Effects of paeoniflorin on phosphodiesterase subtypes in the hippocampus CA1 zone of rats with syndrome of blood deficiency and liver stagnation*
2016, 39 (4):
Objective To observe the effects of paeoniflorin on the protein expression of phosphodiesterase (PDE) subtypes in the hippocampus CA1 zone of rats with syndrome of blood deficiency and liver stagnation. Methods The rats were divided into blank group, model group, low dose and high dose of paeoniflorin (low-, high-dose) group. Each group had been intragastrically administered since the first day of modeling—low-dose group with 20 mg/kg paeoniflorin, high-dose group with 40 mg/kg, blank group and model group with pure water, for consecutive 21 days. After perfusion fixation, the brains of all rats were dissected and paraffin-emedded sections of brains were studied. The protein expression of PDE1A, PDE2A, PDE4A, PDE5A, PDE9A and PDE10A were detected using immunohistochemistrical methods. Results Compared with blank group, the protein expression of PDE1A, PDE2A, PDE5A and PDE9A of the model group increased significantly(P<0.05,P<0.01,P<0.01,P<0.01),and that of PDE4A, PDE10A declined(P<0.01,P<0.05). Paeoniflorin down-regulated the protein expression of PDE1A, PDE2A, PDE5A and PDE9A remarkably(P<0.05,P<0.01,P<0.01,P<0.01),and up-regulated that of PDE4A, PDE10A(P<0.05). Conclusion The protein expressions of PDE subtypes of rat model with syndrome of blood deficiency and liver stagnation changed remarkably due to the double stimulations of radiation and restraint. The effect of nourishing blood and liver of White Peony Root (Radix Paeoniae Alba, Baishao) suggested be a dynamic balance of multi-target with multi-path, which was proved by the regulations of paeoniflorin on substrates hydrolases resulted in the concentration of the two nucleotides.
Related Articles |