredient respectively. The medicinal serum were separated and measured with HPLC. The HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 in vitro and medicinal serum were identified. The characteristic chromatograms of Mongolian compound Sengdeng-4, single ingredient in vitro and medicinal serum were analyzed, and the chemical composition of Mongolian compound Sengdeng-4 in vitro, original crude medicinal in blood were identified and compared. Results The results of HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 showed that 47 peaks were identified. There were 14 chemical compositions absorbed into blood, among them 9 were original ingredients and 5 were metabolites. The second peak was from Wenguanmu; the fifth, sixth, and seventh peaks were from Zhizi; the first, eighth, and tenth peaks were from Hezi. The third peak was from Wenguanmu, Chuanlianzi and Hezi. The fourth and thirteenth peaks were from Mongolian compound Sengdeng-4. The ninth peak was from Wenguanmu and Chuanlianzi. The eleventh, twelfth and fourteenth peaks were from Wenguanmu and Hezi. 7 components of Mongolian compound Sengdeng-4 were identified. Conclusion The chemical compositions and metabolites in blood may be the active ingredients with direct actions in vivo, which come from the ingredients of Mongolian compound Sengdeng-4.
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    Spectrum-effect relation in antioxidant activity of Menthae haplocalycis Herba based on DPPH and FRAP assay*
    XU Jing-jing, LIU Bin
    2015, 38 (6):  405-410.  doi: 10.3969/j.issn.1006-2157.2015.06.009
    Abstract ( 502 )   PDF (1525KB) ( 605 )   Save
    Objective To explore the active ingredients of Menthae Haplocalycis Herba by investigating the correlation between HPLC spectrum and antioxidant activity. Methods We established DPPH[1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH] and FRAP(ferric ion reducing antioxidant power, FRAP) assay to determine the antioxidant activity of Menthae Haplocalycis Herba. The spectrum-effect correlation was studied by using PLS(Partial least squares). HPLC-MS/MSn was applied to identify their structures. Results Six compounds including hesperidin, diosmin, buddleoside, thymusin and xanthomicrol, which made great contributions to the DPPH scavenging activity, were screened; eight compounds, including hesperidin, buddleoside, rosmarinic acid and trans Salvianolic acid J, were obtained which made great contributions to the total antioxidant activity. Conclusion It is an effective way to characterize spectrum-efficacy correlation and clarify the active components of Chinese medicinal.
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    Using standard chinese medicine decation pieces as reference substance: Issues for discussion*
    LI Li, LIU Ying,XIAO Yong-qing
    2015, 38 (6):  411-412.  doi: 10.3969/j.issn.1006-2157.2015.06.010
    Abstract ( 594 )   PDF (987KB) ( 703 )   Save
    The standard system of quality evaluation on Traditional Chinese Medicine is composed of standard Chinese medicine decoction pieces, chemical reference substance, control Chinese herbs and control extracts. This paper is aimed to discuss the significance, fields and methods on standard Chinese medicine decoction pieces (standard pieces) applied as standard materials from the aspects of preparation, recognition of property and identification of characteristic, by which the unique advantages of standard pieces as the standard materials is explained, leading to beneficial exploration on further improvment of the standard system of quality evaluation on Traditional Chinese Medicine.
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    A survey of Chinese public knowledge about Traditional Chinese Medicine*
    XU Si-qun, JIN Qi, WANG Hui, LI Zi, JIANG Xiang, LYU Mei, TAN Wei
    2015, 38 (6):  413-419.  doi: 10.3969/j.issn.1006-2157.2015.06.011
    Abstract ( 458 )   PDF (1060KB) ( 458 )   Save
    Objective To understand Chinese public knowledge of Traditional Chinese Medicine (TCM) and offer references for general education approaches. Methods Based on government guideline and previous research, a questionnaire was designed and distributed randomly among Chinese residents in the country. Among the 1,800 questionnaires distributed, 1,486 was returned with 1,050 valid ones identified. The questionnaire consisted of seven demographic items including gender and ages; it also included seventeen items of general TCM knowledge such as the nature of TCM discipline, advantages of TCM, and common sense of health care in TCM. SPSS 20.0 software was used to analyze the data obtained. Mean and standard deviation (SD) was calculated for normal distribution and the homogeneity of variance test. One-way ANOVA analysis was used to compare differences between groups. LSD test was conducted for homogeneous variance and Tamhance’T2 test for heterogeneous results. Results Chinese public knowledge about TCM was overall limited. Average score ()of all 17 questions was 2.07 (SD=0.53). The best known item was precautions for decoction administration(=2.47)while the worst known one was about theory of five phases(=1.78). Comparison among groups indicated that students knew the most about TCM when compared with peasants(P<0.001,Δaverage=6.125); Difference was the most significant between groups of different incomes (5 000-999 RMB/month vs. 1 000-4 999 RMB/month, P<0.001,Δaverage=2.870). Health status over the past year influenced the public’s knowledge as there was a significant difference between “I have been feeling better” and “I have been feeling worse” groups (P<0.001,Δaverage=7.058) Conclusions Chinese public knowledge about TCM was generally limited. General education is in urgent need to provide systematic training to help the public know more about TCM philosophy and basic theories. Educators need to work from the grass to promote public’s health care and benefit social awareness. The quantitative assessment of TCM general education also merits improvement. This study proves to be a positive innovation.
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    Dahuang Zhechong Wan for chronic hepatitis B: a systematic review*
    DENG Yan-fang, QU Le, SONG Ya-nan, JIANG Yan
    2015, 38 (6):  420-425.  doi: 10.3969/j.issn.1006-2157.2015.06.012
    Abstract ( 424 )   PDF (2276KB) ( 445 )   Save
    Objective To evaluate the effectiveness of Dahuang Zhechong Wan (Rhubarb and Eupolyphaga Pill) for Chronic Hepatitis B. Methods Randomized controlled trials (RCTs) of Dahuang Zhechong Wan for Chronic Hepatitis B published in domestic or foreign magazines were searched. Revman5.2 was used to perform the Meta-analysis on the curative effect of Dahuang Zhechong Wan for chronic hepatitis B. Results A total of 21 RCTs were included, among which none used Dahuang Zhechong Wan only.21 studies all used combined treatment on 1731 cases with 875 in treatment groups and 856 in control groups. The results of meta-analyses showed that at the end of the treatment, groups adding Dahuang Zhechong Wan was superior to those without. There were significant difference in both the clinical efficiency, HBV-DNA negative conversion, HBeAg negative conversion, ALT and TBil recovery. Conclusion Combined therapy using Dahuang Zhechong Wan is superior to conventional medicine alone for chronic hepatitis B. However, because of low quality of the included studies, high-quality and large-scale RCTs are needed to provide further evidence.
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    Effects of acupuncture with different stimuli intensity on uterine contraction and expression of endocrine-related molecular receptor genes in rats with dysmenorrhea of cold coagulation pattern*
    HSU Wing-sze, ZHAO Guo-zhen, JI Bo, QI Dan-dan, GUO Meng-wei, REN Xiao-xuan, ZHAO Ya-fang, ZHANG Lu-fen, LI Xiao-hong, ZHU Jiang, ZHANG He, SONG Yan, LIU Ya-li, ZHANG Ping
    2015, 38 (6):  426-429.  doi: 10.3969/j.issn.1006-2157.2015.06.013
    Abstract ( 411 )   PDF (1032KB) ( 468 )   Save
    Objective To evaluate the effects of acupuncture with different stimulation intensity at Sanyinjiao (SP6) on uterine contraction and expression of endocrine-related molecular receptor gene in rats with dysmenorrhea of cold coagulation pattern and to explore whether there is association between “deqi (qi arrival)” and stimulus intensity. Methods Diestrus female rats were randomly divided into 4 groups, including saline control group, model control group, deqi group (with strong stimulus) and No deqi group (with weak stimulus). The levels of uterine contraction was recorded during 20 mins acupuncture treatment. The expression of PGF2α mRNA was measured. Results Compared with the model control group, the uterine contraction wave number and uterine activity were significantly reduced in deqi group. The expressions of PGF mRNA in both deqi and No deqi group were also reduced compared with model group. Conclusion Our finding indicates that using thick needles, deep insertion with hand-manipulation seems more effective and could achieve desired level of “deqi” in dysmenorrhea rats.
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