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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 September 2015, Volume 38 Issue 9 Previous Issue    Next Issue
    Discussion and clinical verification for collateral vessels, diseases collateral and collateral diseases*
    WANG Xian, WANG Yongyan
    2015, 38 (9):  581-586.  doi: 10.3969/j.issn.1006-2157.2015.09.001
    Abstract ( 511 )   PDF (1215KB) ( 615 )   Save
    It is well known that the collateral vessels, in TCM, is the unity of structures and functions of qi collaterals, blood collaterals and collateral loops. The term of “collateral vasculopathy” refers to the process of the normal collateral vessels turning into the diseased collateral vessels and leading to the collateral vessel diseases at last. And the syndrome of stirring wind arising from “collateral vasculopathy” is defined as “endogenous collateral-wind”, the presentation form of diseased collateral vessels, reflecting the mutability of shapes and characters of collateral vessels. The syndrome of “endogenous collateral-wind” is due to collateral deficiency, heat-toxin stagnation or exogenous wind. Furthermore, the concepts of “endogenous collateral-wind” and “endogenous liver-wind” have something in common, but not really the same. The theory of endogenous collateral-wind have been successfully applied to percutaneous coronary intervention (PCI) post-operation, acute exacerbation of heart failure or cardiorenal syndrome, and tachyarrhythmias. It will become one of directions of TCM scientific research, we believe, that the study for the theory of endogenous collateral-wind to treat collateral vessel diseases guiding the treatment and diagnosis of multiple difficult and complicated diseases.
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    Automatic identification of TCM terminology in Shanghan Lun based on conditional random field*
    MENG Hongyu ,XIE Qingyu ,CHANG Hong ,MENG Qinggang
    2015, 38 (9):  587-590.  doi: 10.3969/j.issn.1006-2157.2015.09.006
    Abstract ( 485 )   PDF (1226KB) ( 766 )   Save
    Objective To explore the methods of automatic identification of TCM terminology and to expand the forms of natural language processing in TCM documents. Methods Based on the methods of conditional random field(CRF), annotation and automatic identification on terms of symptoms, diseases, pulse-types and prescriptions recorded in Shanghan Lun as the research subjects, the effects of different combinations of the features, such as Chinese character itself, part of speech, word boundary and term category label, on identification of terminology were analyzed and the most effective combination was selected. Results The TCM terminology automatic identification model, combining with the features of Chinese character itself, part of speech, word boundary and term category label, had the precision of 85.00%, recall of 68.00% and F score of 75.56%. Conclusion The multi-features model of combination of Chinese character itself, part of speech, word boundary and the term category label achieved the best identifying result in all combinations.
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    Effects of Chaiqi decoction on glucose and lipid metabolism and insulin resistance in rat model with metabolic syndrome*
    WANG Ying, LIU Jing,WANG Xinxiang, ZHANG Liping
    2015, 38 (9):  597-600.  doi: 10.3969/j.issn.1006-2157.2015.09.004
    Abstract ( 407 )   PDF (1058KB) ( 395 )   Save
    Objective To study the effects of Chaiqi decoction (CQD)at different doses on glucose and lipid metabolism and insulin resistance(IR) in metabolic syndrome (MS)rat model. Methods The MS rat model was induced by feeding diet of high fat, high sugar and high salt. Altogether 56 male rats were randomly divided into normal group(n=16), model group(n=16), CQD low dose group(low-dose group, n=8), CQD middle dose group(mid-dose group, n=8) and CQD high dose group(high-dose group, n=8). The low-, mid-, and high- groups were taken CQD by intragastric administration at dose of 1.90, 5.71 and 17.13 g/kg daily for four weeks. At the end of the 8th week, eight rats of normal group and model group were sacrificed, and at the end of the 12th week, all the rats in each group were sacrificed. Serum TG, TC, HDL-C, LDL-C, fasting blood glucose (FBG) and fasting insulin (FINS) were detected, while insulin resistance index (IRI) was calculated. Results After taking CQD for four weeks , compared with model group, FINS and TG in low-dose group decreased significantly (P<0.05) while HDL-C increased significantly (P<0.05); FINS, TG, TC, IRI decreased significantly while HDL-C increased significantly in both mid- and high- dose groups (P<0.05). Conclusion CQD would attenuate the lipid metabolism of rats with metabolic syndrome and reduce IR. Furthermore, the mid-dose group and high-dose group got better effects than those of low-dose group.
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    Effect of the method of clearing heat and resolving phlegm on the airway mucus hypersecretion of the AECOPD rat model with phlegm-heat syndrome*
    LI Jie, WANG Qi, ZHANG Lishan, QIU Zeji,WU Jun, WANG Linyang
    2015, 38 (9):  601-605.  doi: 10.3969/j.issn.1006-2157.2015.09.005
    Abstract ( 420 )   PDF (1129KB) ( 450 )   Save
    Objective To study the effect of Louqin Zhisou decoction (LQZSD), a Chinese herbal medicine compound formulated by the TCM method of clearing heat and resolving phlegm, on the airway mucus hypersecretion of the AECOPD rat model, so as to improve the transportation of antibiotics in lung tissue. Methods Altogether 50 Wistar male rats were randomly divided into normal group , model group , ambroxol group , Erchen decoction (ECD) group, Louqin Zhishou decoction(LQZSD) group (each n=10). The rat model of AECOPD with phlegm-heat syndrome was established by passive cigarette smoking,intratracheal instillation of lipopolysacchricle (LPY) and intranose instillation of staphylococcus aureus combined with wind-heat stimulation. After modeling, ambroxol group was intraperitoneally injected ambroxol at dose of 6 mg/kg per day, ECD group and LQZSD group were intragastrically administered ECD and LQZSD at dose of 3.33 g/kg per day respectively. The treatment duration was seven days. The secretion of acidic mucin in goblet cells was observed by using AB-PAS staining. The goblet cells in each visual field were counted and the proportion of goblet cells to epithelial cells was calculated. The contents of serum neutrophil elastase (NE)and MUC5AC in bronchoalveolar lavage fluid (BALF)were detected by using ELISA assay. Results Compared with model group, the secretion of acidic mucin and the number of goblet cells significantly decreased in each treatment group(P<0.05). Furthermore, serum NE and MUC5AC both decreased in LQZSD group significantly(P<0.05), while there were no statistical differences in the other treatment groups. Conclusion The potential mechanism of LQZSD improving the transportation of antibiotics would be related to reduction the secretion of acidic mucin and the number of goblet cells, as well as decrease the contents of serum NE and MUC5AC in BALF; as the result, the reduction of the secretion of mucus in airway led to clearance the airway obstruction and promotion the forward diffusion of antibiotics in rat’s lung tissue.
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    Effects of serum containing modified Shengmai liquid on migration and adhesion of non small cell lung cancer cells*
    CHEN Hui, JIANG Miao, XIN Lili,QU Jinglian, XU Muge,SU Yinxu,GONG Jiening
    2015, 38 (9):  606-610.  doi: 10.3969/j.issn.1006-2157.2015.09.008
    Abstract ( 428 )   PDF (1070KB) ( 382 )   Save
    Objective To study the effects and mechanism of serum containing modified Shengmai liquid (MSL)on migration and adhesion of non small cell lung cancer (NSCLC) cells. Methods Rabbit serum containing MSL was prepared by intragastric administration (i.g.)with MSL at the dose of 18 g/kg and control serum was prepared by i.g. normal saline. NSCLC cells were cultured, and those in logarithmic growth phase collected were divided into four groups: 10% control serum(CS) group, 2.5%, 5%, 10% MSL serum group. Migration cells were detected by using Transwell invasion assay, and adhension rate was measured by using matrigel adhensive assay. The expressions of N-cad and vimentin were determined by RT-PCR. Results The number of migration cells decreased as the concentration of serum containg MSL increased:migration rate of 5% MSL group was 25.64% and that of 10% MSL group was 13.31%(P<0.01). As the concentration of serum containing MSL increased and the duration of treatment prolonged, heterogeneous adhesion rate decreased and was negatively correlated with serum concentration and duration; while the gene expressions of N-cad and vimentin decreased in dose- and time- dependent manner. Conclusion The mechanism of inhabitation migration and reduction heterogeneity adhension of MSL on NSCLC cells would be related to the epithelial-mesenchymal transition.
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    Effects of TCM compound of resolving stagnation for tranquilization on neurotransmitters and brain-derived neurotrophic factors in depression rat model by chronic unpredictable stress*
    ZOU Haiyan, YANG Yilong, TU Hengjing, YAO Xiaoquan, WANG Yali, LIU Sisi, OUYANG Junyao, WANG Haizheng, ZHAO Hui, WANG Lei, ZHANG Chi, ZHANG Qiuxia
    2015, 38 (9):  611-618.  doi: 10.3969/j.issn.1006-2157.2015.09.007
    Abstract ( 416 )   PDF (1098KB) ( 358 )   Save
    Objective To observe the effect of TCM compound of resolving stagnation for tranquilization (RST) on neurotransmitters and brain derived neurotrophic factors (BNDF) in depression rat model induced by chronic unpredictable stress (CUS), and to reveal the mechanism of RST on the treatment of depression. Methods Altogether 60 male SD rats were randomly divided into normal group, model group, high-, mid- and low- dose RST groups at dosage of 8.2, 16.3, 32.7 g/kg, and fluoxetine group with dose of 10 mg/kg. The rats of four therapy groups were administered intragastrically drugs with undergoing CUS for consecutive thirty-five days. The behavioral changes of rats were observed by open field test and novelty-suppressed feeding-test. The content of dopamine (DA), 5-hydroxeytryptamine(5-HT), 5-hydroxyindole acetic acid (5-HIAA) and norepi-nephrine(NE) in hippocampus and cortex were determined by HPLC. And the expression of BDNF in hippocampus was measured by Western blot. Results Compared with the normal group, the rats’ weight increased slowlier and the spontaneous activity decreased in model group; the contents of 5-HT and NE in cortex and the content of 5-HT, 5-HIAA and NE in hippocampus significantly reduced (P<0.05). Compared with the model group, the spontaneous activity significantly increased in all therapy groups; the contents of 5-HT and NE in cortex dramatically increased in fluoxetine group and mid-, high- dose group (P<0.05); the contents of 5-HT and 5-HIAA in hippocampus significantly increased in all three RST groups (P<0.05); the content of NE in hippocampus significantly increased in low- and high- dose group (P<0.05). The expression of BDNF in hippocampus significantly increased in fluoxetine group and high-dose RST group (P<0.05). Conclusion The anti-depression mechanism of TCM compound formulated by principle of resolving stagnation for tranquilization may be related to the effects of increasing neurotransmitters and up-regulation of BDNF.
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    Gene and protein expression of B cell-activating factor and its receptor in MRL/lpr lupus mice with different constitutions*
    WANG Dongjian, SU Li,WANG Xiao, QU Huanru,MAO Jianchun,GU Junhua,DING Zhijiang, ZHOU Jun
    2015, 38 (9):  619-623.  doi: 10.3969/j.issn.1006-2157.2015.09.009
    Abstract ( 362 )   PDF (1169KB) ( 467 )   Save
    Objective To establish the method to distinguish the constitution of MRL/lpr lupus mice and to study the gene and proteinexpression of renal B cell-activating factor (BAFF), B cell stimulating factor receptor (BAFF-R), so as to reveal the factors at molecular biological level leading to the differences of constitution. Methods Firstly, MRL/lpr female mice of 12-week age were classified into cold constitution, heat constitution and normal constitution. Secondly, 20 mice were randomly selected respectively from cold constitution group, heat constitution group and normal constitution group. Thirdly, every 10 mice in each group were sacrificed at 12-week age and at 20-week age respectively. Then, expressions of renal BAFF mRNA, BAFF-R mRNA, BAFF protein and BAFF-R protein were examined by real-time PCR and Western Blot. Results Expressions of BAFF mRNA ,BAFF protein and BAFF-R protein were the lowest in the cold constitution group of all the groups. The expression of BAFF-R mRNA showed no significant difference among all groups. Conclusion The constitutional differences could be related to the expressions of renal BAFF mRNA ,BAFF protein and BAFF-R protein .
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    Characteristic of intestinal absorption of euphorbia factor L1 by rats single pass intestinal perfusion moder in situ*
    ZHANG Xiuting, WANG Yingzi, LI Shaojing,DUAN Feipeng, WANG Qing, ZHANG Chunni, LI Fengying,LI Wenhua, LUO Shengxiu
    2015, 38 (9):  624-628.  doi: 10.3969/j.issn.1006-2157.2015.09.010
    Abstract ( 358 )   PDF (1155KB) ( 373 )   Save
    Objective To study the characteristic of absorption of euphorbia factor L1 in intestine of rats, and to observe the effects of P-glycoprotein(P-gp)and multidrug resistance-associated protein(MRP2)on intestinal absorption of euphorbia factor L1. Methods The contents of euphorbia factor L1 of intestinal perfusion fluid of duodenum, jejunum, ileum and colon in the rats in situ single-pass intestinal perfusion model were determined by using HPLC. The drug absorption rate constant(Ka) and the apparent absorption coefficient(Papp) in four intestinal regions were calculated. Results Ka and Papp of euphorbia factor L1 at colon were the highest of the whole rat intestine. Significant increase of Ka and Papp showed at rat colon when co-perfused with verapamil hydrochloride, by contrast, decrease of Ka and Papp found when co-perfused with indomethacin. Conclusion We inferred that verapamil hydrochloride be the substrate of P-gp and that indomethacin be not the substrate of MRP2.
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    Quality assessment of reporting in randomized controlled trials of Chinese herbal medicines for the treatment of Alzheimers disease using CONSORT criteria *
    NI Jingnian, WEI Mingqing, SHI Jing , ZHANG Xuekai, TIAN Jinzhou
    2015, 38 (9):  628-634.  doi: 10.3969/j.issn.1006-2157.2015.09.011
    Abstract ( 416 )   PDF (1312KB) ( 543 )   Save
    Objective To assess the quality of reports and abstracts of randomized controlled trials (RCT) of treating Alzheimer’s disease (AD) with Chinese herbal medicines. Methods The RCT reports were included from online search of database of PubMed and CNKI. Based on the Consolidating Standards for Reporting Trials (CONSORT) checklists, supplementary checklists of herbal medicine intervention and requirements of medical design, the quality of studies were performed. Results Eleven reports in English and eighteen ones in Chinese published in general medical journals were included. The number of items in six reports (20.7%)was over 50%, while the mean items in Chinese was 10.94 (30%)and in English 20.27(55%). Altogether nine reports (31%)got the Jadad score of three or above, while the mean Jadad score of reports in Chinese was 1.67±0.77 and in English was 2.61±0.93. Seven reports(24.1%)got the score of 2.5 or above in checklists of herbal medicine intervention, in which in Chinese got the mean score of 1.58±0.39 and English got 2.61±0.93 . Zheng (TCM syndrome) was reported only in one paper(3.4%)in Chinese, and comparison of the types between and after treatment was conducted. Conclusion The quality of reports in English was better than that in Chinese. The main problems in Chinese study reports existed in lacking of details of random design and blind design, insufficient treatment duration, improper positive drugs controlled and poorly targeted indices selected.
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    Comparison of long-term prognosis of chronic heart failure patients with different TCM syndrome elements in real world*
    XU Zheng, ZHANG Bingxuan, SONG Qingqiao, TIAN Yu, HUANG Wei
    2015, 38 (9):  635-640.  doi: 10.3969/j.issn.1006-2157.2015.09.012
    Abstract ( 424 )   PDF (1813KB) ( 594 )   Save
    Objective To compare the long-term prognosis of chronic heart failure (CHF) patients with different TCM syndrome elements of phlegm stagnation,qi deficiency, yin deficiency, blood statis and yang deficiency. Methods Subjects were 191 CHF inpatients in Guang’anmen Hospital from January 2006 to August 2011. The clinic information of the patients were collected, including general information, incidence and TCM syndrome elements. After discharging from the hospital, all the patients were visited in outpatient or followed up by telephone for years to get the occurrence and duration of all-cause mortality. Results The long-term prognosis of CHF patients with TCM syndromes ranked from the best to the worst: phlegm stagnation, qi deficiency, yin deficiency, blood stasis and yang deficiency (P<0.01). The prognosis of patients with syndrome of phlegm stagnation was better than those without phlegm stagnation (P=0.017); patients with syndrome of qi deficiency had a better prognosis than those without qi deficiency (P=0.011); there was no significant difference in patients with or without yin deficiency (P=0.633); the overall prognosis of blood stasis patients showed no significant difference with non blood-stasis patients (P=0.182), but went worse from the 500th day; the prognosis of patients with syndrome of yang deficiency was worse than patients without yang deficiency (P=0.002). Conclusion The long-term prognosis of CHF patients is definitely poor. The prognosis of CHF patients with different TCM syndromes showed striking difference. Syndrome of qi deficiency and syndrome of phlegm stagnation would be ordinary in early stage of CHF, and syndrome of yin deficiency played a role through the whole progression of CHF. The effect of syndrome of blood stasis could be associated with CHF disease progression, and the prognosis of patients with blood statis was worse. The syndrome of yang deficiency would be the main syndrome in late stage.
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    Effect of Yupao decoction on follicle blood flow and pregnancy rate in patients undergoing artificial insemination by husband *
    LI Nan , JIN Zhe , LIU Yanxia
    2015, 38 (9):  641-644.  doi: 10.3969/j.issn.1006-2157.2015.09.013
    Abstract ( 362 )   PDF (1056KB) ( 353 )   Save
    Objective To explore the effects of Yupao decoction (YPD) , a Chinese herbal compound formulated by the method of reinforcing kidney, on the follicle blood flow and pregnancy rate in patients undergoing artificial insemination by husband (AIH) . Methods Altogether 60 infertile women with small follicular ovulation undergoing AIH were divided into control group(n=34) and YPD group(n=26) according to random number table. Patients in control group were induced ovulation by clomiphene, and those of YPD group were taken ovulation induction with orally administration YPD. The pulsatility index (PI) and resistant index (RI) of follicle blood flow at the day of HCG injection and pregnancy rate (PR) were observed. Results PI , RI , PR were dramatically higher in YPD group than those of control group(P<0.05). In YPD group, the clinical PR was 34.61 % (9/26)and cycle PR was 19.56 %(9/46), by the contrast, the clinical PR was 11.76%(4/34) and the cycle PR 6.06%(4/66)in control group. Conclusion TCM kidney-reinforcing method combined with clomiphene could significantly improve the clinical pregnancy rate of patients undergoing AIH. The potential mechanism of combined therapy would exist in the improve ment the follicle quality by increasing follicle blood flow.
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    Distribution of TCM syndromes of 183 postoperative patients with cancerous goiter*
    WANG Zhiqiao, L Meng, ZHOU Yu, XIA Zhongyuan
    2015, 38 (9):  645-648.  doi: 10.3969/j.issn.1006-2157.2015.09.014
    Abstract ( 448 )   PDF (1058KB) ( 561 )   Save
    Objective To reveal the rule of TCM syndromes of postoperative patients with cancerous goiter and to provide clinical evidence for TCM treatment on the patients. Methods Based on the literature search and TCM standard of syndromes, Questionnaire of TCM Syndromes of Patients with Cancerous Goiter was established. A cross-sectional study of TCM syndromes was performed on 183 postoperative patients with cancerous goiter. Results Altogether four TCM syndromes existed in 183 patients. The ratio of syndrome of deficiency of both vital qi and yin was 34.43% (n=63), that of syndrome of stagnation of liver qi was 26.23% (n=48), that of syndrome of intermingled phlegm and blood stasis was 24.59% (n=45) and that of syndrome of yang deficiency of spleen and kidney was 14.75%(n=27). Furthermore, distribution of TCM syndrome types showed no statistically differences(P<0.05) among ages and disease courses. Conclusion The rule of distribution of TCM syndromes in postoperative patients with cancerous goiter would guide the syndrome differentiation and treatment.
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