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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 July 2016, Volume 39 Issue 7 Previous Issue    Next Issue
    ZHANG Zhongjing’s diagnosis on blood stasis pattern compared to diagnostic standard of modren Chinese medicine*
    ZHAO Fangzhou, CHEN Jiaxu
    2016, 39 (7):  536-540.  doi: 10.3969/j.issn.1006-2157.2016.07.002
    Abstract ( 454 )   PDF (1146KB) ( 320 )   Save
    Blood stasis pattern is a commonly-seen pattern in TCM clinical practice. The specific symptoms and diagnosis of blood stasis pattern were recorded in two TCM classics, Treatise on Febrile Diseases (Shang Han Lun) and Synopsis of Golden Chamber (Jin Gui Yao Lüe), both written by Zhang Zhongjing, which provided important foundation for clinical research of blood stasis pattern. To provide a clear train of thought about the characteristics of blood stasis pattern described by Zhang Zhongjing, and to study the development and changes of blood stasis pattern diagnosis from Han Dynasty to modern times, this study systematically generalized statements about blood stasis pattern from those two books according to modern TCM diagnostics, summarizing and analyzing characteristics of blood stasis pattern by Zhang Zhongjing, and compared the diagnosis principles of blood stasis pattern in Treatise on Febrile Diseases(Shang Han Lun) and Synopsis of Golden Chamber (Jin Gui Yao Lüe) with reference to modern principles.
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    Identifying patterns of warm diseases: a proposed new theoretical system*
    AI Jun, DAI Ming, CHEN Sheng, HUANG Biling, HUANG Yiling, WANG Zhiwei, ZHONG Ni
    2016, 39 (7):  541-544.  doi: 10.3969/j.issn.1006-2157.2016.07.003
    Abstract ( 464 )   PDF (1191KB) ( 263 )   Save
    This paper proposes a new theoretical system for pattern identification of warm diseases, which consists of causal pattern identification and mechanism pattern identification. Mechanism pattern identification can be further divided into basic mechanism, systematic and symptomatic pattern identification. Basic mechanism of warm diseases arises from conflict (between healthy and pathogenic qi) and damage (to healthy qi). Heat is divided into flaming heat and stagnating heat in the former pathogenesis while it is divided into warm heat and damp heat in the latter one. Pattern identification Methods such as the Defense, Qi, Nutrient, and Blood pattern identification and the Triple Energizer pattern identification should be integrated with the basic mechanism and symptomatic pattern identification, so that a more comprehensive theoretical system for pattern identification of warm diseases would be achieved, which would also help its clinical application.
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    Literature review on “yin-yang attribute of dryness pathogen”: a bibliometric analysis*
    CHEN Yifan, XU Yichen, KONG Yurong, LI Shixin, XU Xiaoying, GUO Xiazhen
    2016, 39 (7):  547-551.  doi: 10.3969/j.issn.1006-2157.2016.07.005
    Abstract ( 411 )   PDF (1148KB) ( 237 )   Save
    Objective To provide theoretical basis for clinical treatment of diseases caused by dryness pathogen and to offer a new understanding of “yin-yang attribute of dryness pathogen” in traditional Chinese medicine (TCM) by collecting and analyzing ancient and modern literature on yin-yang attribute of dryness pathogen using bibliometric methods.Methods After determining search terms and search criteria, literature before 1949 was retrieved from the electronic Chinese Medical Classics (Fifth Edition), and contemporary literature was collected from the CNKI database and Super Mobile Library to conduct statistics analysis. Results There were 36 entries identified in Chinese Medical Classics (Fifth Edition) and 34 entries from CNKI and Super Mobile Library, in which statements about the “yin-yang attribute of dryness pathogen” were varied. Conclusion According to the analysis, the viewpoint of “dryness pathogen belongs to yin” is better received than the opposite view, “dryness pathogen belongs to yang”, and this study also offers supports for this view. Although the latter view is less popular, it still has clinical value.
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    Regulatory effect of Zhuanggu Zhitong Capsule on Treg/Th17 balance in rats with osteoporosis induced by ovariectomy*
    ZENG Ying, LI Weijuan, ZHANG Wenjuan, LI Jinping, LEI Xiaoming, MO Xinmin
    2016, 39 (7):  555-561.  doi: 10.3969/j.issn.1006-2157.2016.07.007
    Abstract ( 355 )   PDF (2156KB) ( 254 )   Save
    Objective To investigate the anti-postmenopausal osteoporosis mechanism of Zhuanggu Zhitong Capsule (Bone-strengthening Pain-relieving Capsule, ZGZTC) in regulating Treg/Th17 balance in bone tissue.Methods Seventy-two 8-month-old SD female rats were allocated randomly into sham operation group, model group, estradiol group (0.21 mg/kg), ZGZTC high-dose group (1.28 g/kg), ZGZTC medium-dose group (0.64 g/kg) and ZGZTC low-dose group (0.32 g/kg). There were 12 rats in each group. Bilateral ovariectomy was performed on rats in all groups except for the sham operation group. After administration for 18 weeks, all rats were anaesthetized intraperitoneally with hydrazine hydrate. The content of E2, IL-10 and IL-17A was measured with ELISA in left femur. Gene expression of Foxp3, RORγt, IL-17A and IL-10 in right femur was detected by using RT-PCR, and the number of cell with RORγt or Foxp3 was counted with immunohistochemistry in left tibia. Results Zhuanggu Zhitong Capsule significantly increased E2 and IL-10 levels in bone tissue (P<0.01), reduced the level of IL-17A in bone tissue (P<0.05 or P<0.01). It significantly increased the Foxp3 and IL-10 mRNA expression and inhibited the RORγt mRNA expression in bone tissue (P<0.05). The percentage of cell with Foxp3 was significantly increased by ZGZTC in bone tissue (P<0.05), and the percentage of cell with RORγt was significantly reduced (P<0.05 or P<0.01). The ratio of Foxp3 to RORγt presented a positive correlation with the E2 level (r=0.990 5,P<0.01). Conclusion Zhuanggu Zhitong Capsule could downregulate the number and function of Th17, upregulate the number and function of Treg in bone tissue in ovariectomized rats partly via improving the E2 level.
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    Spatial distribution analysis of the tranquilizing effect of Image Thinking based on the alpha band EEG power*
    LI Shenyi, HU Qingchuan, WANG Weiwei, HAN Aiqing, JIA Dexian, ZHANG Haibo, WANG Yanping, ZHANG Weibo, WEI Yulong
    2016, 39 (7):  562-567.  doi: 10.3969/j.issn.1006-2157.2016.07.008
    Abstract ( 324 )   PDF (1977KB) ( 232 )   Save
    Objective To analyze the effects of different kinds of Image Thinking on brain functional area by measuring alpha band electroencephalograph (EEG) power.Methods 48 college students were enrolled and divided into two groups. Subjects in the experiment group received three kinds of Image Thinking intervention, while those in the control group did not receive any intervention. Alpha band absolute power of two groups was measured to compare and analyze. Results The alpha band absolute power value of the experiment group was higher than that in the control group at occipital lobe, parietal lobe, and temporal lobe in both active and resting state (P<0.05). The power in α frequency range was significantly different in three kinds of Image Thinking status. When imagining “I am rambling in the rose bushes”, there was statistically significant difference in contralateral lead in (FP1-FP2), (F7-F8), (C3-C4), (P3-P4), (T5-T6), and (O1-O2) (P<0.05). Moreover, power in the right hemisphere was higher than left hemisphere, and after active stage the power of occipital lobe reduced. When imagining “the spring sunshine is warm on my body”“My every pore is breathing freely”, there was statistically significant difference in contralateral lead in (F7-F8) and (P3-P4) (P<0.05). The alpha band absolute power increased at occipital lobe after active stage, then the power increased to peak at final resting state. Conclusion Image thinking exhibits a significant tranquilizing effect according to the reflection of the high power of alpha band at occipital lobe and parietal lobe. Different kinds of feeling effect caused by Image Thinking maybe related to the function of every brain area.
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    Effects of Herba Ephedra and Fructus Schisandrae combined with dexamethasone on angiogenic factors and inhibitors in rats with idiopathic pulmonary fibrosis*
    ZHANG Xiaojuan, ZHANG Tian, TIAN Weilan, LI Rui, ZHAI Huaqiang
    2016, 39 (7):  568-574.  doi: 10.3969/j.issn.1006-2157.2016.07.009
    Abstract ( 342 )   PDF (2353KB) ( 307 )   Save
    Objective To observe the effects of Mahuang(Herba Ephedra) and Wuweizi(Fructus Schisandrae) combined with dexamethasone(DXM)on expression of angiogenic factors and inhibitors in rats with idiopathic pulmonary fibrosis (IPF).Methods Wistar male rats of SPF level (n=106) were divided randomly into sham operation group, model group, Mahuang group, Wuweizi group, Mahuang+DXM group, Wuweizi+DXM group and DXM group. The model of IPF was established by using instillation of bleomycin (7 mg/ kg) except for the sham operation group. Each group was administered intragastrically of corresponding drug. All rats were sacrificed on the 7th and 28th day via abdominal aorta blood-letting, and the pathological changes of lung tissue were evaluated with HE staining and the expression of hypoxia-inducible factors 1α(HIF-1α), platelet derived growth factor(PDGF), pigment epithelium-derived factor(PEDF) and endostatin in rat serum were also measured with ELISA. Results Compared with the sham operation group, the expression of HIF-1α,PDGF, PEDF and endostatin in model group significantly increased (P<0.01); the HIF-1α content on the 28th day was higher than that on the 7th day, and the PEDF and endostatin content were lower than that on the 7th day. The expression of HIF-1α and PDGF significantly decreased in Mahuang group and Wuweizi group compared with the model group, and the expression of PEDF and endostatin increased(P<0.05). Compared with Mahuang group on the 28th day,the expression of HIF-1α and PDGF on the 7th day increased markedly, but the expression of PEDF and endostatin on the 28th day was lower than those on the 7th day. In the Wuweizi group the changes in the expression of HIF-1α,PDGF, PEDF and endostatin were opposite to Mahuang group. The expression of HIF-1α and PDGF significantly decreased in Mahuang+DXM group and Wuweizi+DXM group compared with the model group(P<0.05), and the expression of PEDF and endostatin increased. In Wuweizi+DXM group the HIF-1α content was significantly higher than those in DXM group while the PEDF content was significantly lower(P<0.05). Conclusion Regulatory factors on angiogenesis lose their balance in rats with IPF. Mahuang and Wuweizi could achieve inhibition of the expression of HIF-1α and PDGF, and selectively promote the expression of PEDF and endostatin. When combined with DXM, the selectivity might be weakened in different pathological stages.
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    Effects of Bushen Yifei Xiaozheng Decoction on the expression of key molecular genes and proteins in Caspase-12 signaling pathway in IPF rats*
    YANG Yingxi, CHAI Limin, WU Tiantian, GAO Weihua, ZHU Ziheng, XU Fang, YU Jiajun, YAN Jun
    2016, 39 (7):  575-579.  doi: 10.3969/j.issn.1006-2157.2016.07.010
    Abstract ( 388 )   PDF (2119KB) ( 205 )   Save
    Objective To investigate the effects of Bushen Yifei Xiaozheng Decoction (kidney-tonifying lung-boosting stasis-resolving decoction) on the expression of key genes and proteins in Casepase-12 signaling pathways in rats with idiopathic pulmonary fibrosis (IPF).Methods IPF was induced with bleomycin in rats except for the normal group. Pirfenidone was used as positive control, while Bushen Yifei Xiaozheng Decoction (TCM) was administered during (TCM preventive group) and after (TCM treatment group) the model established and throughout the whole experiment (TCM preventive and treatment group). Pathological change in the rat lungs was observed with Masson staining, meanwhile the expression of molecular genes and proteins involved in Caspase-12 signaling pathways was detected by using Western blot and Real-time PCR. Results Masson staining revealed that the degree of fibrosis in TCM groups improved significantly and alveolar structure changed little. Protein and gene expression of Caspase-12 pathway in the model group was higher than those of normal group (P<0.05). In addition, the protein and gene expression of pirfenidone group and TCM groups were less than the model group (P<0.05 or P<0.01), especially TCM preventive and treatment group. Conclusion Bushen Yifei Xiaozheng Decoction could inhibit the process of pulmonary fibrosis via acting on the gene expression of Caspase-12 pathway and releasing the endoplasmic reticulum stress.
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    Effect and mechanism of oldenlandia on Cxcl1 gene expression in macrophages*
    YUE Xiaoning, SHI Yang, ZANG Shizhu
    2016, 39 (7):  580-585.  doi: 10.3969/j.issn.1006-2157.2016.07.011
    Abstract ( 361 )   PDF (2661KB) ( 239 )   Save
    Objective To explore the immunoregulatory effect of Oldenlandia (Hedyotis diffusa Villd.), by investigating on the expression of macrophage cytokines and on TLR-4 inflammatory signal transduction pathway, aiming to propose a new experimental and theoretical evidence for the use of oldenlandia.Methods Three common cytokines, such as IL-6, MCP-1 and Cxcl1 in macrophage cells were detected by using RT-PCR after treatment with oldenlandia solution. Autodock software was used to analyze the correlation between the main components and TLR-4 so as to explore the mechanism of expression changes of the cytokines. Results Oldenlandia solution could upregulate the expression of Cxcl1, which might depend on the combination of TLR4 and stigmasterol-beta-D-glucoside, trans-4-methoxycinnamyl alcohol, and asperulosidic acid. Conclusion Oldenlandia could activate TLR-4 signal pathway and thus affect the expression of Cxcl1 and function of macrophage. It can be inferred that oldenlandia could regulate immunity, which could serve as the theoretical rationale for the development of new drugs in the laboratory.
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    Inhibition of Potentilla parvifolia on alpha-glucosidase and aldose reductase*
    SUN Yuxia, ZENG Yang, ZHANG Qianru, YU Tao, LI Meihua,ZHAO Deping
    2016, 39 (7):  586-589.  doi: 10.3969/j.issn.1006-2157.2016.07.012
    Abstract ( 442 )   PDF (1149KB) ( 209 )   Save
    Objectiv To study the inhibitory effects on α-glucosidase (AG) and aldose reductase (AR) extracted from Potentilla parvifolia in different solvents, and to find out natural inhibitors of AG and AR.Methods With a combined vitro and vivo experiment design, this study measured the in vitro inhibitory effects of AG and AR extraction from Potentilla Parvifolia in three solvents: ethyl acetate (total flavonoids), petroleum ether and water through AG inhibitors and AR inhibitory activity screening models. In vivo effects of total flavonoids from Potentilla Parvifolia on impaired glucose tolerance of mice were also measured.Results Extraction of Potentilla Parvifolia in total flavonoids demonstrated a stronger inhibitory effect on AR and AG. Its inhibitory effect on AG was higher than that of acarbose. Meanwhile, both high and low dose of total flavonoids improved glucose tolerance in mice. Conclusion Total flavonoids in Potentilla parvifolia exhibits inhibitory effects on AG and AR
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    Decomposition analysis on extraction rate changes of liquiritin,hyperoside and luteoloside in SimiaoYong’an Decoction*
    HU Shaowei, JIANG Yanyan, GAO Yaochun, WANG Chong, CHEN Luxiao,LIU Bin
    2016, 39 (7):  590-594.  doi: 10.3969/j.issn.1006-2157.2016.07.013
    Abstract ( 370 )   PDF (1522KB) ( 199 )   Save
    Objective To investigate the influence of different decomposed combinations of Simiao Yong’an Decoction(four-ingredient health-restoring decoction) on the extraction rates of liquiritin, hyperoside and luteoloside.Methods The extraction rates of liquiritin, hyperoside and luteoloside were determined with HPLC using a SunfireTMC18 analytical column(4.6mm×150mm,5μm) in decomposed formula of Simiao Yong’an Decoction. The mobile phase was composed of aqueous phosphoric acid (0.1%) and acetonitrile using anisocratic elution.The column temperature was 35℃. The flow rate was 1.0 mL/min while the detection wavelength was set at 276 nm(liquiritin)and 350 nm(hyperoside and luteoloside). The contents and the extraction rates of liquiritin, hyperoside and luteoloside from the original and dismantled formula were detected and analyzed.Results Compared with the single herb, the more ingredients it contained, the higher extraction rates of liquiritin, hyperoside and luteoloside. When the whole ingredients were stewed together, the extraction rates of these 3 components reached the peak.Conclusion The different decomposed SimiaoYong’an Decoction can significantly improve the extraction rates of liquiritin, hyperoside and luteoloside,which indicated the validity and rationality of compound compatibility from the extraction rates of chemical compositions.
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    Efficacy and safety of Shenfu injection treating bradycardia:a systematic review with meta-analysis of randomized controlled trials*
    ZHANG Yili, JIA Min, XIE Yanming, LIAO Xing, WANG Fengjiao
    2016, 39 (7):  595-604.  doi: 10.3969/j.issn.1006-2157.2016.07.014
    Abstract ( 379 )   PDF (2521KB) ( 463 )   Save
    Objective To evaluate the efficacy and safety of Shenfu injection in the treatment of bradycardia.Methods Randomized controlled trials (RCT) on Shenfu injection treating bradycardiawas retrieved from CNKI database, Wanfang Database,VIP Database,CBM,PubMed, EMbase, and Clinical Trials.gov. Cochrane Handbook was used to assess methodological quality, and data were analyzed using RevMan5.3 Software.Results 13 trials involving 978 patients were included into the analysis. The overall methodological quality was low as most studies were evaluated as B level and one study was C level. Meta-analysis showed that Shenfu injection was better than atropine(RR=1.27, 95%CI 1.09~1.48, P=0.002)in terms of the overall effect (relief of symptoms and improvement of heart rate), but there were no statistic difference between using atropine alone andusing Shenfu injection and atropine together(RR=0.84, 95%CI 0.73~0.97, P=0.30). Compared with Shenfu injection in polarized solution, Shenfu injection can improve the clinical symptoms better(RR=0.52, 95%CI 0.40~0.67, P=0.01), but there was no difference in the heart rate increase (RR=0.57,95%CI 0.32~1.20,P=0.06). Safety:Adverse reactions were reported in four studies, with dry mouth being the most common one. Conclusion In view of the quality of the current research, the efficacy of treating bradycardia with Shenfu injection cannot be ensured. In the future, high quality studies are needed to confirm the efficacy of Shenfu injection.
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    Stimulating effects of embedded material on acupoints in healthy human using MRI T2-mapping scanning over time and location*
    NIE Hongfang, LIANG Xin, HOU Xunrui
    2016, 39 (7):  605-609.  doi: :10.3969/j.issn.1006-2157.2016.07.015
    Abstract ( 235 )   PDF (2031KB) ( 226 )   Save
    Objective To observe changes in the stimulating effects of micro-invasion catgut embedding on different acupoints in healthy human over time and location, providing reference data for interval period of micro-invasion catgut embedding,and To study the feasibility of MRI T2-mapping technology used to measure stimulating effect of embedding material.Methods Before and after the left acupoints sanyinjiao (SP 6) and zusanli (ST 36) were treated with micro-invasion catgut embedding on 8 healthy subjects, 8 echo sequence images were collected by using MRI scan. Sources of 8-echo sequence images were sent to the work station to get T2-mappings, and test the T2 value on T2-mappings, analyzing the characteristics of T2 value with time variation while comparing the difference of catgut absorption in SP 6 and ST 36. Results In both two acupoints, T2 values on the 8th hour, the 3th, the 7th and the 14th day after being catgut-embedded were all higher than those at the time before catgut-embedding (P<0.01), T2 valuse on the 21th, the 28th and the 35th day after being catgut-embedded presented with no significant difference compared with the time before catgut-embedding (P>0.05). After being catgut-embedded, the peaks of T2 value appeared on the 3th day. Peak of T2 value in ST 36 was higher than that in SP 6, and T2 value in ST 36 decreased in a curve steeper than that in SP 6. It both took 21 days for T2 value in two acupoints to decrease to the level before catgut-embedding. Conclusion Catguts absorption period in ST 36 and SP 6 are the same. If applying micro-invasion catgut-embedding in acupoints on normal body limbs at the same condition, the catgut-embedding interval period is probably about 21 days. MRI T2-mapping technology could be applied to observe stimulating effect of embedding material after acupoint embedding.
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    Relationship between TCM patterns and high-resolution CT imaging features of primary Sjogren’s syndrome patients with interstitial lung disease*
    LIU Yang, DONG Zhenhua, HAO Weixin
    2016, 39 (7):  610-613.  doi: 10.3969/j.issn.1006-2157.2016.07.016
    Abstract ( 390 )   PDF (1143KB) ( 387 )   Save
    Objective To analyze the relationship between the clinical manifestations of TCM patterns and high-resolution CT (HRCT) imaging features in primary Sjogren’s syndrome (pSS) patients with interstitial lung disease(pSS-ILD).Methods A retrospective study on 75 cases with pSS-ILD was conducted to analyze the distribution of TCM patterns and chest HRCT staging and their correlation. Results Among 75 cases, there were three principal TCM patterns, which were deficiency pattern, excess pattern and deficiency-excess complex pattern.They could be further divided into 8 patterns in which and lung qi and yin deficiency pattern were the most frequent(29.3%), lung qi and yin deficiency with phlegm obstructing lung pattern(28%). Correlation analysis showed that the more severe the lesion degree of HRCT imaging was, the more frequent the lung qi and yin deficiency pattern was(85.7%, P=0.020).Conclusion TCM pathogenesis of pSS-ILD is a deficiency-excess complex pattern with a deficiency root and excess manifestation. There is certain relevance between TCM pattern and the lesion degree in HRCT imaging.
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