Specific chromatogram of Mongolian medicine compound Sendeng-4 and simultaneous determination of its six index components*
2016, 39 (6):
Objective To establish HPLC specific chromatogram of Sendeng-4, a Mongolian medicine compound, and to simultaneously determine six index components—gallic acid,genipin-1-β-D-gentiobioside, geniposide, (2R, 3R)-dihydromyricetin, rutin, and myricetin. Methods HPLC specific chromatogram was obtained under the following system: Extend-C18 column (250 mm × 4.6 mm, 5 μm), mobile phase of acetonitrile (A) and 0.6 % phosphoric acid aqueous solution (B) , gradient elution, flow rate at 1 mL/min, the column temperature at 25 ℃, detection wavelength at 254 nm, and the injection volume 10 μL. HPLC system for determination was listed as the following: Extend-C18 column (250 mm × 4.6 mm, 5 μm), mobile phase of acetonitrile (A) and 0.6% phosphoric acid aqueous solution (B), gradient elution, flow rate at 0.5 mL/min, the column temperature at 27 ℃, the detection wavelengths at 254 nm and 292 nm, and the injection volume 10 μL. Results More than 90 peaks were detected on HPLC specific chromatogram, from which ten were identified by comparing with standard HPLC chromatogram and six were measured. After determination, the content and the peak area of each component had a good linear relationship, with correlation coefficients 0.999 9, average recovery of 98.29%~103.75% and RSD 1.44%~2.97%. Conclusion The method established is accurate, simple and repeatable, and can be used to control the quality of Mongolian medicine compound Sendeng-4.
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